Cited by Deciphering a proliferation-essential gene signature based on CRISPR-Cas9 screening to predict prognosis and characterize the immune microenvironment in HNSCC

ADORA2B promotes proliferation and migration in head and neck squamous cell carcinoma and is associated with immune infiltration DOI

Pian Li,

Kai Pang,

S. Chen

et al.

BMC Cancer, Journal Year: 2025, Volume and Issue: 25(1)

Published: April 12, 2025

Adenosine A2B receptor (ADORA2B), a G protein-coupled receptor, is implicated in tumor progression and immune regulation various cancers. However, its specific role head neck squamous cell carcinoma (HNSC) remains largely unexplored. This study aims to elucidate the expression profile, prognostic value, modulatory role, therapeutic potential of ADORA2B HNSC. Comprehensive bioinformatics analyses were performed using TCGA GEO datasets evaluate expression, clinical correlations, significance Weighted gene co-expression network analysis (WGCNA) functional enrichment conducted explore ADORA2B-associated pathways. Immune infiltration was assessed via ESTIMATE single-sample set (ssGSEA). checkpoint blockade (ICB) therapy sensitivity drug analyzed IMvigor210 NCI-60 databases, respectively. In vitro experiments, including siRNA-mediated knockdown, CCK-8 assays, colony formation, wound healing validate oncogenic ADORA2B. significantly overexpressed HNSC tissues compared adjacent normal tissues, correlated with advanced stage as well poor overall survival (OS) progression-free (PFS). Functional revealed significant downregulation immune-related pathways high groups. High associated more immunosuppressive microenvironment (TME), characterized by lower stromal scores reduced infiltration. Immunotherapy response demonstrated that patients exhibited poorer outcomes following ICB therapy. Drug identified several agents, Ixazomib citrate, Masitinib, others, candidates for patients. experiments confirmed knockdown inhibited proliferation, migration, underscoring critical progression. key driver HNSC, contributing an TME. Its prognosis immunotherapy efficacy. Targeting may enhance overcome treatment resistance, highlighting diagnostic, prognostic, biomarker.

Language: Английский

Citations

Deciphering a proliferation-essential gene signature based on CRISPR-Cas9 screening to predict prognosis and characterize the immune microenvironment in HNSCC DOI