Difference in muscle metabolism caused by metabolism disorder of rainbow trout liver exposed to ammonia stress

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 924, P. 171576 - 171576

Published: March 8, 2024

Language: Английский

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Effects of chronic saline-alkaline stress on gill, liver and intestinal histology, biochemical, and immune indexes in Amur minnow (Phoxinus lagowskii)

Aquaculture, Journal Year: 2023, Volume and Issue: 579, P. 740153 - 740153

Published: Sept. 26, 2023

Language: Английский

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Effects of Saline-Alkaline Stress on Metabolome, Biochemical Parameters, and Histopathology in the Kidney of Crucian Carp (Carassius auratus)

Metabolites, Journal Year: 2023, Volume and Issue: 13(2), P. 159 - 159

Published: Jan. 20, 2023

The salinization of the water environment caused by human activities and global warming has increased which brought great survival challenges to aquatic animals. Crucian carp (Carassius auratus) is an essential freshwater economic fish with superior adaptability saline-alkali water. However, physiological regulation mechanism crucian adapting stress remains still unclear. In this study, were exposed or 20, 40, 60 mmol/L NaHCO3 environments for 30 days, effects on kidney evaluated histopathology, biochemical assays metabolomics analysis from renal function, antioxidant capacity metabolites level. Our results showed different degrees damage at exposure concentrations, characterized glomerular atrophy swelling, tubular degranulation, obstruction degeneration, interstitial edema, cell proliferation necrosis. Saline-alkali could change levels several parameters function capacity, including creatinine (CREA), urea nitrogen (BUN), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px) malondialdehyde (MDA). addition, that differential (DMs) involved in various metabolic pathways, phenylalanine, tyrosine, tryptophan biosynthesis, aminoacyl-tRNA purine metabolism, glycerophospholipid sphingolipid glycolysis/gluconeogenesis TCA cycle. general, our study revealed saline-alkaline cause significant changes profiles, induce severe through destroying anti-oxidant system energy homeostasis, inhibiting protein amino acid catabolism, as well disordering metabolism lipid metabolism. This contribute a deeper understanding adverse regulatory adaptation

Language: Английский

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Effect of different concentrations of carbonate on growth performance, intestinal health and hepatic lipid metabolism of Crucian carp

Aquaculture, Journal Year: 2024, Volume and Issue: 589, P. 740990 - 740990

Published: April 24, 2024

Language: Английский

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Transcriptomic Modulation Reveals the Specific Cellular Response in Chinese Sea Bass (Lateolabrax maculatus) Gills under Salinity Change and Alkalinity Stress

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(6), P. 5877 - 5877

Published: March 20, 2023

Salinity and alkalinity are among the important factors affecting distribution, survival, growth physiology of aquatic animals. Chinese sea bass (Lateolabrax maculatus) is an aquaculture fish species in China that can widely adapt to diverse salinities from freshwater (FW) seawater (SW) but moderately highly alkaline water (AW). In this study, juvenile L. maculatus were exposed salinity change (SW FW) stress (FW AW). Coordinated transcriptomic responses gills investigated based on weighted gene co-expression network analysis (WGCNA), 8 11 stress-responsive modules (SRMs) identified for stress, respectively, which revealed a cascade cellular oxidative osmotic gills. Specifically, four upregulated SRMs enriched with induced differentially expressed genes (DEGs) mainly corresponding functions "extracellular matrix" "anatomical structure", indicating strong response water. Both "antioxidative activity" "immune response" downregulated SRMs, comprised inhibited specific DEGs, revealing severely disrupted immune antioxidative under stress. These alkaline-specific not groups only osmoregulation Therefore, results correlated regulation process saline-alkaline water, may have arisen through functional divergence adaptive recruitment will provide vital insights development cultivation

Language: Английский

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Metabolomic responses based on transcriptome of the hepatopancreas in Exopalaemon carinicauda under carbonate alkalinity stress

Ecotoxicology and Environmental Safety, Journal Year: 2023, Volume and Issue: 268, P. 115723 - 115723

