Pharmacodynamic Evaluation of Phage Therapy in Ameliorating ETEC-Induced Diarrhea in Mice Models DOI Creative Commons

Yangjing Xiong,

Lu Xia, Yumin Zhang

et al.

Microorganisms, Journal Year: 2024, Volume and Issue: 12(12), P. 2532 - 2532

Published: Dec. 8, 2024

Enterotoxigenic Escherichia coli (ETEC) is a major pathogen causing diarrhea in humans and animals, with increasing antimicrobial resistance posing growing challenge recent years. Lytic bacteriophages (phages) offer targeted environmentally sustainable approach to combating bacterial infections, particularly eliminating drug-resistant strains. In this study, ETEC strains were utilized as indicators, stable, high-efficiency phage, designated vB_EcoM_JE01 (JE01), was isolated from pig farm manure. The genome of JE01 dsDNA molecule, measuring 168.9 kb, transmission electron microscope revealed its characteristic T4-like Myoviridae morphology. effectively lysed multi-drug-resistant isolates. Stability assays demonstrated that retained activity across temperature range 20 °C 50 pH 3–11, showing resilience ultraviolet radiation chloroform exposure. Furthermore, suppressed adhesion porcine intestinal epithelial cells (IPEC-J2), mitigating the inflammatory response triggered by ETEC. To investigate vivo antibacterial efficacy phage preparations, model established using germ-free mice infected strain. findings indicated 12 h post-ETEC inoculation, intragastric administration significantly reduced mortality, alleviated gastrointestinal lesions, decreased colonization jejunum, expression cytokines IL-6 IL-8. These results demonstrate therapeutic benefit treating ETEC-induced mice. Additionally, fluorescent incorporating red protein (RFP) engineered, pharmacokinetics therapy preliminarily assessed through fluorescence imaging showed localized jejunum rapidly, within 45 min. Moreover, markedly slowed presence target gut, suggesting sustained bacteriolytic ETEC-infected intestine. conclusion, study establishes foundation for development phage-based therapies against

Language: Английский

Pharmacodynamic Evaluation of Phage Therapy in Ameliorating ETEC-Induced Diarrhea in Mice Models DOI Creative Commons

Yangjing Xiong,

Lu Xia, Yumin Zhang

et al.

Microorganisms, Journal Year: 2024, Volume and Issue: 12(12), P. 2532 - 2532

Published: Dec. 8, 2024

Enterotoxigenic Escherichia coli (ETEC) is a major pathogen causing diarrhea in humans and animals, with increasing antimicrobial resistance posing growing challenge recent years. Lytic bacteriophages (phages) offer targeted environmentally sustainable approach to combating bacterial infections, particularly eliminating drug-resistant strains. In this study, ETEC strains were utilized as indicators, stable, high-efficiency phage, designated vB_EcoM_JE01 (JE01), was isolated from pig farm manure. The genome of JE01 dsDNA molecule, measuring 168.9 kb, transmission electron microscope revealed its characteristic T4-like Myoviridae morphology. effectively lysed multi-drug-resistant isolates. Stability assays demonstrated that retained activity across temperature range 20 °C 50 pH 3–11, showing resilience ultraviolet radiation chloroform exposure. Furthermore, suppressed adhesion porcine intestinal epithelial cells (IPEC-J2), mitigating the inflammatory response triggered by ETEC. To investigate vivo antibacterial efficacy phage preparations, model established using germ-free mice infected strain. findings indicated 12 h post-ETEC inoculation, intragastric administration significantly reduced mortality, alleviated gastrointestinal lesions, decreased colonization jejunum, expression cytokines IL-6 IL-8. These results demonstrate therapeutic benefit treating ETEC-induced mice. Additionally, fluorescent incorporating red protein (RFP) engineered, pharmacokinetics therapy preliminarily assessed through fluorescence imaging showed localized jejunum rapidly, within 45 min. Moreover, markedly slowed presence target gut, suggesting sustained bacteriolytic ETEC-infected intestine. conclusion, study establishes foundation for development phage-based therapies against

Language: Английский

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