Integrated m6A RNA methylation and transcriptomic analysis of Apostichopus japonicus under combined high-temperature and hypoxia stress DOI Creative Commons
Qiang Wang, Shuqing Zhang, Xiaohua He

et al.

BMC Genomics, Journal Year: 2025, Volume and Issue: 26(1)

Published: April 10, 2025

Global climate change has significantly increased environmental stress in marine ecosystems, with rising sea surface temperatures and declining dissolved oxygen (DO) levels. These stressors pose critical challenges to aquaculture, particularly for Apostichopus japonicus, an economically significant species China. A. japonicus is highly sensitive combined high-temperature hypoxia stress, which disrupts physiological processes, suppresses immune responses, increases mortality. While epigenetic mechanisms such as N6-methyladenosine (m6A) RNA modifications are known regulate adaptation, their role under dual remains poorly understood. This study integrates m6A methylation sequencing (MeRIP-seq) transcriptomic analysis (RNA-seq) investigate molecular responses (32 °C) (DO = 2 mg/L). Results show that approximately 90% of genes had 1-3 peaks, single peaks being the most frequent (∼ 60%). Genes exhibited varying expression levels, some showing higher expression, suggesting a complex relationship between stress-responsive gene expression. GO KEGG enrichment analyses revealed m6A-modified pathways associated oxidative protein homeostasis, energy metabolism, PI3K-Akt MAPK signaling pathways. Key genes, including HSP70, NOX5, SLC7A11, dynamic changes, highlighting roles redox homeostasis cellular resilience. Comparative across experimental groups distinct hypoxia, combination, inducing more pronounced changes In this study, we explored central regulatory response hypoxia. The findings modification regulates key allowing effectively adapt harsh conditions. not only provides important new perspective on recovery mechanism invertebrates face but it also theoretical support aquaculture practice, assisting development stress-resistant systems deal severe posed by global change.

Language: Английский

Integrated m6A RNA methylation and transcriptomic analysis of Apostichopus japonicus under combined high-temperature and hypoxia stress DOI Creative Commons
Qiang Wang, Shuqing Zhang, Xiaohua He

et al.

BMC Genomics, Journal Year: 2025, Volume and Issue: 26(1)

Published: April 10, 2025

Global climate change has significantly increased environmental stress in marine ecosystems, with rising sea surface temperatures and declining dissolved oxygen (DO) levels. These stressors pose critical challenges to aquaculture, particularly for Apostichopus japonicus, an economically significant species China. A. japonicus is highly sensitive combined high-temperature hypoxia stress, which disrupts physiological processes, suppresses immune responses, increases mortality. While epigenetic mechanisms such as N6-methyladenosine (m6A) RNA modifications are known regulate adaptation, their role under dual remains poorly understood. This study integrates m6A methylation sequencing (MeRIP-seq) transcriptomic analysis (RNA-seq) investigate molecular responses (32 °C) (DO = 2 mg/L). Results show that approximately 90% of genes had 1-3 peaks, single peaks being the most frequent (∼ 60%). Genes exhibited varying expression levels, some showing higher expression, suggesting a complex relationship between stress-responsive gene expression. GO KEGG enrichment analyses revealed m6A-modified pathways associated oxidative protein homeostasis, energy metabolism, PI3K-Akt MAPK signaling pathways. Key genes, including HSP70, NOX5, SLC7A11, dynamic changes, highlighting roles redox homeostasis cellular resilience. Comparative across experimental groups distinct hypoxia, combination, inducing more pronounced changes In this study, we explored central regulatory response hypoxia. The findings modification regulates key allowing effectively adapt harsh conditions. not only provides important new perspective on recovery mechanism invertebrates face but it also theoretical support aquaculture practice, assisting development stress-resistant systems deal severe posed by global change.

Language: Английский

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