The influence of ammonia-N and salinity levels on oxidative stress markers, hepatic enzymes, and acid phosphatase activity in Nile tilapia (Oreochromis niloticus)

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Jan. 2, 2025

The point of our study was to examine the interaction ammonia-N poisoning and salinity on serum enzymes oxidative stress factors blood liver in Nile tilapia (Oreochromis niloticus). 50% lethal concentration (LC50) 96 h 0.86 mg/L ammonia-N. A random allocation used divide fish into 12 treatments. These treatments encompassed various combinations acute levels (0 LC50-96 h), sub-acute (30% (0, 4, 8, ppt). experimental design employed a factorial arrangement 3 × 4.The findings revealed that amounts aspartate transferase (AST) alanine transaminase (ALT) 4 increased significantly compared treatment 2 (4 ppt) control. Salinity did not affect glutathione (GSH), nevertheless reduction GSH total antioxidant capacity (TAC) superoxide dismutase (SOD) catalase activities (CAT) ammonia treatments, 5 9, control, states can stimulate fish. Similar measurements, increasing (5, 6, 7 8) caused an effect TAC value, which presumably due improving reducing ambient ammonia. indicate while elevated be beneficial mitigating effects toxicity water, combined presence salinity, ammonia, their had detrimental impacts physiological well-being over 96-hour testing period.

Language: Английский

---

Long term salinity disrupts the hepatic function, intestinal health, and gills antioxidative status in Nile tilapia stressed with hypoxia

Ecotoxicology and Environmental Safety, Journal Year: 2021, Volume and Issue: 220, P. 112412 - 112412

Published: June 10, 2021

In aquaculture, fish are stressed with several factors involved in impacting the growth rate and health status. Although Nile tilapia can resist brackish water conditions, hypoxia status may impair condition of fish. were exposed to salinity at 0, 10, 20‰ for four weeks then behavior was checked. The results showed meaningfully lowered rate, feed utilization, survival when kept weeks. Then subdivided into six groups (factorial design, 2 × 3) normoxia (DO, 6 mg/L) 1 conditions 24 h. High (10 20‰) combined stress-induced inflammatory features intestines, gills, livers activities SOD, CAT, GPX increased grown 10 stress. Fish had highest ALT, AST, ALP levels (p < 0.05) among groups. transcription Il-8, Il-1β, Ifn-γ, Tnf-α, Caspase-3 genes lowest level Il-10 gene observed hypoxia. outputs Integrated Biomarker Response (IBR) marked differences between varied values. IBR reared fresh normoxia, while seen group 0.05). These confirm that tolerate 10‰ but oxidative stress, apoptosis, livers. obtained indicate affect performances or high-water leading severe economic loss. Further future studies required understand impact different salinities short term long-term periods on productivity tilapia.

Language: Английский

---

Gill oxidative damage caused by acute ammonia stress was reduced through the HIF-1α/NF-κb signaling pathway in golden pompano (Trachinotus ovatus)

Ecotoxicology and Environmental Safety, Journal Year: 2021, Volume and Issue: 222, P. 112504 - 112504

Published: July 12, 2021

This study aimed to investigate the intoxication mechanism of golden pompano (Trachinotus ovatus) exposed high ammonia levels and effects on immune antioxidant mechanisms gills. Juvenile was (total ammonia: 26.9 mg/L) induce 96 h stress, a recovery experiment performed after poisoning. Then, we evaluated hematological parameters, histological structure expression related genes. In this experiment, continuous exposure led significant increase in plasma alkaline phosphatase (ALP), acid (ACP) lactate dehydrogenase (LDH) (P < 0.05), triiodothyronine (T3) tetraiodothyronine (T4) were significantly reduced 0.05). Moreover, genes such as superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX) inflammatory cytokines tumor necrosis factor α (TNF-α) interleukin 1β (IL-1β) increased These results indicate that activates active osmotic regulatory fish gills participates defense responses. However, with prolonged ammonia, balance system is disrupted, leading oxidative damage inflammation gill tissue. research not only helps elucidate by at molecular level but also provides theoretical basis for further detoxification mechanisms.