Published: Nov. 22, 2023

High carbonate alkalinity is one of the major stress factors for survival aquatic animals in saline-alkaline water. Exopalaemon carinicauda a good model studying adaption mechanism crustacean because its great adaptive capacity to stress. In this study, non-targeted liquid chromatography-mass spectrometry (LC-MS) metabolomics analyses based on high-throughput RNA sequencing (RNA-Seq) were used study metabolomic responses hepatopancreas E. at 12 h and 36 after acute The results revealed that most significantly differential metabolites related lipid metabolism. particular, sphingolipid metabolism was observed h, glycerophospholipid detected linoleic acid metabolic pathway enriched both h. combined transcriptome metabolome analysis showed energy consumption increased resulting significant enrichment AMPK signaling pathways, which contributed maintain homeostasis. Subsequently, provided sufficient supply through cAMP glycerophosphate normal function These findings might help understand molecular mechanisms under stress, thereby promote research development resistant shrimp.

Language: Английский

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Genetic Basis and Identification of Candidate Genes for Alkalinity Tolerance Trait in Spotted Sea Bass (Lateolabrax maculatus) by Genome-Wide Association Study (GWAS)

Marine Biotechnology, Journal Year: 2025, Volume and Issue: 27(1)

Published: Jan. 9, 2025

Language: Английский

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Importance of the ECM–receptor interaction for adaptive response to hypoxia based on integrated transcription and translation analysis

Molecular Ecology, Journal Year: 2024, Volume and Issue: unknown

Published: April 16, 2024

Abstract Low dissolved oxygen (LO) conditions represent a major environmental challenge to marine life, especially benthic animals. For these organisms, drastic declines in availability (hypoxic events) can trigger mass mortality events and thus, act as agents of selection influencing the evolution adaptations. In sea cucumbers, one most successful groups invertebrates, exposure hypoxic triggers adaptive adjustments metabolic rates behaviour. It is unclear, however, how responses are regulated genetic mechanisms underpinning them. Here, we addressed this knowledge gap by assessing regulation (transcription translation) hypoxia cucumber Apostichopus japonicus . Transcriptional translational gene expression profiles under short‐ long‐term low tightly associated with extracellular matrix (ECM)–receptor interaction which laminin collagen likely have important functions. Finding revealed that genes high efficiency (TE) had relatively short upstream open reading frame (uORF) uORF normalized minimal free energy, suggesting cucumbers may respond stress via altered TE. These results provide valuable insights into regulatory confer capacity holothurians survive deficiency also be used inform development strategies for mitigating harmful effects on other invertebrates facing similar challenges.

Language: Английский

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Acute alkalinity stress induces functional damage and alters immune metabolic pathways in the gill tissue of spotted scat (Scatophagus argus)

Aquaculture, Journal Year: 2025, Volume and Issue: unknown, P. 742186 - 742186

Published: Jan. 1, 2025

Language: Английский

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Identification of potential geosmin-binding proteins in grass carp gill based on affinity responsive target stability and tandem mass tag proteomics

Ecotoxicology and Environmental Safety, Journal Year: 2025, Volume and Issue: 291, P. 117832 - 117832

Published: Feb. 1, 2025

The escalating issue of water pollution, especially the accumulation organic off-flavor pollutants, poses significant challenges. Geosmin, a typical compound in aquatic environments, not only compromises quality products but also deters consumers. Its impact extends to organisms, with current research focusing on dose-response and ecotoxicity, while neglecting molecular-level study geosmin-binding proteins. This employs an integrated approach combing affinity-responsive target stability vitro, tandem mass tag proteomics vivo, molecular docking identify proteins gill tissue grass carp (Ctenopharyngodon idella). ARTS analysis identified 56 proteins, predominantly membrane-associated such as catenin beta-1, annexin, integrin beta. Proteomic revealed 256 differentially expressed geosmin-exposure group, 18 common screened by vivo vitro methods. Among these, cathepsin D, interleukin-1 receptors were highlighted potential geosmin targets, annexin demonstrating highest binding affinity silico. provides robust protocol integrating silico approaches elucidate geosmin's tissue, advancing our understanding pollutant-biological interactions enhancing environmental risk assessment accuracy.

Language: Английский

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