Aster-B Modulates Oxidative Stress Responses and Carotenoid Distribution in ARPE-19 Cells DOI Creative Commons

Vidya Gopakumar,

Johannes von Lintig

Antioxidants, Journal Year: 2025, Volume and Issue: 14(5), P. 575 - 575

Published: May 10, 2025

Lipid metabolism and oxidative stress are major contributors to ocular diseases, including drusen formation photoreceptor damage. Aster-B, encoded by GRAMD1B, mediates the non-vesicular transport of cholesterol carotenoids is highly expressed in human eye, though its specific functions remain unknown. We investigated Aster-B’s role ARPE-19 cells, a model retinal pigment epithelium (RPE), using CRISPR/dCas9 generate an Aster-B-expressing cell line. Aster-B expression significantly improved survival under induced hydrogen peroxide (H2O2) was associated with activation p53 TGFβ signaling pathways, indicating modulating responses. To confirm lipid activity, we treated cholesterol-depleted cells tracked their localization. In accumulated mitochondria, while control they remained other cellular compartments. Under stress, mitochondrial carotenoid levels declined but not cells. Interestingly, enhanced exposed H2O2 had detrimental effect suggesting that function context location dependent. These findings highlight coordinating responses RPE, implications for stress-related eye diseases.

Language: Английский

Aster-B Modulates Oxidative Stress Responses and Carotenoid Distribution in ARPE-19 Cells DOI Creative Commons

Vidya Gopakumar,

Johannes von Lintig

Antioxidants, Journal Year: 2025, Volume and Issue: 14(5), P. 575 - 575

Published: May 10, 2025

Lipid metabolism and oxidative stress are major contributors to ocular diseases, including drusen formation photoreceptor damage. Aster-B, encoded by GRAMD1B, mediates the non-vesicular transport of cholesterol carotenoids is highly expressed in human eye, though its specific functions remain unknown. We investigated Aster-B’s role ARPE-19 cells, a model retinal pigment epithelium (RPE), using CRISPR/dCas9 generate an Aster-B-expressing cell line. Aster-B expression significantly improved survival under induced hydrogen peroxide (H2O2) was associated with activation p53 TGFβ signaling pathways, indicating modulating responses. To confirm lipid activity, we treated cholesterol-depleted cells tracked their localization. In accumulated mitochondria, while control they remained other cellular compartments. Under stress, mitochondrial carotenoid levels declined but not cells. Interestingly, enhanced exposed H2O2 had detrimental effect suggesting that function context location dependent. These findings highlight coordinating responses RPE, implications for stress-related eye diseases.

Language: Английский

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