Journal of Drug Delivery Science and Technology, Journal Year: 2024, Volume and Issue: 92, P. 105349 - 105349
Published: Jan. 6, 2024
Language: Английский
Molecular Cancer, Journal Year: 2023, Volume and Issue: 22(1)
Published: Oct. 18, 2023
Abstract Cancer stem-like cells (CSCs), a subpopulation of cancer cells, possess remarkable capability in proliferation, self-renewal, and differentiation. Their presence is recognized as crucial factor contributing to tumor progression metastasis. CSCs have garnered significant attention therapeutic focus an etiologic root treatment-resistant cells. Increasing evidence indicated that specific biomarkers, aberrant activated pathways, immunosuppressive microenvironment (TME), immunoevasion are considered the culprits occurrence maintenance properties including multi-directional Targeting CSC stemness-associated TME, inducing differentiation improve eradication and, therefore, treatment. This review comprehensively summarized these targeted therapies, along with their current status clinical trials. By exploring implementing strategies aimed at eradicating CSCs, researchers aim treatment outcomes overcome challenges posed by CSC-mediated therapy resistance.
Language: Английский
Citations
43
Nature reviews. Cancer, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 28, 2025
Language: Английский
Citations
3
Journal of Experimental & Clinical Cancer Research, Journal Year: 2025, Volume and Issue: 44(1)
Published: Jan. 14, 2025
Abstract Background Most patients with prostate cancer inevitably progress to castration-resistant (CRPC), at which stage chemotherapeutics like docetaxel become the first-line treatment. However, chemotherapy resistance typically develops after an initial period of therapeutic efficacy. Increasing evidence indicates that stem cells confer via exosomes. This study demonstrated AGD1, derived from (PCSCs), enhanced stemness and reduced effect in CRPC. Methods Quantitative real-time PCR (qPCR) was employed determine expression levels AGD1 METTL13 mRNAs PCSCs Protein were examined using western blots dot blots. The potential functions CRPC investigated through cell proliferation assay, Transwell EdU incorporation assays, Annexin V-FITC/PI staining, sphere formation assays. To uncover underlying mechanisms RNA pull-down RIP, co-Immunoprecipitation (co-IP), mass spectrometry (MS), Methylated immunoprecipitation (MeRIP) single-base elongation ligation-based qPCR amplification method (SELECT) performed. effects on development metastasis under treatment analyzed a xenograft mouse model organoid model. Additionally, liposomal-chitosan nanocomplex drug delivery systems designed explore AGD1’s role regulating Results upregulated Downregulating sensitivity by inhibiting their stemness, reverse also being true. pull-down, combined MS, co-IP RIP binds USP10, forming complex facilitates protein accumulation USP10-induced deubiquitination. MeRIP assay SELECT revealed transcriptionally controls mRNA decay CD44 m6A methylation. this process activates pSTAT3/PI3K-AKT signaling pathway. Organoid models showed reducing Conclusions mediates apoptosis promotes enhancing tumor growth USP10/METTL13-mediated
Language: Английский
Citations
2
Research, Journal Year: 2023, Volume and Issue: 6
Published: Jan. 1, 2023
Prostate cancer (PCa) is a common malignant tumor with high morbidity and mortality worldwide. The prostate stem cell (PCSC) model provides novel insights into the pathogenesis of PCa its therapeutic response. However, roles molecular mechanisms specific genes in mediating fate decisions PCSCs carcinogenesis remain to be elusive. In this study, we have explored expression, function, mechanism AZGP1P2, pseudogene AZGP1, regulating stemness apoptosis treatment resistance docetaxel castration-resistant (CRPC). We revealed that AZGP1P2 was downregulated CRPC lines PCSCs, while it positively associated progression-free interval. Upregulation enhanced sensitivity CRPCs via inhibiting their stemness. RNA pull-down mass spectrometry analysis, co-immunoprecipitation assay, immunoprecipitation assay demonstrated could bind UBA1 RBM15 as "writer" methyltransferase form compound. UBA1, an E1 ubiquitin-activating enzyme, contributed protein degradation ubiquitination modification. Methylated displayed controlled mRNA decay TPM1 m6A methylation. Furthermore, xenograft mouse patient-derived organoids showed effect increased by vivo. Collectively, these results imply mediates promotes suppressing growth metastasis UBA1/RBM15-mediated CRPC.
Language: Английский
Citations
33
Oncogene, Journal Year: 2023, Volume and Issue: 42(46), P. 3371 - 3384
Published: Oct. 17, 2023
Abstract Epithelial tissue homeostasis is closely associated with the self-renewal and differentiation behaviors of epithelial stem cells (ESCs). p63, a well-known marker ESCs, an indispensable factor for their biological activities during development. The diversity p63 isoforms expressed in distinct tissues allows this transcription to have wide array effects. coordinates genes involved cell survival, self-renewal, migration, differentiation, epithelial-to-mesenchymal transition. Through regulation these processes, contributes to, not only normal development, but also epithelium-derived cancer pathogenesis. In review, we provide overview role stemness regulation, including proliferation, senescence. We describe differential expression TAp63 ΔNp63 functional tumors. Furthermore, summarize signaling cascades modulating as well downstream pathways regulation.
