Cobalt Protoporphyrin Downregulates Hyperglycemia-Induced Inflammation and Enhances Mitochondrial Respiration in Retinal Pigment Epithelial Cells DOI Creative Commons

Peng-Hsiang Fang,

Tzu‐Yu Lin,

Chiu-Chen Huang

et al.

Antioxidants, Journal Year: 2025, Volume and Issue: 14(1), P. 92 - 92

Published: Jan. 15, 2025

Diabetic retinopathy is characterized by hyperglycemic retinal pigment epithelial cells that secrete excessive pro-inflammatory cytokines and VEGF, leading to damage vision loss. Cobalt protoporphyrin (CoPP) a compound can reduce inflammatory responses inducing high levels of HO-1. In the present study, therapeutic effects CoPP were examined in ARPE-19 under hyperglycemia. incubated culture media containing either 5.5 mM (NG) or 25 (HG) glucose, with without addition 0.1 µM CoPP. Protein expressions samples determined Western blotting immunostaining. A Seahorse metabolic analyzer was used assess impact treatment on mitochondrial respiration NG HG media. cultured displayed different cell morphology than those induced HO-1 significantly enhanced viability Moreover, downregulated apoptotic markers upregulated APRPE-19 The also cells.

Language: Английский

Cobalt Protoporphyrin Downregulates Hyperglycemia-Induced Inflammation and Enhances Mitochondrial Respiration in Retinal Pigment Epithelial Cells DOI Creative Commons

Peng-Hsiang Fang,

Tzu‐Yu Lin,

Chiu-Chen Huang

et al.

Antioxidants, Journal Year: 2025, Volume and Issue: 14(1), P. 92 - 92

Published: Jan. 15, 2025

Diabetic retinopathy is characterized by hyperglycemic retinal pigment epithelial cells that secrete excessive pro-inflammatory cytokines and VEGF, leading to damage vision loss. Cobalt protoporphyrin (CoPP) a compound can reduce inflammatory responses inducing high levels of HO-1. In the present study, therapeutic effects CoPP were examined in ARPE-19 under hyperglycemia. incubated culture media containing either 5.5 mM (NG) or 25 (HG) glucose, with without addition 0.1 µM CoPP. Protein expressions samples determined Western blotting immunostaining. A Seahorse metabolic analyzer was used assess impact treatment on mitochondrial respiration NG HG media. cultured displayed different cell morphology than those induced HO-1 significantly enhanced viability Moreover, downregulated apoptotic markers upregulated APRPE-19 The also cells.

Language: Английский

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