Tilapia aquaculture, emerging diseases, and the roles of the skin microbiomes in health and disease

Aquaculture International, Journal Year: 2023, Volume and Issue: 31(5), P. 2945 - 2976

Published: April 25, 2023

Abstract Aquaculture is playing an increasingly important role in global food security, especially for low-income and food-deficit countries. The majority of aquaculture production occurs freshwater earthen ponds tilapia has quickly become one the most widely adopted culture species these systems. Tilapia are now farmed over 140 countries facilitated by their ease production, adaptability to a wide range environmental conditions, fast growth, high nutritional value. Typically, have been considered hardy, disease resilient species; however, increasing with subsequent threats industry as intensified. In this review, we discuss focus on Bangladesh top producing countries, highlight problems associated treatment approaches them, including misuse antimicrobials. We address key missing component understanding health processes sustainable aquaculture, specifically played microbiome. Here examine importance microbiome supporting health, focused symbiotic microbial community fish skin mucosal surface, abiotic biotic factors that influence microbiome, shifts diseased states. also identify conserved taxa microbiomes may be used indicators status offering new opportunities mitigate manage optimize growing conditions farming practices.

Language: Английский

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Microbiomes in the context of developing sustainable intensified aquaculture

Frontiers in Microbiology, Journal Year: 2023, Volume and Issue: 14

Published: June 23, 2023

With an ever-growing human population, the need for sustainable production of nutritional food sources has never been greater. Aquaculture is a key industry engaged in active development to increase line with this while remaining terms environmental impact and promoting good welfare health farmed species. Microbiomes fundamentally underpin animal health, being part their digestive, metabolic defense systems, latter case protecting against opportunistic pathogens environment. The potential manipulate microbiome advantage enhancing intriguing prospect that gained considerable traction recent years. In review we first set out what known about role aquaculture systems across phylogenetic spectrum cultured animals, from invertebrates finfish. view reducing footprint tightening biological physical control, investment “closed” on rise, but little how microbial these closed affect organisms. Through comparisons microbiomes dynamics phylogenetically distinct animals different focus communities functionality order identify features within be harnessed optimizing healthy intensified support future aquaculture.

Language: Английский

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Metagenomic assessment of the diversity and ubiquity of antimicrobial resistance genes in Bangladeshi aquaculture ponds

Aquaculture Reports, Journal Year: 2023, Volume and Issue: 29, P. 101462 - 101462

Published: Jan. 24, 2023

In Bangladesh, fish provide over 60% of animal-source food with 56.2% this coming from aquaculture produced predominantly in rural freshwater ponds. Increasing demand for products is driving intensification and resulting higher disease prevalence, posing a risk to security. Biosecurity often absent practices Bangladesh antibiotics are commonly used treat prevent outbreaks. Antibiotics administered incorrectly - key factor associated the development antimicrobial resistance (AMR). AMR can be disseminated rapidly within microbial ecosystems via mobile genetic elements, humans animals infected pathogens as treatments become ineffective. Early detection understanding spread genes (ARGs) critical both security, human health protection safety. Here, we apply metagenomic approach assess ARG composition pond water six finfish (tilapia pangasius) farms Mymensingh division North-central Bangladesh. We found communities ponds had similar alpha beta diversities, multiple ARGs predicted confer eighteen different classes antimicrobials. The most common conferred aminoglycosides sulphonamides were present taxa pathogens. This diversity potentially confers wide variety antibiotic questions effectiveness current future treatment diseases earthen compositions between each farm similar, which may relate parallels farming creating selection pressures thus comparable populations. Without more controlled towards usage, will inevitably further exacerbate challenges treating preventing outbreaks production intensifies

Language: Английский

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A Multiplexed, Tiled PCR Method for Rapid Whole-Genome Sequencing of Infectious Spleen and Kidney Necrosis Virus (ISKNV) in Tilapia

