Exploring the interactions between the gut microbiome and the shifting surrounding aquatic environment in fisheries and aquaculture: A review

Environmental Research, Journal Year: 2022, Volume and Issue: 214, P. 114202 - 114202

Published: Aug. 27, 2022

Language: Английский

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Composition and distribution of bacterial communities and antibiotic resistance genes in fish of four mariculture systems

Environmental Pollution, Journal Year: 2022, Volume and Issue: 311, P. 119934 - 119934

Published: Aug. 13, 2022

Fish-related antibiotic resistance genes (ARGs) have attracted attention for their potentially harmful effects on food safety and human health through the chain transfer. However, potential factors affecting these ARGs not been fully explored. In this study, bacterial communities in fish gut, mucosal skin, gill filaments were comprehensively evaluated four different mariculture systems formed by hybrid grouper (Epinephelus fuscoguttatus♀ × E. lanceolatus♂), Gracilaria bailinae, Litopenaeus vannamei using combinations. The results showed that 9 detected gut skin 6 filaments. detection rate of aphA1 was highest, abundance 1.91 10-3 - 6.30 10-2 copies per 16 S rRNA gene. Transposase gene (tnpA-04) all samples with 3.57 3.59 gene, strongly correlated multiple (e.g., aphA1, tet(34), mphA-02). Proteobacteria, Deinococcus-Thermus, Firmicutes, Bacteroidetes dominant phyla systems, accounting 65.1%-96.2% total community. Notably, high relative Stenotrophomonas, a pathogen, elevated 20.5% monoculture system. addition, variation partitioning analysis (VPA) difference between main driving factor distribution differences groupers. This study provides new comprehensive understanding characterization fish-related contamination facilitates assessment risks pathogen taxa to health.

Language: Английский

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Integrated Transcriptome and 16S rDNA Analyses Reveal That Transport Stress Induces Oxidative Stress and Immune and Metabolic Disorders in the Intestine of Hybrid Yellow Catfish (Tachysurus fulvidraco♀ × Pseudobagrus vachellii♂)

Antioxidants, Journal Year: 2022, Volume and Issue: 11(9), P. 1737 - 1737

Published: Aug. 31, 2022

Live fish are often transported in aquaculture. To explore the effects of transport stress, hybrid yellow catfish (Tachysurus fulvidraco♀ × Pseudobagrus vachellii♂) were subjected to simulated treatments (0-16 h) with 96 h recovery after 16-h treatment, and intestinal biochemical parameters, transcriptome, gut microbiota analyzed. Transportation affected number mucus cells led oxidative stress intestine, which activated immune responses. Changes lipid metabolism reflected metabolic adaptation stress. Toll-like receptor signaling, peroxisome proliferator-activated steroid biosynthesis pathways involved response. Gene expression analyses indicated that transport-induced local damage was reversible, whereas disordered recovered more slowly. A 16S rDNA analysis revealed decreased alpha diversity disrupted its homeostasis. The dominant phyla (Fusobacteria, Bacteroidetes) genera (Cetobacterium, Barnesiellaceae) antioxidant, immune, responses host transportation Correlation suggested microbes participate response host-microbiota interaction may trigger multiple events pathways. Our results will be useful for optimizing processes.

Language: Английский

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Exploring the indicator gut microbiota taxa in grass carp (Ctenopharyngodon idella): Correlations with growth rates

Aquaculture, Journal Year: 2024, Volume and Issue: unknown, P. 742080 - 742080

Published: Dec. 1, 2024

Language: Английский

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Feed Regime Slightly Modifies the Bacterial but Not the Fungal Communities in the Intestinal Mucosal Microbiota of Cobia Fish (Rachycentron canadum)

Microorganisms, Journal Year: 2023, Volume and Issue: 11(9), P. 2315 - 2315

Published: Sept. 14, 2023

The bacterial community of the intestinal microbiota influences many host functions, and similar effects have been recently reported for fungal (mycobiota). Cobia is a tropical fish that has studied its potential in marine aquaculture. However, study underreported mycobiota not investigated. We analyzed gut profile present mucosa reared adult cobias fed two diets (frozen pieces (FFPs) formulated feed (FF)) 4 months by sequencing 16S rRNA (V3-V4) internal transcribed spacer-2 (ITS2) regions using Illumina NovaSeq 6000. No significant differences alpha diversity were observed, which was dominated phyla Proteobacteria (~96%) Firmicutes (~1%). FF showed higher abundance 10 genera, mainly UCG-002 (Family Oscillospiraceae) Faecalibacterium, compared to cobia FFPs, 7 Methylobacterium-Methylorubrum Cutibacterium. inferred functions related metabolism, environmental information processing cellular processes; no found between diets. In mycobiota, observed composition Ascomycota (~88%) Basidiomycota (~11%). This first describe communities under captive conditions on different identify genus Ascobulus as new member core mycobiota.

Language: Английский

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Mechanistic insights into the early life stage microbiota of silver pompano (Trachinotus blochii)

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: April 17, 2024

Deep investigations of host-associated microbiota can illuminate microbe-based solutions to improve production in an unprecedented manner. The poor larval survival represents the critical bottleneck sustainable marine aquaculture practices. However, little is known about profiles and their governing eco-evolutionary processes early life stages teleost, impeding development suitable beneficial microbial management strategies. study provides first-hand mechanistic insights into its a tropical teleost model,

Language: Английский

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Gut microbes of a high-value marine fish, Snubnose Pompano (Trachinotus blochii) are resilient to therapeutic dosing of oxytetracycline

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Nov. 14, 2024

Trachinotus blochii is a high-value tropical mariculture species. The present study evaluated the gut microbial impact of therapeutic exposure (80 mg/day/kg biomass for 10 days) to oxytetracycline, most common aquaculture antibiotic in T. blochii. cultivable counts, α-diversity measures taxonomic and functional metagenomics, dysbiosis (MD) index, taxon abundances showed resilience microbiota at 16–26 days treatment. A significant reduction bacterial abundance, diversity measures, Firmicutes Actinobacteria an increase γ-Proteobacteria was recorded on 6th 11th day increased metagenomic stress signatures, decreased beneficial abundances, abundance pathways energy metabolism, MD index indicated short-term transient during initial withdrawal, warranting health management measures. Therapeutic reduced fish pathogens, including Vibrio spp., kanamycin ampicillin-resistant bacteria. Strikingly, oxytetracycline treatment did not tetracycline-resistant counts predicted tetracycline resistance encoding genes gut, illustrating that application would pose risk context antimicrobial short term. Altogether, provides foundation develop suitable minimization tactics sustainable aquaculture.

Language: Английский

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The Anti-Inflammatory Role of Mir-455 in the Immune System by Targeting Egr2 in the Intestine of Hybrid Yellow Catfish (Tachysurus Fulvidraco♀ × Pseudobagrus Vachellii♂) Under Transport Stress

Published: Jan. 1, 2024

Language: Английский

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Deciphering the microbial landscapes in the early life stages of a high-value marine fish, cobia (Rachycentron canadum, Rachycentridae) through high-resolution profiling by PacBio SMRT sequencing

Aquaculture, Journal Year: 2023, Volume and Issue: 582, P. 740503 - 740503

Published: Dec. 25, 2023

Language: Английский

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