Genotoxicity Assessment of Co(II) and Ni(II) in HepG2 Cells: Insights into Combined Metal Exposure DOI
Alicia Thiel,

Sarah Heider,

Kira Bieck

et al.

Chemical Research in Toxicology, Journal Year: 2025, Volume and Issue: unknown

Published: April 8, 2025

The usage of cobalt (Co) and nickel (Ni) in numerous commercial, industrial, military applications causes widespread exposure nowadays, concerns are rising about adverse impacts on human health. Emphasis is the respiratory system, with both metals classified as (possibly) carcinogenic upon inhalation by International Agency for Research Cancer (IARC), but limited data available oral exposure. Therefore, this study aims to evaluate vitro genotoxicity Co(II) Ni(II) their combination HepG2 cells, since those environmental pollutants occurs realistically concert. Here, led induction single-strand breaks oxidative DNA damage detected Comet assay FPG-sensitive sites, while increased abundance γ-H2AX, an indicator double-strand breaks. Notably, combined resulted enhanced damage, especially at chromosomal level, formation micronuclei well polynucleated indicating a stronger effect compared single Furthermore, induced response pathway PARylation. As process involves consumption large amounts cellular NAD+ after energy state was assessed Ni(II). Current indicate that altered state. This reveals distinct mechanisms exhibited Ni(II), which were treatment. highlights need further research estimate genotoxic potential targeting cells intake increasing entry.

Language: Английский

Differential interactions of essential and toxic metal ions with biologically relevant phosphatidic acid and phosphatidylserine membranes DOI
Travis Issler, Kevin Sule,

Anna-Marie Lewrenz

et al.

BioMetals, Journal Year: 2024, Volume and Issue: 37(3), P. 631 - 648

Published: Jan. 30, 2024

Language: Английский

Citations

4
Single is not combined: The role of Co and Ni bioavailability on toxicity mechanisms in liver and brain cells DOI Creative Commons
Alicia Thiel,

Vivien Michaelis,

Marco Restle

et al.

Chemosphere, Journal Year: 2024, Volume and Issue: 357, P. 142091 - 142091

Published: April 20, 2024

The two trace elements cobalt (Co) and nickel (Ni) are widely distributed in the environment due to increasing industrial application, for example lithium-ion batteries. Both metals known cause detrimental health impacts humans when overexposed both supposed be a risk factor various diseases. individual toxicity of Co Ni has been partially investigated, however underlying mechanisms, as well interactions remain unknown. In this study, we focused on treatment liver carcinoma (HepG2) astrocytoma (CCF-STTG1) cells model target sites these metals. We investigated their effects single combined exposure cell survival, death bioavailability, induction oxidative stress. combination CoCl2 NiCl2 resulted higher levels with subsequent decreased amount compared treatment. Only led RONS increased GSSG formation, while apoptosis necrosis seem involved mechanisms NiCl2. Collectively, study demonstrates cell-type specific toxicity, HepG2 representing more sensitive line. Importantly, is toxic than exposure, which may originate partly from respective cellular content. Our data imply that major mechanism joint associated More studies needed assess after such advance an improved hazard prediction less artificial real-life scenarios.

Language: Английский

Citations

4
Cadmium-cardiolipin disruption of respirasome assembly and redox balance through mitochondrial membrane rigidification DOI Creative Commons
Nadiya Romanova, Kevin Sule, Travis Issler

et al.

Journal of Lipid Research, Journal Year: 2025, Volume and Issue: unknown, P. 100750 - 100750

Published: Jan. 1, 2025

Language: Английский

Citations

0
Metals associated with Beach plastic litter at Woodbridge Island and Derdesteen in Table Bay, South Africa DOI Creative Commons
Adetunji Awe,

Ogheneochuko Oputu,

Uyiosa Osagie Aigbe

et al.

Microplastics and Nanoplastics, Journal Year: 2025, Volume and Issue: 5(1)

Published: April 6, 2025

Language: Английский

Citations

0
Genotoxicity Assessment of Co(II) and Ni(II) in HepG2 Cells: Insights into Combined Metal Exposure DOI
Alicia Thiel,

Sarah Heider,

Kira Bieck

et al.

Chemical Research in Toxicology, Journal Year: 2025, Volume and Issue: unknown

Published: April 8, 2025

The usage of cobalt (Co) and nickel (Ni) in numerous commercial, industrial, military applications causes widespread exposure nowadays, concerns are rising about adverse impacts on human health. Emphasis is the respiratory system, with both metals classified as (possibly) carcinogenic upon inhalation by International Agency for Research Cancer (IARC), but limited data available oral exposure. Therefore, this study aims to evaluate vitro genotoxicity Co(II) Ni(II) their combination HepG2 cells, since those environmental pollutants occurs realistically concert. Here, led induction single-strand breaks oxidative DNA damage detected Comet assay FPG-sensitive sites, while increased abundance γ-H2AX, an indicator double-strand breaks. Notably, combined resulted enhanced damage, especially at chromosomal level, formation micronuclei well polynucleated indicating a stronger effect compared single Furthermore, induced response pathway PARylation. As process involves consumption large amounts cellular NAD+ after energy state was assessed Ni(II). Current indicate that altered state. This reveals distinct mechanisms exhibited Ni(II), which were treatment. highlights need further research estimate genotoxic potential targeting cells intake increasing entry.

Language: Английский

Citations

0