Biochemical, Histological, and Multi-Omics Analyses Reveal the Molecular and Metabolic Mechanisms of Cold Stress Response in the Chinese Soft-Shelled Turtle (Pelodiscus sinensis)

Biology, Journal Year: 2025, Volume and Issue: 14(1), P. 55 - 55

Published: Jan. 11, 2025

The Chinese soft-shelled turtle (Pelodiscus sinensis), a type of warm-water reptile, is frequently chosen as the model animal to understand how organisms respond environmental stressors. However, responsive mechanism P. sinensis natural cold stress unclear, especially in terms metabolic pattern and molecular pathways. Herein, plasma biochemical, hepatic morphological, apoptotic, transcriptomic, metabolomic detection methods were performed investigate response acute stress. A consistent increase AST ALT activities with decline ALP activity was found following 14 °C 7 compared control group. Plasma GLU, TG, CHO, HDL contents, reflecting energy metabolism, decreased lower levels from 2 16 days post (dps). Histological TUNEL liver demonstrated that caused severe morphological damage cell apoptosis time-dependent manner. DEGs biosynthesis fatty acids (Acsbg2, Acsl3, Acsl4, Acsl5, Mcat, Acacb), well unsaturated (Hsd17b12, Elovl7, Scd, Baat), starch sucrose metabolism (Pgm1, Pgm2, Treh), (Ddit3, Gadd45a, Lmnb1, Tuba1c, Tnf, Tnfsf10, Fos, Itpr1, Ctso) discovered transcriptome under data showed metabolites, including chenodeoxycholic acid, oleoylethanolamide, uric fructose 1,6-bisphosphate, CMP, S-(Hydroxymethyl)-glutathione, remarkably altered groups. Combined transcriptomic revealed pyrimidine amino acid pyruvate most significant pathways regulated by low-temperature exposure. Overall, this work suggests could induce obvious at 4 dps. Moreover, main signaling for sinensis.

Language: Английский

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Long-term thermal stress induces hepatic injury and alters the thermotolerance response in Hong Kong catfish (Clarias fuscus)

Aquaculture, Journal Year: 2024, Volume and Issue: 590, P. 741041 - 741041

Published: May 6, 2024

Global climate change has a significant impact on fish survival and the aquaculture industry. Even highly adaptable organisms like Siluriformes are susceptible to these effects. This study investigates consequences of extreme high temperature Hong Kong catfish (Clarias fuscus), species known for its long-term habitation in tropical subtropical regions. In this study, C. fuscus were cultivated 90 days at (HT, 34 °C) normal (NT, 26 °C), followed by subjecting two groups stress (34 recovery (26 °C). The hepatic histology, biochemical, transcriptomic characteristics examined before acute (NT-C, HT-C), after (NT-T, HT-T), (NT-R, HT-R). histological analysis revealed that heat damaged liver tissue fuscus. Furthermore, damage was observed tissues NT group under stress, while no further HT group. biochemical showed altered body's immune oxidative balance, resulting an increase alkaline phosphatase (ALP), alanine aminotransferase (ALT), catalase (CAT), superoxide dismutase (SOD) glutathione peroxidase (GPX) activities, decrease malondialdehyde (MDA) content. Upon function disordered unable produce enough total-bilirubin (T-BIL), aspartate (AST) activity more being affected. Long-term led changes gene expression, with showing three times differentially expressed genes (DEGs) identified compared conditions. Analysis DEG enrichment during same treatment period both differences amino acid metabolism lipid pathways. These results suggest caused fuscus, altering enzyme sensitivity, as well response pathways intensity. lays groundwork future investigations into adaptability sudden strategies mitigate facing temperatures.

Language: Английский

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Liver transcriptome analysis reveal the metabolic and apoptotic responses of Trachinotus ovatus under acute cold stress

Fish & Shellfish Immunology, Journal Year: 2024, Volume and Issue: 148, P. 109476 - 109476

Published: March 4, 2024

Language: Английский

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Effects of acute flow velocity stress on oxygen consumption rate, energy metabolism and transcription level of mandarin fish (Siniperca chuatsi)

Aquaculture Reports, Journal Year: 2024, Volume and Issue: 38, P. 102293 - 102293

Published: Aug. 8, 2024

Language: Английский

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Assessing Heat Resistance and Selecting Heat-Resistant Individuals of Largemouth Bass (Micropterus salmoides) with Tiered Thermal Exposure

