Journal of Biomedical Informatics, Journal Year: 2024, Volume and Issue: unknown, P. 104729 - 104729
Published: Sept. 1, 2024
Language: Английский
Medical Oncology, Journal Year: 2025, Volume and Issue: 42(2)
Published: Jan. 18, 2025
Language: Английский
Citations
2
Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15
Published: May 20, 2024
Background: Alternol is a small molecular compound isolated from the fermentation of mutant fungus obtained Taxus brevifolia bark. Our previous studies showed that treatment induced reactive oxygen species (ROS)-dependent immunogenic cell death. This study conducted comprehensive investigation to explore mechanisms involved in Alternol-induced Methods: Prostate cancer PC-3, C4-2, and 22RV1 were used this study. interaction with heat shock proteins (HSP) was determined using CETSA assay. Alternol-regulated ER stress assessed Western blot Extracellular adenosine triphosphate (ATP) measured ATPlite Luminescence Assay System. Results: results interacted multiple cellular chaperone increased their expression levels, including endoplasmic reticulum (ER) hypoxia up-regulated 1 (HYOU1) protein 90 alpha family class B member (HSP90AB1), as well cytosolic A 8 (HSPA8). These data represented potential cause unfolded response (UPR) after treatment. Further revealed triggered ROS-dependent responses via R-like kinase (PERK), inositol-requiring enzyme 1α (IRE1α). The double-stranded RNA-dependent (PKR) but not activating transcription factor 6 (ATF6) cascades, leading ATF-3/ATF-4 activation, C/EBP-homologous (CHOP) overexpression, X-box binding XBP1 splicing induction. In addition, inhibition these cascades blunted extracellular release, one classical hallmarks Conclusion: Taken together, our demonstrate
Language: Английский
Citations
4
Cellular Signalling, Journal Year: 2025, Volume and Issue: 131, P. 111750 - 111750
Published: March 16, 2025
Prostate cancer is the most common and remains a leading cause of cancer-related deaths among men worldwide. Androgen deprivation therapy continues to be cornerstone treatment for prostate cancer. However, efficacy this treatments often limited, emergence drug resistance tumor recurrence. TAK-901, an inhibitor Aurora kinase B, has been shown inhibit growth both in vitro vivo models. To date, effect TAK-901 on underlying mechanism remain unknown. In study, we found that could proliferation, colony formation migration, while also inducing apoptosis cells. We further demonstrated activates CHK1 signaling pathway, G2/M-phase arrest these Additionally, identified EPHA2 as novel therapeutic target TAK-901. By mutating binding sites between discovered mutations reverse anti-proliferative effects Our study first reveal induces cells inhibits cell by targeting EPHA2. These findings provide valuable insights into mechanisms may develop its applications
Language: Английский
Citations
0
Food & Function, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 1, 2025
Egg yolk phospholipids are primarily composed of various fatty acids, lysophosphatidylcholine and lysophosphatidylethanolamine. Existing studies have revealed the neuroprotective activity egg phospholipids. However, it is not clear which digestion products exert activity. The objective this study was to investigate effects different structural components in based on a DMNQ-induced oxidative damage HT22 cells. findings demonstrated that pre-treatment with diverse phospholipid components, particularly polyunsaturated markedly elevated cell viability superoxide dismutase activity, diminished ROS generation, reduced malondialdehyde levels mitochondrial membrane potential. Furthermore, RNA-Seq analysis unsaturated acids its by upregulating genes involved proliferation (Jag2 Ypel3), nervous system development (Ntf5), DNA repair (H2ax), other related processes. These provide theoretical basis for future characteristic structure profound effects.
Language: Английский
Citations
0
Biomolecules, Journal Year: 2024, Volume and Issue: 14(8), P. 901 - 901
Published: July 25, 2024
Mastitis typically arises from bacterial invasion, where host cell apoptosis significantly contributes to the inflammatory response. Gram-positive bacteria predominantly utilize virulence factor lipoteichoic acid (LTA), which frequently leads chronic breast infections, thereby impacting dairy production and animal husbandry adversely. This study employed LTA develop models of mastitis in cow mammary gland cells mice. Transcriptomic analysis identified 120 mRNAs associated with endocytosis pathways that were enriched LTA-induced inflammation Mammary Alveolar Cells-large T antigen (MAC-T), numerous differential proteins also concentrated pathway. Notably, actin-related protein 2/3 complex subunit 3 (ARPC3), 4 (ARPC4), heat shock 70 (HSP70) are closely related. STRING revealed interactions among ARPC3, ARPC4, HSP70 components Histological molecular biological assessments confirmed mainly localized membrane epithelial cells. ARPC3 ARPC4 implicated mechanisms invasion initiation inflammation. Compared control group, expression levels these markedly increased, alongside significant upregulation apoptosis-related factors. While appears inhibit alleviate inflammation, its presents novel research opportunities. In conclusion, we deduced development mechanism laying foundation for further exploring interaction between (ARP2/3) HSP70.
Language: Английский
Citations
3
Journal of Biomedical Informatics, Journal Year: 2024, Volume and Issue: unknown, P. 104729 - 104729
Published: Sept. 1, 2024
Language: Английский
Citations
1