Seventy years of peroxisome research: current advances and future perspectives

Histochemistry and Cell Biology, Journal Year: 2025, Volume and Issue: 163(1)

Published: Jan. 18, 2025

Language: Английский

Acyl-coA binding protein AcbdA regulates peroxisome hitchhiking on early endosomes

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: April 23, 2025

Motor-driven transport on microtubules is critical for distributing organelles throughout the cell. Most commonly, organelle movement mediated by cargo adaptors, proteins surface of an that directly recruit microtubule-based motors. An alternative mechanism called hitchhiking was recently discovered: some move, not recruiting motors directly, but instead using membrane contact sites to attach motor-driven vesicles and hitchhike along microtubules. Organelle observed across fungi animals. In filamentous fungi, nearly all peroxisomes move early endosomes (EEs). fungus Aspergillus nidulans , EE-associated linker PxdA DipA are establishing EE-peroxisome required peroxisome movement. How recognize this subset EEs what peroxisome-membrane exist can interact with known. Here, we undertook a forward mutagenesis screen identify such proteins. We discovered acyl-coA binding (ACB) domain-containing protein AcbdA/AN1062 localizes via its tail-anchored transmembrane domain (TMD). Deleting AcbdA gene or only N-terminal ACB perturbs distribution peroxisomes. Importantly, recruitment EEs. Fatty acid (FA)-induced increases in require AcbdA, suggesting coupled FA metabolism. Mutating conserved FFAT motif, predicted endoplasmic reticulum (ER), has no effect Taken together, our data indicate tether during hitchhiking. AcbdA’s involvement peroxisome-EE site formation represents divergence from known functions Acbd4/5 adds layers understanding functionality family

Language: Английский

Genome-wide association study reveals growth-related mutations and candidate genes at early growth stages in largemouth bass (Micropterus salmoides)

Aquaculture, Journal Year: 2025, Volume and Issue: unknown, P. 742184 - 742184

Published: Jan. 1, 2025

Language: Английский