Animals, Journal Year: 2025, Volume and Issue: 15(2), P. 128 - 128

Published: Jan. 8, 2025

Largemouth bass (LMB, Micropterus salmoides), a commercially important farmed fish, is vulnerable to heat stress. Breeding heat-resistant LMB highly desirable in the face of global warming. However, we still lack an efficient method assess resistance LMB. In this study, critical thermal maximum (CTmax) and static exposure methods were first performed juveniles. The CTmax values experimental fish (average body weight 9.87 ± 3.14 g) ranged from 39 40 °C but too close together differentiate individual resistance. Static experiments with varying temperatures groups also did not provide clear for determining To address these limitations, developed tiered method, where temperature was increased step-wise, starting 28 34 at 2 increments then 0.5 above °C, each step lasting one day. quantified as lethal cumulative (LCT), allowing classification sensitive or resistant stress based on their LCT values. correlate changes tissue structure gene expression resistance, new batch juveniles 23.66 6.98 subjected exposure. Brain liver tissues collected control (without exposure), (still alive demonstrated abnormal symptoms) individuals when maintained 35.5 24 h. heat-sensitive showed significant damage cell apoptosis (p < 0.05) relative those ones. ddit3/chop, bax casp3 genes differential expressions fish. Additionally, 84.06 20.95 found be more than adults different sources 364.29 84.43 g 545.71 184.56 g). Through extremely successfully selected population 22.69 6.89 These findings valuable insights into biology potential breeding varieties.

Language: Английский

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Dynamics of proximate composition, fatty acid profile and transcriptome response to low-temperature stress in muscle tissues of spotted sea bass (Lateolabrax maculatus)

Aquaculture, Journal Year: 2025, Volume and Issue: 599, P. 742139 - 742139

Published: Jan. 9, 2025

Language: Английский

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High-throughput screening of thermal tolerant candidate genes in the ivory shell (Babylonia areolata) based on the yeast strain INVSc1

Comparative Biochemistry and Physiology Part D Genomics and Proteomics, Journal Year: 2025, Volume and Issue: unknown, P. 101428 - 101428

Published: Jan. 1, 2025

Language: Английский

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Mulberry leaf extract reduces damage by regulating the oxidative response, immune response and intestinal flora of largemouth bass (Micropterus salmoides) under chronic heat stress

Frontiers in Marine Science, Journal Year: 2025, Volume and Issue: 12

Published: Feb. 7, 2025

Chronic heat stress (HS) induces oxidative damage, low immunity, and intestinal flora disturbance of fish, posing great challenges to the aquaculture industry. As an important plant extract, mulberry leaf extract (MLE) has been shown have antioxidant immune-boosting properties. This study evaluated protective effect dietary MLE on HS-induced liver injury in Largemouth bass ( Micropterus salmoides ) based damage parameters, immune composition. Fish were randomly assigned into three groups: CON group (ambient temperature, 26°C, basal diet), HS (33°C, + diet supplemented with 9 g/kg MLE). significantly decreased final body weight, specific growth rate, villus length, muscular layer thickness, ACE Chao1 indices, lipase, trypsin, total superoxide dismutase (T-SOD), glutathione peroxidase (GPx), alkaline phosphatase (AKP), lysozyme (LZM) activities, but increased feed conversion aspartate aminotransferase (AST) activity, reactive oxygen species (ROS), malondialdehyde (MDA), complement 3 (C3) contents, relative abundances Proteobacteria Plesiomonas . Compared group, improved GPx, ACP, LZM indices intestine, numbers vacuoles inflammatory cells, AST GLU, MDA, ROS contents. Dietary also up-regulated mRNA expressions gpx , tg f-β il-10 jnk2 bcl-2 down-regulated il-8 tnf-α caspase-3 atf4 chop ire1, traf2 jnk1 TRPV MCU VDAC liver. In conclusion, supplementation could improve growth, diversity heat-stressed largemouth bass, alleviate hepatic injuries by regulating inflammation, stress, apoptosis, providing a theoretical basis for development as treatment against HS.

Language: Английский

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Long-term thermal stress reshapes the tolerance of head kidney of Hong Kong catfish (Clarias fuscus) to acute heat shock by regulating energy metabolism and immune response

Comparative Biochemistry and Physiology Part D Genomics and Proteomics, Journal Year: 2025, Volume and Issue: 54, P. 101437 - 101437

Published: Feb. 8, 2025

Language: Английский

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The Effects of Acute Temperature Changes on Transcriptomic Responses in the Liver of Leopard Coral Groupers (Plectropomus leopardus)

Antioxidants, Journal Year: 2025, Volume and Issue: 14(2), P. 223 - 223

Published: Feb. 15, 2025

The leopard coral grouper (Plectropomus leopardus) is a commercially significant tropical marine species. With the ongoing effects of global climate change, increasing attention has been focused on grouper's susceptibility to extreme cold weather. This study investigates acute exposure and temperature recovery liver P. leopardus. Histological observations enzyme activity assays revealed that fluctuations caused disruptions normal physiology, including lipid accumulation alterations in antioxidant levels. Transcriptomic analysis tissue identified 2744 differentially expressed genes (DEGs) across three experimental groups: 25 °C (control), 13 (cold exposure), rewarming at (R-25 °C). Functional enrichment these DEGs were significantly associated with biological processes such as metabolism defense, well pathways related metabolism, fatty acid biosynthesis, ferroptosis. Furthermore, dynamic regulation immune responses, oxidative stress was observed response both rewarming. Notably, several redox-related identified, their interactions further explored. Additionally, representative antioxidants got1, gpx1a, gpt, g6pcla.2, validated by qRT-PCR fluorescence situ hybridization (FISH). Taken together, this provides systematic grouper, laying groundwork for research responses teleost

Language: Английский

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