Mulberry leaf extract reduces damage by regulating the oxidative response, immune response and intestinal flora of largemouth bass (Micropterus salmoides) under chronic heat stress
Frontiers in Marine Science,
Journal Year:
2025,
Volume and Issue:
12
Published: Feb. 7, 2025
Chronic
heat
stress
(HS)
induces
oxidative
damage,
low
immunity,
and
intestinal
flora
disturbance
of
fish,
posing
great
challenges
to
the
aquaculture
industry.
As
an
important
plant
extract,
mulberry
leaf
extract
(MLE)
has
been
shown
have
antioxidant
immune-boosting
properties.
This
study
evaluated
protective
effect
dietary
MLE
on
HS-induced
liver
injury
in
Largemouth
bass
(
Micropterus
salmoides
)
based
damage
parameters,
immune
composition.
Fish
were
randomly
assigned
into
three
groups:
CON
group
(ambient
temperature,
26°C,
basal
diet),
HS
(33°C,
+
diet
supplemented
with
9
g/kg
MLE).
significantly
decreased
final
body
weight,
specific
growth
rate,
villus
length,
muscular
layer
thickness,
ACE
Chao1
indices,
lipase,
trypsin,
total
superoxide
dismutase
(T-SOD),
glutathione
peroxidase
(GPx),
alkaline
phosphatase
(AKP),
lysozyme
(LZM)
activities,
but
increased
feed
conversion
aspartate
aminotransferase
(AST)
activity,
reactive
oxygen
species
(ROS),
malondialdehyde
(MDA),
complement
3
(C3)
contents,
relative
abundances
Proteobacteria
Plesiomonas
.
Compared
group,
improved
GPx,
ACP,
LZM
indices
intestine,
numbers
vacuoles
inflammatory
cells,
AST
GLU,
MDA,
ROS
contents.
Dietary
also
up-regulated
mRNA
expressions
gpx
,
tg
f-β
il-10
jnk2
bcl-2
down-regulated
il-8
tnf-α
caspase-3
atf4
chop
ire1,
traf2
jnk1
TRPV
MCU
VDAC
liver.
In
conclusion,
supplementation
could
improve
growth,
diversity
heat-stressed
largemouth
bass,
alleviate
hepatic
injuries
by
regulating
inflammation,
stress,
apoptosis,
providing
a
theoretical
basis
for
development
as
treatment
against
HS.
Language: Английский
Effects of Mango Seed (Mangifera indica) Powder on Growth Performance, Immune Response, Gut Morphology, and Gene Expression of Nile Tilapia (Oreochromis niloticus)
Fishes,
Journal Year:
2024,
Volume and Issue:
9(12), P. 514 - 514
Published: Dec. 16, 2024
This
study
explored
the
effects
of
mango
seed
(MS)
powder
supplementation
on
growth,
immune
response,
gene
expression,
and
intestinal
morphology
Nile
tilapia
(Oreochromis
niloticus)
over
an
8-week
period.
A
total
300
fingerlings
(average
weight
15.29
±
0.05
g)
were
divided
into
five
treatment
groups
fed
either
a
basal
diet
or
one
four
experimental
diets
containing
MS
at
concentrations
10
(MS10),
20
(MS20),
40
(MS40),
80
(MS80)
g
kg−1.
The
results
demonstrated
that
MS-supplemented
experienced
significant
improvements
(p
<
0.05)
in
gain
(WG),
specific
growth
rate
(SGR),
survival
(SR)
compared
to
control
group
(0
kg−1
MS).
MS-treated
also
showed
increase
height
branching
villi
along
entire
length
intestine,
as
well
significantly
higher
villus-to-crypt
depth
ratio
(V/C),
indicating
enhanced
health
functionality.
Moreover,
although
did
not
peroxidase
activity,
it
lead
activity
skin
mucus
serum
lysozyme,
with
upregulated
expression
immune-related
(IL-1,
IL-8,
LBP)
antioxidant
genes
(GST-α,
GPX,
GSR).
Polynomial
regression
analysis
identified
optimal
dosage
36.43–45
for
effectively
improving
immunity,
immuno-oxidant
tilapia.
These
emphasize
promising
natural
supplement
and,
potentially,
other
freshwater
fish
widely
used
aquaculture.
Language: Английский