Viruses, Journal Year: 2023, Volume and Issue: 15(4), P. 965 - 965

Published: April 14, 2023

Tilapia farming is one of the most important sectors in aquaculture worldwide and major importance to global food security. Infectious spleen kidney necrosis virus (ISKNV) has been identified as an agent high morbidity mortality, threatening tilapia aquaculture. ISKNV was detected Lake Volta, Ghana, September 2018 spread rapidly, with mortality rates between 60 90% losses more than 10 tonnes fish per day. Understanding evolution viral pathogens for control strategies. Here, we developed a tiled-PCR sequencing approach whole-genome ISKNV, using long read enable field-based, real-time genomic surveillance. This work represents first use whole genome recovery viruses aquaculture, longest target (>110 kb dsDNA) date. Our protocol applied field samples collected from outbreaks four intensive cage culture systems across October May 2022. Despite low mutation rate dsDNA viruses, 20 single nucleotide polymorphisms accumulated during sampling period. Droplet digital PCR minimum requirement template sample recover 50% at 275 femtograms (2410 templates 5 µL reaction). Overall, provides informative tool assist disease

Language: Английский

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Influence of host phylogeny and water physicochemistry on microbial assemblages of the fish skin microbiome

FEMS Microbiology Ecology, Journal Year: 2024, Volume and Issue: 100(3)

Published: Feb. 14, 2024

Abstract The skin of fish contains a diverse microbiota that has symbiotic functions with the host, facilitating pathogen exclusion, immune system priming, and nutrient degradation. composition microbiomes varies across species in response to variety stressors, however, there been no systematic analysis these studies evaluate how factors shape microbiomes. Here, we examined 1922 from 36 included 98 nine rearing conditions investigate associations between microbiome, species, water physiochemical factors. Proteobacteria, particularly class Gammaproteobacteria, were present all marine freshwater Acinetobacter, Aeromonas, Ralstonia, Sphingomonas Flavobacterium most abundant genera within microbiomes, Alteromonas, Photobacterium, Pseudoalteromonas, Psychrobacter Vibrio saltwater fish. Our results show different culturing (rearing) environments have small but significant effect on bacterial community compositions. Water temperature, pH, dissolved oxygen concentration, salinity significantly correlated differences beta-diversity not necessarily alpha-diversity. To improve study comparability provide recommendations for approaches analyses sequencing data reproducibility.

Language: Английский

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Composition and function of the skin microbiota were altered of red leg syndrome in cultured bullfrog (Rana catesbeiana)

Aquaculture Reports, Journal Year: 2023, Volume and Issue: 29, P. 101487 - 101487

Published: Feb. 7, 2023

Skin microbiota play an important role in skin barrier function and are associated with host health. The study of the is beneficial for managing disease microbiota. Bullfrog (Rana catesbeiana) cultured animal. Bacterial diseases like red leg syndrome (RLS) often occur bullfrog cause huge economic losses around world. However, appearance RLS unknown. Here we compare healthy by 16 S rRNA microbiome profiles. bacterial community diversity was significantly different between group. Proteobacteria, Bacteroidota, Firmicutes were most abundant phyla skin. Compared bullfrogs, bullfrogs had high abundance Acinetobacter, Chryseobacterium, Elizabethkingia, Flavobacterium Streptococcus microbiota, but relative Cetobacterium, Bacteroides Shewanella overrepresented bullfrog. Tax4Fun prediction showed that genes Nucleotide metabolism, Translation, Glycan biosynthesis metabolism exhibited higher bullfrogs. results suggested changed composition predictive This first to report on relationship bullfrog, which can expand our understanding communities farming from perspective microbial ecology help prevent manage farming.

Language: Английский

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Nutritional Fish Diseases in Aquaculture: A Human Health Hazard or Mythical Theory: An Overview

European Journal of Nutrition & Food Safety, Journal Year: 2023, Volume and Issue: 15(8), P. 41 - 58

