Fungal Biotechnology Applications in Sustainable Oil Extraction

Applied Microbiology, Journal Year: 2025, Volume and Issue: 5(1), P. 8 - 8

Published: Jan. 15, 2025

This paper examines the role of filamentous fungi in enhancing sustainable extraction vegetable oils from oilseeds. Fungi such as Aspergillus, Penicillium, Fusarium, Trichoderma, and Rhizopus are highlighted for their ability to produce hydrolytic enzymes, including lipases, cellulases, hemicellulases, which break down plant cell walls facilitate oil release. biotechnological approach not only improves yield but also reduces operational costs environmental impacts, contributing development goals. The integration oleaginous fungi, capable accumulating lipids, is discussed a promising avenue boosting production efficiency. Furthermore, this underscores importance combining traditional knowledge with modern advancements. respects local cultural practices while optimizing processes, ensuring minimal ecological disruption. use oilseed degradation represents significant step towards more eco-friendly cost-effective production, making it valuable contribution agricultural industrial practices.

Language: Английский

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Pseudomonas aeruginosa Rhamnolipids Produced by Andiroba (Carapa guianensis Aubl.) (Sapindales: Meliaceae) Biomass Waste from Amazon: A Potential Weapon Against Aedes aegypti L. (Diptera: Culicidae)

Molecules, Journal Year: 2025, Volume and Issue: 30(3), P. 618 - 618

Published: Jan. 31, 2025

Rhamnolipids, biosurfactants synthesized from natural resources, demonstrate significant applications, including notable insecticidal efficacy against Aedes aegypti L., the primary vector for numerous arboviruses. The global spread of A. poses substantial public health challenges, requiring innovative and sustainable control strategies. This research investigates use andiroba (Carapa guianensis Aubl.) biomass waste as a substrate synthesizing rhamnolipid biosurfactant (BSAW) produced by Pseudomonas aeruginosa evaluates its activity aegypti. findings indicate yield 4.42 mg mL-1, alongside an emulsification index approaching 60%. BSAW successfully reduced both surface interfacial tensions to below 30 mN/m 4 mN/m, respectively. Characterization revealed that is di-rhamnolipid, consisting two rhamnose units covalently linked saturated C10 fatty acid chain. At concentration 1.0 exhibited larvicidal activity, leading structural impairments cellular dysfunctions in larvae while also disrupting their associated bacterial microbiota. Moreover, effectively deterred oviposition adult mosquitoes. These underscore BSAW's potential compromise various developmental stages aegypti, supporting integrated arbovirus management approaches. Furthermore, this emphasizes feasibility utilizing agro-industrial substrates microbial production.

Language: Английский

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Anatomical study of the seed of andiroba (Carapa guianensis) through stereo and scanning electron microscopy

Acta Amazonica, Journal Year: 2025, Volume and Issue: 55

Published: Jan. 1, 2025

ABSTRACT The points of susceptibility seeds to deterioration are important factors, especially when stored in hot and humid tropical conditions. andiroba (Carapa guianensis) an resource for Amazonian traditional communities, as the oil extracted from is widely used popular medicine cosmetic industry. Yet subject fungal spoilage, which affects quality oil. We analyzed morpho-histological characteristics whole by stereo scanning electron microscopy identify fungi. shell (tegument), a thick lignin-rich, protective wall structure, varied type number tissue layers, with polygonal long shaped cells presenting pores (plasmodesmata). junction three faces at top formed relatively tight small cavity, micropyle, rich vascular bundles helically tracheid-shaped vessels. An interchange channel pod through micropyle inner seed between thin brown skin towards cotyledons, its surface covered adhered residues dry placental tissue. cotyledons (cross longitudinal sections), presented different cell layers containing randomly distributed lipid droplets. concluded that highly irregular top, forms deep recesses accumulates residues, presents righest risk fungi conidia deposition and/or moisture absorption, leads spoilage.

Language: Английский

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Cadeias de plantas medicinais e fitoterápicos e o potencial do guaraná e da andiroba no estado do Amazonas pela ótica dos atores locais

Revista Fitos, Journal Year: 2025, Volume and Issue: 19(1)

Published: March 1, 2025

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The Development and Characterization of an Andiroba Oil-Based Nanoemulsion (Carapa guianensis, Aubl.): Insights into Its Physico-Chemical Features and In Vitro Potential Healing Effects

Pharmaceutics, Journal Year: 2025, Volume and Issue: 17(4), P. 498 - 498

Published: April 9, 2025

Background/Objectives: Andiroba oil, extracted from Carapa guianensis seeds, possesses therapeutic properties including anti-inflammatory and wound healing effects. This study aimed to develop characterize a nanoemulsion formulation containing andiroba oil (NeAnd) evaluate its cytotoxicity potential in vitro. Methods: The was evaluated for acidity, antioxidant activity, fatty acid composition. NeAnd produced by ultrasonication characterized using FTIR (Fourier transform infrared spectroscopy), Raman spectroscopy, dynamic light scattering, transmission electron microscopy. Results: exhibited spherical shape stable physicochemical properties, with an average hydrodynamic diameter (HD) of 205.7 ± 3.9 nm, polydispersity index (PdI) 0.295 0.05, negative zeta −4.16 0.414 mV, pH approximately 6.5. These nanodroplets remained 120 days when stored at 4 °C maintained their parameters even under variations. analyses confirmed the presence functional groups organization chains NeAnd. Cell viability assays revealed no statistically significant differences various concentrations (90–360 µg/mL) after 24 48 h. In scratch assays, significantly enhanced closure (88.9%) compared PBS control (38%) free (68.6%) keratinocytes (p < 0.05). Conclusions: promising findings indicate as nanophytomedicine tissue regeneration treatments.

