BMC Genomics, Journal Year: 2024, Volume and Issue: 25(1)

Published: Nov. 20, 2024

Abstract Background The short-snout icefish, Neosalanx brevirostris , a member of the Salangidae family, is an economically important fishery species in China. Understanding mechanisms underlying sex determination this has crucial implications for conservation, ecology and evolution. Meanwhile, there shortage rapid cost-effective genetic methods identification, which poses challenges identifying immature individuals mechanism studies aquaculture breeding applications. Results Based on whole genome resequencing data, sex-specific loci regions were found to be concentrated region chromosome 2. All exhibited excess heterozygosity females complete homozygosity males. This determining contains seven genes, including cytochrome P450 aromatase CYP19B involved steroidogenesis associated with 24 two W-deletions. A haploid female-specific sequence was identified as paralogous diploid significant length difference, making it suitable identification by traditional PCR agarose gel electrophoresis, further validated males known phenotypic sexes. Conclusions Our results confirm that N. exhibits female heterogametic system (ZZ/ZW), 2 putative containing relatively small (~ 48 Kb). gene proposed candidate gene. Moreover, development based method enables at any developmental stage, thereby facilitating advancing applications species.

Language: Английский