Exploring the Theoretical Propensity for Liquid-Liquid Phase Separation in the Organization of Golgi Matrix Proteins DOI Creative Commons
Luís F.S. Mendes, Carolina Boschi Gimenes, Emanuel Kava

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: July 22, 2023

Abstract The Golgi apparatus is a critical organelle in protein sorting and lipid metabolism. Characterized by its stacked, flattened cisternal structure, the exhibits distinct polarity with cis - trans -faces orchestrating various maturation transport processes. At heart of structural integrity organization are Matrix Proteins (GMPs), predominantly comprising Golgins GRASPs. These proteins contribute to this organelle’s unique stacked polarized structure ensure precise localization Golgi-resident enzymes, which crucial for accurate processing. Despite over century research since discovery, architecture’s intricate mechanisms still need be fully understood. Here, we demonstrate that GMPs present significant tendency form biocondensates through Liquid-Liquid Phase Separation (LLPS) across different Eukaryotic lineages. Moreover, validated experimentally members GRASP family also exhibit strong LLPS. Our findings offer new perspective on possible roles disorder LLPS organization.

Language: Английский

Membrane protein clustering from tension and multibody interactions DOI
Jean‐Baptiste Fournier

EPL (Europhysics Letters), Journal Year: 2024, Volume and Issue: 146(5), P. 57001 - 57001

Published: May 13, 2024

The point-curvature model for membrane protein inclusions is shown to capture multibody interactions very well. Using this model, we find that the interplay between tension and results in a collective attraction of oppositely curved tending form antiferromagnetic structures with square lattice. This can produce phase separation non-curved proteins, resulting clustering proteins. We also show many-body repulsion identical proteins enhanced by tension. lead dissolution clusters stabilized short-range forces when increased. These new phenomena are biologically relevant could be investigated experimentally

Language: Английский

Citations

2
Exploring liquid-liquid phase separation in the organisation of Golgi matrix proteins DOI
Luís F.S. Mendes, Carolina Boschi Gimenes,

Kevin F. Simões

et al.

Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, Journal Year: 2024, Volume and Issue: 1872(5), P. 141029 - 141029

Published: June 24, 2024

Language: Английский

Citations

1
Quantification of biomolecular condensate volume reveals network swelling and dissolution regimes during phase transition DOI Creative Commons
Iris B. A. Smokers, Evan Spruijt

Published: Aug. 14, 2024

Accurate determination of biomolecular condensate volume reveals that destabilization condensates can lead to either swelling or shrinking condensates, giving fundamental insights into regulation the cellular condensates. Determination and coacervate protocells is essential investigate their precise composition impact on (bio)chemical reactions are localized inside It not a straightforward task, as have tiny volumes, highly viscous prone wetting. Here we examine different strategies determine volume, introduce two new methods, with which volumes 1 μL less (volume fraction 0.4%) be determined standard deviation 0.03 μL. Using these methods show depends degree physical crosslinking. These observations supported by Flory-Huggins theory profound effects in cell biology.

Language: Английский

Citations

1
Special issue: 50 years of the fluid mosaic model for cell membranes DOI Creative Commons
Burkhard Bechinger

Biochimica et Biophysica Acta (BBA) - Biomembranes, Journal Year: 2023, Volume and Issue: 1865(7), P. 184181 - 184181

Published: May 31, 2023

Language: Английский

Citations

1
Adhesion energy controls lipid binding-mediated endocytosis DOI Open Access
Raluca Groza,

Kita Valerie Schmidt,

Paul Markus Müller

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: June 25, 2023

Abstract Several bacterial toxins and viruses can deform membranes through multivalent binding to lipids for clathrin-independent endocytosis. However, it remains unclear, how membrane deformation endocytic internalization are mechanistically linked. Here we show that many lipid-binding virions induce endocytosis, suggesting a common mechanism based on lipid by globular particles. We create synthetic cellular system consisting of lipid-anchored receptor in the form GPI-anchored anti-GFP nanobodies binder exposing 180 regularly-spaced GFP molecules its surface. these globular, 40 nm diameter, particles bind cells expressing receptor, plasma upon adhesion become endocytosed manner. explore role energy endocytosis using receptors with affinities varying over 7 orders magnitude. Using this system, find once threshold is overcome allow deformation, occurs reliably. Multivalent, binding-induced binders thus sufficient occur suggest common, purely biophysical mediated pathogens.

Language: Английский

Citations

1
Protein droplets spread to seal tight junctions DOI
Alexander Ludwig

Nature, Journal Year: 2024, Volume and Issue: 632(8025), P. 506 - 507

Published: Aug. 7, 2024

Language: Английский

Citations

0
Scanning probe microscopy elucidates gelation and rejuvenation of biomolecular condensates DOI Creative Commons
Aida Naghilou, Oskar Armbruster, Alireza Mashaghi

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 29, 2024

Abstract Comprehensive understanding of dynamics and disease-associated solidification biomolecular condensates is closely tied to analysis their mechanical characteristics. Despite recent technical advances in rheological studies condensates, these still vastly rely on methods restricted small forces, rendering measurements droplets with higher elasticities after transition solid challenging. Here, we develop assays for in-depth characterization by scanning probe microscopy. We demonstrate this technique measuring the behavior heterotypic poly-L-lysine heparin showcasing multi-route liquid gel transition, as well rejuvenation chemical alterations medium. Due wide-spread application microscopy biological fields, its capability rapid, high throughput, force range studies, integration nanoscale morphological measurements, our probe-based method a significant breakthrough investigating condensate behavior, leading accelerated development therapies.

Language: Английский

Citations

0
Quantification of Biomolecular Condensate Volume Reveals Network Swelling and Dissolution Regimes during Phase Transition DOI Creative Commons
Iris B. A. Smokers, Evan Spruijt

Biomacromolecules, Journal Year: 2024, Volume and Issue: 26(1), P. 363 - 373

Published: Dec. 2, 2024

Accurate determination of biomolecular condensate volume reveals that destabilization condensates can lead to either swelling or shrinking condensates, giving fundamental insights into the regulation cellular condensates. Determination and coacervate protocells is essential investigate their precise composition impact on (bio)chemical reactions are localized inside It not a straightforward task, as have tiny volumes, highly viscous, prone wetting. Here, we examine different strategies determine introduce two new methods, with which volumes 1 μL less (volume fraction 0.4%) be determined standard deviation 0.03 μL. Using these show depends degree physical cross-linking. These observations supported by Flory–Huggins theory profound effects in cell biology.

Language: Английский

Citations

0
Exploring the Theoretical Propensity for Liquid-Liquid Phase Separation in the Organization of Golgi Matrix Proteins DOI Creative Commons
Luís F.S. Mendes, Carolina Boschi Gimenes, Emanuel Kava

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: July 22, 2023

Abstract The Golgi apparatus is a critical organelle in protein sorting and lipid metabolism. Characterized by its stacked, flattened cisternal structure, the exhibits distinct polarity with cis - trans -faces orchestrating various maturation transport processes. At heart of structural integrity organization are Matrix Proteins (GMPs), predominantly comprising Golgins GRASPs. These proteins contribute to this organelle’s unique stacked polarized structure ensure precise localization Golgi-resident enzymes, which crucial for accurate processing. Despite over century research since discovery, architecture’s intricate mechanisms still need be fully understood. Here, we demonstrate that GMPs present significant tendency form biocondensates through Liquid-Liquid Phase Separation (LLPS) across different Eukaryotic lineages. Moreover, validated experimentally members GRASP family also exhibit strong LLPS. Our findings offer new perspective on possible roles disorder LLPS organization.

Language: Английский

Citations

0