Comparative transcriptome analysis identified genes involved in testicular development in Takifugu rubripes

Fish Physiology and Biochemistry, Journal Year: 2025, Volume and Issue: 51(1)

Published: Jan. 6, 2025

Language: Английский

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Transcriptome analyses suggest distinct master sex determination genes in closely related blue catfish and channel catfish

Aquaculture, Journal Year: 2025, Volume and Issue: 603, P. 742364 - 742364

Published: March 6, 2025

Language: Английский

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Synchronously sexual maturity in hermaphrodite fish as revealed by transcriptome analysis in Plectropomus leopardus

Gene, Journal Year: 2024, Volume and Issue: 901, P. 148166 - 148166

Published: Jan. 17, 2024

Language: Английский

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RNA interference with foxl2 leads to upregulation of cyp19b and delayed development of female carp Cyprinus carpio var. koi gonads

Journal of Oceanology and Limnology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 2, 2025

Language: Английский

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Comparative Transcriptomic Analysis of Male and Female Gonads in the Zig-Zag Eel (Mastacembelus armatus)

Fishes, Journal Year: 2025, Volume and Issue: 10(3), P. 117 - 117

Published: March 6, 2025

The zig-zag eel (Mastacembelus armatus) is a unique economic fish species in China and exhibits significant dimorphism of male female phenotypes. Cultivating all-male seedlings can significantly improve production efficiency. To investigate sex differentiation gonadal development M. armatus, high-throughput sequencing technology was used to analyze the transcriptomes gonads at different developmental stages, both before after differentiation. We identified key genes involved differentiation, male-specific differentially expressed (DEGs), including dmrt1, amh, sox9, gsdf, dmrt2b, female-biased DEGs, foxl2, rspo1, gdf9, bmp15, wnt4. GO KEGG enrichment analyses revealed that signaling pathways such as MAPK, Wnt, TGF-β play roles armatus. expression levels 13 sex-related genes, wnt4, were determined by RT–qPCR addition RNA sequencing. RT-qPCR validation results consistent with transcriptomic data, confirming reliability our findings. This research provides valuable insights into mechanisms armatus lays foundation for developing populations aquaculture.

Language: Английский

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Differential Expression of miRNAs, lncRNAs, and circRNAs between Ovaries and Testes in Common Carp (Cyprinus carpio)

Cells, Journal Year: 2023, Volume and Issue: 12(22), P. 2631 - 2631

Published: Nov. 15, 2023

Female common carp grow faster than male individuals, implying that rearing females could be more profitable in aquaculture. Non-coding RNAs (ncRNAs) serve as versatile regulators with multiple functions diverse biological processes. However, the roles of ncRNAs sex differentiation are less studied. In this study, we investigated expression profiles ncRNAs, including miRNAs, lncRNAs, and circRNAs, gonads to comprehend carp. A substantial number differentially expressed (DE) ovaries testes were identified. Some notably miR-205, miR-214, miR-460-5p, might modulate hormone synthesis thus maintain sex. novel miRNA, novel_158, was predicted suppress foxl3. DE lncRNAs associated oocyte meiosis, GnRH signaling pathways, steroid biosynthesis, while circRNA target genes enriched WNT pathway MAPK pathway. We also analyzed ncRNA-mRNA interactions shed light on crosstalk between competing endogenous (ceRNAs), which is critical mechanism by circRNAs function. may able competitively bind novel_313, a new regulate hsd17β3. Our research will provide valuable resource for understanding genetic basis gonadal development

Language: Английский

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Histological observations and transcriptome analyses reveal the dynamic changes in the gonads of the blotched snakehead (Channa maculata) during sex differentiation and gametogenesis

Biology of Sex Differences, Journal Year: 2024, Volume and Issue: 15(1)

Published: Sept. 7, 2024

Language: Английский

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A time-course transcriptome analysis revealing the potential molecular mechanism of early gonadal differentiation in the Chinese giant salamander

Comparative Biochemistry and Physiology Part D Genomics and Proteomics, Journal Year: 2024, Volume and Issue: 50, P. 101200 - 101200

Published: Feb. 2, 2024

Language: Английский

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Analysis of Chromatin Accessibility and DNA Methylation to Reveal the Functions of Epigenetic Modifications in Cyprinus carpio Gonads

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 25(1), P. 321 - 321

Published: Dec. 25, 2023

Epigenetic modifications are critical in precisely regulating gene expression. The common carp (Cyprinus carpio) is an economically important fish species, and females exhibit faster growth rates than males. However, the studies related to epigenetic gonads limited. In this study, we conducted Assay for Transposase Accessible Chromatin sequencing (ATAC-seq) Bisulfite (BS-seq) explore roles of gonads. We identified 84,207 more accessible regions 77,922 less ovaries compared testes, some sex-biased genes showed differential chromatin accessibility their promoter regions, such as sox9a zp3. Motif enrichment analysis that transcription factors (TFs) associated with embryonic development cell proliferation were heavily enriched ovaries, TFs Foxl2 SF1 only ovaries. also analyzed possible regulations between By BS-seq, 2087 differentially methylated (promoter-DMGs) 5264 body (genebody-DMGs) CG contexts. These genebody-DMGs significantly Wnt signaling pathway, TGF-beta GnRH indicating methylation could play essential role sex maintenance, just like regions. Combined transcriptomes, revealed expression dmrtb1-like, spag6, fels was negatively correlated levels Our study on contributes elucidating molecular mechanism differentiation maintenance carp.

Language: Английский

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Effect of Larval Rearing Temperature on Steroidogenesis Pathway Development in Early Life History in Winter Flounder (Pseudopleuronectes Americanus)

Published: Jan. 1, 2024

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Language: Английский

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