Early and Late Responses of Cultured Human Mesenchymal Stem Cells (MSCs) to Cell-free DNA (cfDNA) in Patients With Acute Myocardial Infarction DOI Creative Commons
Elena M. Malinovskaya, Н. Н. Вейко, Elizaveta S. Ershova

et al.

Frontiers in Bioscience-Landmark, Journal Year: 2025, Volume and Issue: 30(4)

Published: March 31, 2025

Background: Acute myocardial infarction (AMI) is accompanied by damage to heart tissues and some cell death. Stem cells are localized in the affected area contribute tissue repair. Studies have previously shown that concentration of cell-free DNA (cfDNA) blood (ami-cfDNA) increases significantly patients with AMI, GC-rich oxidized fragments accumulate composition ami-cfDNA. As a result, ami-cfDNA exhibits biological activity vitro against various types differentiated human cells. Potentially, can influence functional direction stem differentiation. To verify this assumption, we investigated effect isolated from AMI on adipose mesenchymal (MSCs) vitro. Materials Methods: The MSC line was used characterized surface markers. Ami-cfDNA control (hc-cfDNA) samples were plasma seven ten healthy donors. early (0.5–3 hours) late (1–3 weeks) responses MSCs cfDNA action analyzed. level reactive oxygen species, expression numerous genes (NOX4, NRF2, BRCA1, BCL2, BAX, MYOD1, MYOG, MYF5, MRF4, RUNX2, SPP1, OCN, LPL, AP2), double-stranded breaks nuclei, changes spatial organization chromatin nucleus determined using quantitative (real-time) polymerase chain reaction (qPCR), flow cytometry, fluorescence microscopy, fluorescent situ hybridization (FISH) assays. Results: Introducing into culture medium stimulates rapid transient induction oxidative stress (early response). Oxidative reorganization develop an adaptive response (AR). includes antioxidant anti-apoptotic activation repair genes. fragments, unlike hc-cfDNA, stimulate myogenic differentiation under prolonged exposure (late Conclusions: survival model system inducing pronounced cellular response. Prolonged provokes MSCs. Under acute conditions caused body, may positively affect restoration damaged muscle.

Language: Английский

Epigenetic regulation in coronary artery disease: from mechanisms to emerging therapies DOI Creative Commons
Rui Gao, Meilin Liu, Haoyi Yang

et al.

Frontiers in Molecular Biosciences, Journal Year: 2025, Volume and Issue: 12

Published: Jan. 31, 2025

Atherosclerosis, the primary cause of coronary artery disease (CAD), remains a leading global mortality. It is characterized by accumulation cholesterol-rich plaques and inflammation, which narrow arteries increase risk rupture. To elucidate this complex biological process improve therapeutic strategies, CAD has been extensively explored from an epigenetic perspective over past two decades. Epigenetics field investigating heritable alterations in gene expression without DNA sequence changes, such as methylation, histone modifications, non-coding RNAs. Increasing evidence indicated that development significantly influenced changes. Meanwhile, impact epigenetics now transitioning pathophysiology to therapeutics. Focusing on key enzymes their target genes will help facilitate diagnosis treatment CAD. This review synthesizes novel insights into CAD, addressing pathological processes, molecular mechanisms, potential biomarkers. Furthermore, we discuss emerging strategies targeting pathways. By focusing pivotal associated genes, work aims advance diagnostics interventions.

Language: Английский

Citations

0
Comparison and mechanism analysis of fatty acid differences between backcross F2 derived from blunt snout bream (Megalobrama amblycephala,♀) × topmouth culter (Culter alburnus,♂) and its closely related species DOI

Qixiang Wang,

Haoyang Wangchen,

Jinhong Luo

et al.

Aquaculture International, Journal Year: 2025, Volume and Issue: 33(4)

Published: April 9, 2025

Language: Английский

Citations

0
Early and Late Responses of Cultured Human Mesenchymal Stem Cells (MSCs) to Cell-free DNA (cfDNA) in Patients With Acute Myocardial Infarction DOI Creative Commons
Elena M. Malinovskaya, Н. Н. Вейко, Elizaveta S. Ershova

et al.

Frontiers in Bioscience-Landmark, Journal Year: 2025, Volume and Issue: 30(4)

Published: March 31, 2025

Background: Acute myocardial infarction (AMI) is accompanied by damage to heart tissues and some cell death. Stem cells are localized in the affected area contribute tissue repair. Studies have previously shown that concentration of cell-free DNA (cfDNA) blood (ami-cfDNA) increases significantly patients with AMI, GC-rich oxidized fragments accumulate composition ami-cfDNA. As a result, ami-cfDNA exhibits biological activity vitro against various types differentiated human cells. Potentially, can influence functional direction stem differentiation. To verify this assumption, we investigated effect isolated from AMI on adipose mesenchymal (MSCs) vitro. Materials Methods: The MSC line was used characterized surface markers. Ami-cfDNA control (hc-cfDNA) samples were plasma seven ten healthy donors. early (0.5–3 hours) late (1–3 weeks) responses MSCs cfDNA action analyzed. level reactive oxygen species, expression numerous genes (NOX4, NRF2, BRCA1, BCL2, BAX, MYOD1, MYOG, MYF5, MRF4, RUNX2, SPP1, OCN, LPL, AP2), double-stranded breaks nuclei, changes spatial organization chromatin nucleus determined using quantitative (real-time) polymerase chain reaction (qPCR), flow cytometry, fluorescence microscopy, fluorescent situ hybridization (FISH) assays. Results: Introducing into culture medium stimulates rapid transient induction oxidative stress (early response). Oxidative reorganization develop an adaptive response (AR). includes antioxidant anti-apoptotic activation repair genes. fragments, unlike hc-cfDNA, stimulate myogenic differentiation under prolonged exposure (late Conclusions: survival model system inducing pronounced cellular response. Prolonged provokes MSCs. Under acute conditions caused body, may positively affect restoration damaged muscle.

Language: Английский

Citations

0