A detailed transcriptome study uncovers the epigenetic characteristics associated with Aromatase inhibitor-induced masculinization in Takifugu rubripes larvae gonads

BMC Genomics, Journal Year: 2025, Volume and Issue: 26(1)

Published: April 16, 2025

Takifugu rubripes is an economically valuable fish species in Asia. The implementation of all-male culture for T. highly anticipated aquaculture. Aromatase inhibitor (AI, letrozole) treatment was found to be efficient method induced masculinization rubripes, as reported our previous study. Here, further explore the underlying regulation mechanism AI-induced masculinization, a whole-transcriptome analysis comparing conducted between masculinized XX (AI-XX) gonads and control (Con) rubripes. In Con-XX/Con-XY comparison, 1,172 differential expression (DE) mRNAs, 129 DEmiRNAs, 210 DElncRNAs, 4 DEcircRNAs were identified. Con-XX/AI-XX 1,329 DEmRNAs, 174 6 280 DElncRNAs found. comparisons shared 690 50 3 DEcircRNAs, 105 DElncRNAs. analyses protein-protein interaction (PPI) competitive endogenous RNA (ceRNA) network identified interactions among these DERNAs. Kcnh2b, trim27, cnnm2b, reln, cckbra, pkd1l2, steap4, gsg1l, hamp, foxg1c predicted top ten hub genes. miRNAs included miRNA-27 family miRNA-489 showed targeting relationship with GO KEGG functional enrichment that targeted genes mainly enriched GO:0065008 biological quality TGF-beta signaling pathway. qPCR validation confirmed selected ncRNAs. This research comprehensively reveals potential regulatory effects ncRNAs on cellular motility, fate regulation, hormonal during gonadal It may provide significant insights into mechanisms sex reversal fish.

Language: Английский

Analysis of gonadal transcriptome reveals core long non-coding RNA-mRNA regulatory network in sea cucumber Apostichopus japonicus

Comparative Biochemistry and Physiology Part D Genomics and Proteomics, Journal Year: 2024, Volume and Issue: 54, P. 101396 - 101396

Published: Dec. 9, 2024

Language: Английский