Published: July 21, 2023

To consume or not to farmed fish diagnosed with nutritional disorders within an aquaculture culture system is a question that resonates fisheries and experts worldwide. Proper health management in should be critical area of concern for all farmers. It has become fundamental requirement achieving sustainable production. However, many farmers, particularly developing countries lack knowledge about diseases how effectively manage them on the farm. Like human health, fish’s systems daily priority Nutritional are often overlooked by majority even at commercial level. Most Fish farmers may realize disease prevention can achieved through use well-formulated feeds meet requirements growth. Additionally, maintaining good water quality implementing best practices essential ensure stock remains healthy system. Since different species have varying dietary requirements, it important formulate right diet based specific being cultured. contain necessary nutrients opportunistic resistance. Furthermore, familiar early diagnosis techniques disorders. This ensures harvested reaching market does raise concerns safety products. Failure diagnose associated poor diets create doubts rapidly growing industry. Therefore, this review aims explore species, impact deficiencies public causes disorders, viable therapies managing these fish. The data article were collected from various secondary sources, including internationally reputable peer-reviewed articles, conference proceedings reports, internet searches, more. findings highlight importance providing information nutrition-related control, as been found detrimental health. Moreover, affected entered global market, raising questions their crucial prioritize maintain consumer confidence globally. study recommends further research into potentially lethal effects diseases, when consumed large quantities environment especially dead discarded backwaters aquatic ecosystem.

Language: Английский

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Nutritional Requirements for Healthy Aquatic Animal

Published: Jan. 1, 2025

Language: Английский

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Seasonal dynamics and factors shaping microbiomes in freshwater finfish earthen aquaculture ponds in Bangladesh

Environmental Microbiome, Journal Year: 2025, Volume and Issue: 20(1)

Published: March 31, 2025

Abstract Background The pondwater microbiome is believed to play a key role in fish health, including shaping mucosal surface microbiomes that help protect against disease. How different physiochemical features relating season, geographical locations, as well crop species shape the pond water finfish aquaculture system, not established. Pangasius ( Pangasianodon hypophthalmus ) and tilapia Oreochromis niloticus are two of most widely farmed disease major impediment expansion their production. We applied 16S 18S rRNA metabarcoding assess how physicochemistry location pangasius earthen ponds Bangladesh. Results Planctomycetota, Pseudomonadota Actinomycetota were dominant bacterial phyla while Stramenopiles Alveolata microeukaryotes (divisions) water. relative abundance Planctomycetota was higher compared with ponds, Actinomycetota, relatively ponds. Tilapia also exhibited microbial diversity at its lowest winter (and/or monsoon) highest pre-monsoon period. community structures differed across seasons, culture systems, species, season locations showing strongest effects. Of assessed, temperature pH found have weak but significant effect on content for both shared over 46% ASVs, around 30% ASVs study locations. Conclusion Our findings demonstrate communities systems Bangladesh shaped by location, effects from physicochemistry. results provide insights into dynamic nature environmental influences may be use management improving productivity enhancement overall health. Graphical

Language: Английский

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Bacterial Community Composition and Prevalence of Aeromonas dhakensis in Four Tilapia Freshwater Aquaculture Systems in Malaysia

Fishes, Journal Year: 2025, Volume and Issue: 10(5), P. 204 - 204

Published: May 1, 2025

The tilapia (Oreochromis) aquaculture industry in Malaysia has expanded rapidly to meet the increasing demand for animal protein. However, this growth is challenged by microbial infections, particularly those caused emerging pathogen Aeromonas dhakensis. This study aimed investigate community composition across four distinct farming systems and assess associated water physicochemical parameters, with a focus on detecting presence of A. dhakensis elucidating its environmental associations. Water parameters were measured evaluate conditions, communities characterized through 16S rDNA metabarcoding, was detected using both microbiological molecular approaches. Principal component analysis (PCA) canonical correspondence (CCA) employed explore influence variables dynamics occurrence. Our results indicated that floating cages exhibited higher levels temperature, ammonium, fecal coliform, while cement tanks showed signs nutrient accumulation. PCA revealed degraded quality. A total 45 strains fingerprints isolated. metabarcoding Proteobacteria, Actinobacteria, Planctomycetota as dominant phyla. Alpha diversity did not differ significantly among pond systems, beta variations assemblages systems. CCA identified dissolved oxygen, macronutrients (phosphate, nitrate, nitrite), turbidity significant drivers shaping structure In conclusion, highlights importance factors, levels, potentially influencing pathogenic bacteria such These findings underscore need improved management mitigate disease risks support fish health.

Language: Английский

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