Language: Английский

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Establishment and Characterization of a Primary Fibroblast Cell Culture from the Amazonian Manatee (Trichechus inunguis)

Animals, Journal Year: 2024, Volume and Issue: 14(5), P. 686 - 686

Published: Feb. 22, 2024

The vulnerable status of the Amazon manatee, Trichechus inunguis, indicates need to seek measures guarantee its conservation. In this context, cultivation cells in vitro is a strategy that should at least preservation their genetic material. Thus, we established for first time primary culture Amazonian manatee fibroblasts (TINsf) from skin biopsy young male. Karyotypic analysis 3rd, 7th, and 12th passages confirmed taxonomic identity species T. inunguis (2n = 56/NF 92) indicated presents genomic stability. Gene protein expression vimentin 13th passage show predominant presence TINsf. To test cell line’s responsiveness materials demonstrate possible application culture, it was exposed andiroba seed oil (ASO), viability proliferative capacity were evaluated. ASO demonstrated toxic effects highest concentrations longest exposure times tested, reproducing results observed human cultures, indicating applicability TINsf toxicological biotechnological studies. After cryopreservation, line maintained potential, establishment new available future

Language: Английский

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Exploring enzyme biotechnology's role in green chemistry and advancements in pharmaceutical technologies

Elsevier eBooks, Journal Year: 2024, Volume and Issue: unknown, P. 465 - 495

Published: Jan. 1, 2024

Language: Английский

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Andiroba oil (Carapa guianensis): Descriptive analysis and applicability

Industrial Crops and Products, Journal Year: 2023, Volume and Issue: 202, P. 117004 - 117004

Published: June 22, 2023

Language: Английский

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Formulation and performance evaluation of emulgel platform for combined skin delivery of curcumin and propolis

Pharmaceutical Development and Technology, Journal Year: 2023, Volume and Issue: 28(6), P. 559 - 570

Published: May 22, 2023

The environment can modify the physiology and body protective function of skin. Propolis (PRP) curcumin (CUR) possess important antioxidant antimicrobial properties, they be administered in a combined way using photodynamic therapy (PDT). Emulgels control drug release due to physicochemical properties gel emulsion. They constitute good strategy for achieving an improved platform delivery PRP CUR. There are no other studies emulgels composed CUR their performance as skin healing or not PDT. This study aimed investigate effect Carbopol 934 P (C934P), 974 (C974P) polycarbophil (PC) on stability, activity, profile, ex vivo permeation retention containing Formulations C974P PC displayed stability activity. activity against Staphylococcus aureus modified (extended) release, governed mainly by non-Fickian anomalous transport. resulted delivery, allowing drugs cross stratum corneum, permeate epidermis, reaching dermis. selected candidates further prove action benefits health.

Language: Английский

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Calamus caesius (Rattan) wood: Chemical constituents, biological activities’ relative medicinal properties from Thai medicinal scriptures, and in silico antioxidant activity

Arabian Journal of Chemistry, Journal Year: 2023, Volume and Issue: 16(8), P. 104990 - 104990

Published: May 22, 2023

Calamus caesius is known as Rattan. It was found a component of many formulas from evidence-based Thai medicinal scriptures but no research about their properties. We investigated the literature review analysis textbooks for proposed biological activity relatives including antioxidant, cytotoxic, anti-inflammatory, and antimicrobial activities, chemical profiles using gas chromatography-mass spectrometry (GC–MS). In silico studies were inspected on tyrosinase NAD(P)H oxidase actions. Thirty C. with percent ratio in range 1.43 to 14.99, formula's properties are antipyretic, followed by antidiarrhea, cure abscesses related inflammation infection. Both water extracted ethanol showed high antioxidant activities all assays toxicity macrophage-like cells cancer cell lines. The slightly bactericidal better than extracted, none them inhibited against albicans. From GC–MS analysis, highest components extract 3-tert-Butylamino-acrylonitrile β-Sitosterol, respectively. Five compounds revealed both 1,3-di-tert-butylbenzene; 2,6-dimethoxyphenol; 2-propylphenol; 2,4-di-tert-butylphenol; methyl palmitate. Sterol such stigmasterol, beta-sitosterol, campesterol outstanding interaction NADPH molecular docking study. All outcomes proven that has potentially effects support health problems. Additionally, this first report scientific data wood its formula scriptures.

Language: Английский

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