Organoids-on-a-chip: microfluidic technology enables culture of organoids with enhanced tissue function and potential for disease modeling
Lito Papamichail,
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Lena Sophie Koch,
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Devin Veerman
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et al.
Frontiers in Bioengineering and Biotechnology,
Journal Year:
2025,
Volume and Issue:
13
Published: March 11, 2025
Organoids
are
stem-cell
derived
tissue
structures
mimicking
specific
structural
and
functional
characteristics
of
human
organs.
Despite
significant
advancements
in
the
field
over
last
decade,
challenges
like
limited
long-term
culture
lack
maturation
hampering
implementation
organoids
biomedical
research.
Culture
microfluidic
chips
is
being
used
to
tackle
these
through
dynamic
precise
control
organoid
microenvironment.
This
review
highlights
breakthroughs
that
have
been
made
innovative
“organoids-on-chip,”
demonstrating
how
contributed
advancing
models.
We
focus
on
incorporation
representative
for
various
tissues
into
discuss
latest
findings
multi-organoids-on-chip
approaches.
Additionally,
we
examine
current
limitations
towards
development
reproducible
organoids-on-chip
systems.
Finally,
potential
technology
both
vitro
vivo
applications.
Language: Английский
Extracranial arteriovenous malformations: towards etiology-based therapeutic management
Journal of Clinical Investigation,
Journal Year:
2025,
Volume and Issue:
135(6)
Published: March 16, 2025
Anomalies
during
angiogenesis
can
initiate
the
formation
of
arteriovenous
malformations
(AVMs),
characterized
by
aberrant
connections
between
arteries
and
veins
fast
lesional
blood
flow.
These
anomalies
manifest
anywhere
in
body,
including
brain,
they
typically
appear
at
birth
evolve
alongside
growth
individual.
Depending
on
their
location
size,
AVMs
induce
progressive
deformation,
chronic
pain,
functional
impairment,
ulceration
pose
life-threatening
risks
such
as
hemorrhage
organ
dysfunction.
The
primary
treatment
modalities
entail
surgical
intervention
or
embolization
followed
surgery.
However,
these
approaches
are
often
challenging
seldom
offer
definitive
resolution.
In
addition,
inadequately
performed
surgery
may
trigger
angiogenic
rebound,
fostering
AVM
recurrence.
Advancements
comprehending
molecular
pathways
underlying
have
sparked
interest
repurposing
targeted
therapies
initially
devised
for
cancer
treatment.
first
results
promising,
giving
new
hope
to
patients
affected
with
devastating
debilitating
lesions,
management
which
presents
major
clinical
challenges.
Language: Английский
Novel strategy to target glioblastoma-initiating cells using a braintropic adeno-associated virus carrying a miR-dependent genome-editing system
British Journal of Cancer,
Journal Year:
2025,
Volume and Issue:
unknown
Published: April 11, 2025
Language: Английский
Tailored 3D microphantoms: An essential tool for quantitative phase tomography analysis of organoids
Journal of Applied Biomedicine,
Journal Year:
2025,
Volume and Issue:
45(2), P. 247 - 257
Published: April 1, 2025
Language: Английский
Sequential Angiogenic-Osteogenic Coupling via a Spatiotemporally Graded Hydrogel Enables Vascularized Bone Organoids for Critical-Sized Calvarial Defect Reconstruction
Xu Lou,
No information about this author
Fuxiao Wang,
No information about this author
Xia Lv
No information about this author
et al.
Composites Part B Engineering,
Journal Year:
2025,
Volume and Issue:
unknown, P. 112553 - 112553
Published: April 1, 2025
Language: Английский
Organoid Vascularization: Strategies and Applications
Advanced Healthcare Materials,
Journal Year:
2025,
Volume and Issue:
unknown
Published: April 26, 2025
Abstract
Organoids
provide
3D
structures
that
replicate
native
tissues
in
biomedical
research.
The
development
of
vascular
networks
within
organoids
enables
oxygen
and
nutrient
delivery
while
facilitating
metabolic
waste
removal,
which
supports
organoid
growth
maturation.
Recent
studies
demonstrate
vascularized
models
offer
insights
into
tissue
interactions
promote
regeneration.
However,
the
current
limitations
establishing
functional
affect
growth,
viability,
clinical
translation
potential.
This
review
examines
organoids,
including
mechanisms
angiogenesis
vasculogenesis,
construction
strategies,
applications.
approaches
are
categorized
vivo
vitro
methods,
with
analysis
their
specific
advantages
limitations.
also
discusses
emerging
techniques
such
as
bioprinting
gene
editing
for
improving
vascularization
integration
organoid‐based
therapies.
Current
developments
indicate
potential
applications
modeling
human
diseases
developing
therapeutic
contributing
to
advances
translational
Language: Английский
3D Organoid Models in Cancer Immunotherapy: Bioengineering Approach for Personalized Treatment
Published: Aug. 12, 2024
Recent
advances
in
organoid
technology
have
revolutionized
cancer
biology
and
thera-peutic
interventions,
offering
personalized
immunotherapy
treatment.
Organoids,
three-dimensional
cell
cultures
derived
from
patient
tumors,
accurately
replicate
the
tumor
microenvi-ronment,
providing
unprecedented
insights
into
tumor-immune
interactions
therapeutic
re-sponses.
In
this
literature-based
study,
we
discuss
various
culture
methods
for
diverse
appli-cations
of
organoids
immunotherapy,
including
drug
screening,
treatment
strategies,
mechanistic
studies.
Additionally,
address
technological
challenges
asso-ciated
with
these
propose
potential
future
solutions
to
accelerate
development
novel
immunotherapeutic
approaches.
This
review
highlights
transformative
or-ganoid
models
advancing
preclinical
modeling,
evalua-tion,
paving
way
more
effective
treatments.
Language: Английский