Language: Английский
Citations
32
Current Tissue Microenvironment Reports, Journal Year: 2024, Volume and Issue: 5(2), P. 39 - 59
Published: Jan. 25, 2024
Language: Английский
Citations
13
Oncology Letters, Journal Year: 2025, Volume and Issue: 29(3)
Published: Jan. 14, 2025
Cancer stem cells (CSCs) contribute to the resistance of intractable prostate cancer, and dopamine receptor (DR)D2 antagonists exhibit anticancer activity against cancer CSCs. Human PC-3 were used generate CSC-like cells, serving as a surrogate system identify specific DR subtype inhibition which significantly affects prostate-derived Additionally, present study aimed determine downstream signaling molecules this that exert more profound effects compared with other subtypes. The inhibitory or small interfering (si)RNAs on subtypes by analyzing morphological changes expression patterns pluripotency markers, cell growth activities in vitro invasion. L-741,626, DRD2 antagonist, induced PC-3-derived suppressed Oct4 (a marker), inhibited tumors. proliferation heterozygous null generated using CRISPR/Cas9 method, was slow, their sphere-forming ability substantially reduced, indicating diminished capacity produce In addition, phosphorylation AMPK siRNA knockout may be putative molecule involved production maintenance cells. Specific suppression caused lose properties formation followed AMPK, is considered DRD2. Further understanding mechanisms regulates provide valuable insights into identification molecular targets for treating wherein constitutively activated.
Language: Английский
Citations
1
Breast Cancer Research, Journal Year: 2025, Volume and Issue: 27(1)
Published: Jan. 28, 2025
Language: Английский
Citations
1
MedComm – Oncology, Journal Year: 2025, Volume and Issue: 4(1)
Published: Feb. 17, 2025
ABSTRACT Metastasis remains a leading cause of cancer‐related deaths, defined by complex, multi‐step process in which tumor cells spread and form secondary growths distant tissues. Despite substantial progress understanding metastasis, the molecular mechanisms driving this development effective therapies remain incompletely understood. Elucidating pathways governing metastasis is essential for discovery innovative therapeutic targets. The rapid advancements sequencing technologies expansion biological databases have significantly deepened our drivers associated drug resistance. This review focuses on particularly roles genetic mutations, epigenetic changes, post‐translational modifications progression. We also examine how microenvironment influences metastatic behavior explore emerging strategies, including targeted immunotherapies. Finally, we discuss future research directions, stressing importance novel treatment approaches personalized strategies to overcome improve patient outcomes. By integrating contemporary insights into basis innovation, provides comprehensive framework guide clinical cancer.
Language: Английский
Citations
1
Discover Oncology, Journal Year: 2025, Volume and Issue: 16(1)
Published: March 22, 2025
Cancer stem cells (CSCs), are a critical subpopulation within tumours, and defined by their capacity for self-renewal, differentiation, tumour initiation. These unique traits contribute to progression, metastasis, resistance conventional treatments like chemotherapy radiotherapy, often resulting in cancer recurrence poor patient outcomes. As such, CSCs have become focal points developing advanced therapies. This review highlights progress CSC-targeted treatments, including chimeric antigen receptor T-cell (CAR-T) therapy, immunotherapy, molecular targeting, nanoparticle-based drug delivery systems. Plant-derived compounds gene-editing technologies, such as clustered regularly interspaced short palindromic repeats (CRISPR), explored potential enhance precision minimize side effects. Metabolic pathways integral CSC survival, mitochondrial dynamics, mitophagy (regulated dynamin-related protein 1 [DRP1] the PINK1/Parkin pathway), one-carbon metabolism, amino acid metabolism (involving enzymes glutaminase (GLS) glutamate dehydrogenase (GDH]), lipid hypoxia-induced metabolic reprogramming mediated hypoxia-inducible factors (HIF-1α HIF-2α), examined therapeutic targets. The adaptability of through autophagy, flexibility, epigenetic regulation metabolites α-ketoglutarate, succinate, fumarate is discussed. Additionally, extracellular vesicles nicotinamide adenine dinucleotide (NAD⁺) identified pivotal redox balance, DNA repair, modifications. Addressing challenges heterogeneity, immune evasion, treatment durability requires interdisciplinary collaboration. Advancing therapies essential overcoming preventing relapse, paving way transformative treatments. underscores importance leveraging innovative technologies fostering collaboration revolutionize treatment.
Language: Английский
Citations
1