Construction of anticancer drug incorporated aptamer-functionalized cationic β-lactoglobulin: induction of cell cycle arrest and apoptosis in colorectal cancer DOI
Zhipeng Zhang, Tianran Zhang, Zimeng Li

et al.

Journal of Biomaterials Science Polymer Edition, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 20

Published: Oct. 16, 2024

Nanoscale drug delivery systems that are both multifunctional and targeted have been developed using proteins as a basis, thanks to their attractive biomacromolecule properties. A novel nanocarrier, aptamer (AS1411)-conjugated β-lactoglobulin/poly-l-lysine (BLG/Ap/PL) nanoparticles, was in this study. To unique formulation, the as-prepared nanocarrier blends distinctive features of an chemotherapeutic targeting agent with those protein nanocarriers. By loading cabazitaxel (CTX) onto nanocarriers, therapeutic potential BLG/Ap/PL could be demonstrated. The CTX-loaded (CTX@BLG/Ap/PL) showed regulated release profile acidic milieu, which improve efficacy cancer cells high encapsulation up 93%. However, compared free CTX, CTX@BLG/Ap/PL killed colorectal HCT116 higher at 24 48 h. Further investigation confirms apoptosis by acridine orange ethidium bromide (AO/EB), DAPI staining morphological changes, chromatin condensation, membrane blebbing treated cell through flow cytometry displayed percentages apoptosis. Cell cycle analysis revealed induced sub-G1 G2/M phase (apoptosis) Annexin V/propidium iodide (PI) confirmed induces cells. Overall, study proved had several advantages over drugs promise solution clinical problems associated antitumor systems.

Language: Английский

Application of RNA-based therapeutics in glioma: A review DOI
Mehdi Sanati, Amir R. Afshari,

Seyed Sajad Ahmadi

et al.

Progress in molecular biology and translational science, Journal Year: 2023, Volume and Issue: unknown, P. 133 - 161

Published: Dec. 29, 2023

Language: Английский

Citations

1
DNA-Based Hydrogel in Drug Delivery DOI

Pallabi Paul,

Himadree Das,

Himali Horo

et al.

Published: Jan. 1, 2024

DNA is perhaps the most important biopolymer that stores genetic information and an integrated part of living cells. Due to its high water solubility, H-bondings, pi-pi stacking interactions, offers enormous opportunities fabricate new supramolecular architectures through self-assembly. DNA-based hydrogels are highly biocompatible cargos could be engineered into novel delivery vehicles for small molecules, proteins/peptides, nucleic acids. This chapter deliberates on synthesis formulations hydrogels, fabrication biomaterials chemical modifications, various mechanisms systems. The also reviews details applications future scopes.

Language: Английский

Citations

0
Construction of anticancer drug incorporated aptamer-functionalized cationic β-lactoglobulin: induction of cell cycle arrest and apoptosis in colorectal cancer DOI
Zhipeng Zhang, Tianran Zhang, Zimeng Li

et al.

Journal of Biomaterials Science Polymer Edition, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 20

Published: Oct. 16, 2024

Nanoscale drug delivery systems that are both multifunctional and targeted have been developed using proteins as a basis, thanks to their attractive biomacromolecule properties. A novel nanocarrier, aptamer (AS1411)-conjugated β-lactoglobulin/poly-l-lysine (BLG/Ap/PL) nanoparticles, was in this study. To unique formulation, the as-prepared nanocarrier blends distinctive features of an chemotherapeutic targeting agent with those protein nanocarriers. By loading cabazitaxel (CTX) onto nanocarriers, therapeutic potential BLG/Ap/PL could be demonstrated. The CTX-loaded (CTX@BLG/Ap/PL) showed regulated release profile acidic milieu, which improve efficacy cancer cells high encapsulation up 93%. However, compared free CTX, CTX@BLG/Ap/PL killed colorectal HCT116 higher at 24 48 h. Further investigation confirms apoptosis by acridine orange ethidium bromide (AO/EB), DAPI staining morphological changes, chromatin condensation, membrane blebbing treated cell through flow cytometry displayed percentages apoptosis. Cell cycle analysis revealed induced sub-G1 G2/M phase (apoptosis) Annexin V/propidium iodide (PI) confirmed induces cells. Overall, study proved had several advantages over drugs promise solution clinical problems associated antitumor systems.

Language: Английский

Citations

0