The Gut Microbiota Metabolite Butyrate Modulates Acute Stress-Induced Ferroptosis in the Prefrontal Cortex via the Gut–Brain Axis

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(4), P. 1698 - 1698

Published: Feb. 17, 2025

Stress has been implicated in the onset of mental disorders such as depression, with prefrontal cortex (PFC) playing a crucial role. However, underlying mechanisms remain to be fully elucidated. Metabolites secreted by intestinal flora can enter bloodstream and exert regulatory effects on body. Consequently, this study aims investigate molecular which gut influences ferroptosis PFC neurons, thereby affecting depression-like behavioral changes mice subjected acute stress. Initially, we established mouse model restraint stress (3-day duration) verified that stress-induced neurons contributed alterations mice, evidenced morphological, behavioral, biology assessments. Subsequently, through fecal microbiota transplantation (FMT) experiments, significant correlation between stress-exposed mice. 16S rDNA sequencing identified butyric acid-producing bacteria, specifically g_Butyricimonas its primary metabolite, acid, critical regulators acutely stressed Furthermore, intervention butyrate demonstrated potential ameliorate damage blood-brain barriers these This also mitigated behaviors induced alleviating systemic inflammatory responses. The findings indicate plays role Additionally, metabolite modulate gut-brain axis.

Language: Английский

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The Role of Necroptosis, Pyroptosis, and Ferroptosis in Porcine Intestinal Injury and Their Regulation by Nutrients and Bioactive Substances

Journal of Nutrition, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 1, 2025

Language: Английский

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Dictamnine alleviates DSS-induced colitis mice by inhibiting ferroptosis of enterocytes via activating Nrf2-Gpx4 signaling pathway

European Journal of Pharmacology, Journal Year: 2025, Volume and Issue: unknown, P. 177464 - 177464

Published: March 1, 2025

Language: Английский

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Absolute Quantitative Metagenomic Analysis Provides More Accurate Insights for the Anti-Colitis Effect of Berberine via Modulation of Gut Microbiota

Biomolecules, Journal Year: 2025, Volume and Issue: 15(3), P. 400 - 400

Published: March 11, 2025

Current gut microbiota studies often rely on relative quantitative sequencing. However, under certain circumstances, while the abundance of these bacteria may remain stable, absolute quantities specific can vary considerably. Since function is directly linked to their total numbers, quantification crucial. This study aims identify optimal method for microbiome analysis by comparing and Using ulcerative colitis, which closely associated with microbiota, as a disease model berberine (which affects microbiota) versus sodium butyrate does not) drugs, methods were used evaluate varying effects different drugs regulation in UC-affected animals. The regulatory BBR further synthesized identified earlier using an individual-based meta-analysis, we compared findings our sequencing results. results from more consistent actual microbial community, suggesting that measurements might not accurately reflect true species. Moreover, meta-analysis only partially sometimes opposed, analyses are prone misinterpretation incorrect correlation underscores importance representing counts sample evaluating modulatory microbiome, plays vital role microbiome.

Language: Английский

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Enzymatic Oxidants, Antioxidants, and Inflammatory Bowel Disease

Applied Biosciences, Journal Year: 2025, Volume and Issue: 4(2), P. 19 - 19

Published: April 1, 2025

The role of oxidants and antioxidants in inflammatory bowel disease (IBD) has been actively explored since the early 1980s, starting with respiratory burst neutrophils ischemia pathology. Since that time, enzymatic components contributing to pool reactive oxygen species, including superoxide, H2O2, lipid hydroperoxides, counteracting antioxidants—catalase, glutathione peroxidases (Gpx), peroxiredoxins (PRDX), superoxide dismutases, others—have fleshed out. My perspective on IBD is from balance or imbalance oxidant sources process. I will present evidence involvement antioxidant processes based, as much possible, my experiences Gpxs. This be discussed terms both immune system local systems. As Gpxs are generally selenium-dependent, possible deficiencies selenium uptake active impact Gpx expression explored. more recently introduced ferroptosis, an iron-dependent peroxidation-based pathological process, reviewed for its IBD.

Language: Английский

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Sodium butyrate attenuates liver fibrogenesis via promoting H4K8 crotonylation

Molecular and Cellular Biochemistry, Journal Year: 2025, Volume and Issue: unknown

Published: April 3, 2025

Language: Английский

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The metabolites of gut microbiota: their role in ferroptosis in inflammatory bowel disease

European journal of medical research, Journal Year: 2025, Volume and Issue: 30(1)

Published: April 7, 2025

Inflammatory bowel disease (IBD) includes chronic inflammatory conditions, such as Crohn's and ulcerative colitis, characterized by impaired function of the intestinal mucosal epithelial barrier. In recent years, ferroptosis, a novel form cell death, has been confirmed to be involved in pathological process IBD is related various changes, oxidative stress inflammation. Recent studies have further revealed complex interactions between microbiome indicating that ferroptosis an important target for regulation gut microbiota its metabolites. This article reviews significant roles microbial metabolites, short-chain fatty acids, tryptophan, bile IBD. These metabolites participate influencing microenvironment, modulating immune responses, altering levels, thereby exerting impact on development Treatments based are gradually becoming research hotspot. Finally, we discuss potential current therapeutic approaches, including antibiotics, probiotics, prebiotics, fecal transplantation, microbiota, affecting improving symptoms. With deeper understanding interaction mechanisms it expected more precise effective treatment strategies will developed future.

Language: Английский

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Hericium erinaceus extract attenuates gastric mucosal injury via Nrf2/HO-1 pathway activation and gut microbiota modulation in rats

Journal of Functional Foods, Journal Year: 2025, Volume and Issue: 128, P. 106842 - 106842

Published: April 25, 2025

Language: Английский

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Glutathione Peroxidase 4 in Blunt Snout Bream (Megalobrama amblycephala) Regulates Ferroptosis and Inflammation in Response to Aeromonas hydrophila Infection

Current Issues in Molecular Biology, Journal Year: 2025, Volume and Issue: 47(5), P. 326 - 326

Published: May 2, 2025

Glutathione peroxidase 4 (GPX4) plays a crucial role in regulating lipid peroxidation and is associated with infection inflammation, particularly terms of its effects on inflammatory cytokines ferroptosis. This study aimed to investigate the regulatory Gpx4 response ferroptosis blunt snout bream (Megalobrama amblycephala), significant freshwater fish species China, after Aeromonas hydrophila infection. Using bioinformatics analysis, we discovered that has conserved protein structure high amino acid identity various carp species, indicating functional conservation across through involution. RT-qPCR analysis revealed gpx4 mRNA increased neuroembryonic stage during early development was highly expressed liver healthy adult fish. Upon A. infection, expression decreased significantly rapidly liver. In L8824 cells, overexpression suppressed inhibited both induction ferroptosis-inducer RSL3. These findings highlight cellular providing insights into complex mechanisms disease resistance potentially aiding strategies for control

Language: Английский

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Microbiota basis of antidepressant effects of Zhi-zi-chi Decoction: Lactobacillus rhamnosus and gut microbiota metabolism

Chinese Herbal Medicines, Journal Year: 2025, Volume and Issue: unknown

Published: May 1, 2025

Language: Английский

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