Cyanidin-3-O-Glucoside Mitigates Amyloid-Beta (1–42)-Induced Apoptosis in SH-SY5Y Cells by Regulating Ca2+ Homeostasis and Inhibiting Mitochondrial Dysfunction DOI Creative Commons
Chao Ma, Yu Nie, Donglei Zhang

et al.

Antioxidants, Journal Year: 2025, Volume and Issue: 14(4), P. 490 - 490

Published: April 18, 2025

Background: Blueberry anthocyanin such as Cyanidin-3-O-glucoside may help prevent Alzheimer’s disease. We aimed to investigate the preventive and therapeutic effects of against Aβ1–42-induced apoptosis SH-SY5Y cells well underlying mechanisms. Methods: Cell viability intracellular mitochondrial reactive oxygen species were detected by MTT, a detection kit, MitoSOX red superoxide indicator. The membrane potential, calcium ion content, adenotriphophate (ATP) identified via potential ATP was flow cytometry. Transcription apoptosis-related genes using real-time fluorescence quantitative polymerase chain reaction, expression proteins Western blot. Results: found that could downregulate cytochrome c, caspase 9, 3, other proteins, which consequently reduced rate apoptosis. Additionally, it upregulate Bcl-2 gene protein expression, Bax regulate permeability calcium-release channels, reduce influx into mitochondria, maintain levels, levels increase at normal level, functioning, Discussion: Taken together, showed dose-dependent cells. Conclusions: Cyanidin 3-O-glucoside better effect than in

Language: Английский

Calcium signaling is a universal carbon source signal transducer and effects an ionic memory of past carbon sources DOI Creative Commons

Kobi J. Simpson-Lavy,

Martin Kupiec

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 12, 2025

Summary Glucose is the preferred carbon source for most cells. However, cells may encounter other sources that can be utilized. How match their metabolic gene expression to source, beyond a general glucose repressive system (catabolite repression), remains little understood. By studying effect of up seven different on Snf1 phosphorylation and downstream regulated genes, we searched mechanism identifies sources. We found glycolysis metabolites glucose-6-phosphate (G6P) glucose-1-phosphate (G1P) play central role in adaptation The ratio G1P G6P activates an analogue calcium signaling via proton-exporter Pma1, regulate genes. pathway bifurcates with calcineurin reducing ADH2 (alcohol dehydrogenase) Cmk1 increasing ZWF1 (glucose-6-phosphate expression. Furthermore, not only by present source; it also past were able manipulate this ionic memory obtain high media containing galactose. Our findings provide universal which respond all

Language: Английский

Citations

0
A deadly taste: linking bitter taste receptors and apoptosis DOI Creative Commons
Zoey A. Miller, Ryan M. Carey, Robert J. Lee

et al.

APOPTOSIS, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 20, 2025

Humans can perceive five canonical tastes: salty, sour, umami, sweet, and bitter. These tastes are transmitted through the activation of ion channels receptors. Bitter taste receptors (Taste Family 2 Receptors; T2Rs) a sub-family 25 G-protein coupled receptor (GPCR) isoforms that were first identified in type II bud cells. T2Rs activated by broad array bitter agonists, which cause an increase intracellular calcium (Ca2+) decrease cyclic adenosine 3',5'-monophosphate (cAMP). Interestingly, expressed beyond oral cavity, where they play diverse non-taste roles cell physiology disease. Here, we summarize literature explores role apoptosis. Activation with agonists induces apoptosis several cancers, airway epithelia, smooth muscle, more. In many these tissues, T2R causes mitochondrial Ca2+ overload, main driver This response may be result cellular localization, nuclear mobilization and/or remnant established immunological other types. T2R-induced could pharmacologically leveraged to treat diseases altered proliferation. Future work must explore additional extra-oral T2R-expressing tissues for apoptotic responses, develop methods in-vivo studies, discover high affinity clinical application.

Language: Английский

Citations

0
Calcium Signaling Is a Universal Carbon Source Signal Transducer and Effects an Ionic Memory of Past Carbon Sources DOI Open Access

Kobi J. Simpson-Lavy,

Martin Kupiec

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(5), P. 2198 - 2198

Published: Feb. 28, 2025

Glucose is the preferred carbon source for most cells. However, cells may encounter other sources that can be utilized. How match their metabolic gene expression to source, beyond a general glucose repressive system (catabolite repression), remains little understood. By studying effect of up seven different on Snf1 phosphorylation and downstream regulated genes, we searched mechanism identifies sources. We found glycolysis metabolites glucose-6-phosphate (G6P) glucose-1-phosphate (G1P) play central role in adaptation The ratio G1P G6P activates analogue calcium signaling via proton-exporter Pma1 regulate genes. pathway bifurcates with calcineurin-reducing ADH2 (alcohol dehydrogenase) Cmk1-increasing ZWF1 (glucose-6-phosphate expression. Furthermore, not only by present source; it also past were able manipulate this ionic memory obtain high media containing galactose. Our findings provide universal which respond all

Language: Английский

Citations

0
Cyanidin-3-O-Glucoside Mitigates Amyloid-Beta (1–42)-Induced Apoptosis in SH-SY5Y Cells by Regulating Ca2+ Homeostasis and Inhibiting Mitochondrial Dysfunction DOI Creative Commons
Chao Ma, Yu Nie, Donglei Zhang

et al.

Antioxidants, Journal Year: 2025, Volume and Issue: 14(4), P. 490 - 490

Published: April 18, 2025

Background: Blueberry anthocyanin such as Cyanidin-3-O-glucoside may help prevent Alzheimer’s disease. We aimed to investigate the preventive and therapeutic effects of against Aβ1–42-induced apoptosis SH-SY5Y cells well underlying mechanisms. Methods: Cell viability intracellular mitochondrial reactive oxygen species were detected by MTT, a detection kit, MitoSOX red superoxide indicator. The membrane potential, calcium ion content, adenotriphophate (ATP) identified via potential ATP was flow cytometry. Transcription apoptosis-related genes using real-time fluorescence quantitative polymerase chain reaction, expression proteins Western blot. Results: found that could downregulate cytochrome c, caspase 9, 3, other proteins, which consequently reduced rate apoptosis. Additionally, it upregulate Bcl-2 gene protein expression, Bax regulate permeability calcium-release channels, reduce influx into mitochondria, maintain levels, levels increase at normal level, functioning, Discussion: Taken together, showed dose-dependent cells. Conclusions: Cyanidin 3-O-glucoside better effect than in

Language: Английский

Citations

0