Language: Английский

---

Alterations in hematological and biochemical parameters, oxidative stress, and immune response in Takifugu rubripes under acute ammonia exposure

Comparative Biochemistry and Physiology Part C Toxicology & Pharmacology, Journal Year: 2021, Volume and Issue: 243, P. 108978 - 108978

Published: Jan. 22, 2021

Language: Английский

---

Vitamin C Attenuates Oxidative Stress, Inflammation, and Apoptosis Induced by Acute Hypoxia through the Nrf2/Keap1 Signaling Pathway in Gibel Carp (Carassius gibelio)

Antioxidants, Journal Year: 2022, Volume and Issue: 11(5), P. 935 - 935

Published: May 9, 2022

Previous studies have found that vitamin C (VC) has protective effects in fish. However, the efficacy of VC on hypoxia-induced liver injury fish remains unknown. Therefore, to investigate mechanism after acute hypoxic stimulation fish, gibel carp were fed a diet containing for eight weeks, then subjected hypoxia stimulation. The specific growth rate was increased by supplementation VC. Plasma stress markers (glucose, lactic acid, and cortisol) decreased supplementation. Moreover, levels inflammatory cytokines (tnf-α, il-2, il-6, il-12) enhancing Nrf2/Keap1 signaling pathway. Upregulation antioxidant enzymes activity (CAT, SOD, GPx); T-AOC; anti-inflammatory factors (il-4 tgf-β) highlighted activities results showed reduced apoptotic index hypothalamus. expression GRP78 protein endoplasmic reticulum apoptosis induced inhibited Taken together, indicate can attenuate oxidative damage, inflammation, via identify new defense strategy response conditions.

Language: Английский

---

Di-(2-ethylhexyl) phthalate exacerbated the toxicity of polystyrene nanoplastics through histological damage and intestinal microbiota dysbiosis in freshwater Micropterus salmoides

Water Research, Journal Year: 2022, Volume and Issue: 219, P. 118608 - 118608

Published: May 16, 2022

Language: Английский

---

A review of the toxic effects of ammonia on invertebrates in aquatic environments

Environmental Pollution, Journal Year: 2023, Volume and Issue: 336, P. 122374 - 122374

Published: Aug. 25, 2023

Language: Английский

---

Nutrient sensing signaling and metabolic responses in shrimp Litopenaeus vannamei under acute ammonia stress

Ecotoxicology and Environmental Safety, Journal Year: 2023, Volume and Issue: 253, P. 114672 - 114672

Published: Feb. 22, 2023

Ammonia is the primary environmental factor affecting growth and health of crustaceans. It would induce oxidative stress metabolic disorders. Extra amount energy was demanded to maintain physiological functions under ammonia stress. However, limited information available on its effects main nutrient metabolism, as well sensing signaling pathways. In present study, shrimp Litopenaeus vannamei were exposed acute injected with amino acid solution. The results showed that exposure resulted in lower free levels hemolymph, incomplete activation mechanistic target rapamycin (mTOR) cascaded less protein synthesis muscle. induced autophagy activated AMP-activated kinase (AMPK) pathway. Meanwhile, enhanced glycolysis lipogenesis, but inhibited lipolysis. characterized integrated responses provides critical clues understand performance

Language: Английский

---

miR-199-5p mediates the regulation of autophagy by targeting mTOR signaling and involvement in ammonia detoxification under ammonia stress in yellow catfish (Pelteobagrus fulvidraco)

Aquaculture, Journal Year: 2024, Volume and Issue: 589, P. 740977 - 740977

Published: April 24, 2024

Language: Английский

---

Effects of ammonia nitrogen stress on the physiological, biochemical, and metabolic levels of the gill tissue of juvenile four-finger threadfin (Eleutheronema tetradactylum)

Aquatic Toxicology, Journal Year: 2024, Volume and Issue: 274, P. 107049 - 107049

Published: Aug. 11, 2024

Language: Английский

---