Ivermectin Synergizes with Modulated Electro-hyperthermia and Improves Its Anticancer Effects in a Triple-Negative Breast Cancer Mouse Model DOI Creative Commons
Kenan Aloss, Pedro Henrique Leroy Viana, Syeda Mahak Zahra Bokhari

et al.

ACS Pharmacology & Translational Science, Journal Year: 2024, Volume and Issue: 7(8), P. 2496 - 2506

Published: July 17, 2024

Triple-negative breast cancer (TNBC) is the most aggressive subtype, with limited treatment options. Modulated electro-hyperthermia (mEHT) a novel adjuvant therapy that induces selective damage. However, mEHT upregulates heat shock protein beta 1 (HSPB1), cancer-promoting stress chaperone molecule. Thus, we investigated whether ivermectin (IVM), an anthelmintic drug, may synergize and enhance its anticancer effects by inhibiting HSPB1 phosphorylation. Isogenic 4T1 TNBC cells were inoculated into BALB/c mice treated mEHT, IVM, or combination of both. IVM synergistically improved tumor growth inhibition achieved mEHT. Moreover, downregulated mEHT-induced strongest tissue damage was observed in + IVM-treated tumors, coupled apoptosis induction proliferation inhibition. In addition, there no significant body weight loss indicating this well-tolerated. conclusion, combined new, effective, safe option for TNBC.

Language: Английский

The Interplay between Heat Shock Proteins and Cancer Pathogenesis: A Novel Strategy for Cancer Therapeutics DOI Open Access
Prathap Somu, Sonali Mohanty, Nagaraj Basavegowda

et al.

Cancers, Journal Year: 2024, Volume and Issue: 16(3), P. 638 - 638

Published: Feb. 1, 2024

Heat shock proteins (HSPs) are developmentally conserved families of protein found in both prokaryotic and eukaryotic organisms. HSPs engaged a diverse range physiological processes, including molecular chaperone activity to assist the initial folding or promote unfolding refolding misfolded intermediates acquire normal native conformation its translocation prevent aggregation as well immunity, apoptosis, autophagy. These chaperonins classified into various according their size weight, encompassing small (e.g., HSP10 HSP27), HSP40, HSP60, HSP70, HSP90, category large that include HSP100 ClpB proteins. The overexpression is induced counteract cell stress at elevated levels variety solid tumors, anticancer chemotherapy, closely related worse prognosis therapeutic resistance cancer cells. also involved anti-apoptotic properties associated with processes progression development, such metastasis, invasion, proliferation. This review outlines previously mentioned significant involvement mechanisms tumor advancement contribution identifying potential targets for interventions.

Language: Английский

Citations

16
Synergistic effects of 6-shogaol and hyperthermia on ACHN renal cancer cells: modulation of ROS and heat shock pro-teins in cancer therapy DOI Creative Commons
Chae Ryeong Ahn, Seung Ho Baek

Frontiers in Pharmacology, Journal Year: 2025, Volume and Issue: 16

Published: Feb. 20, 2025

Renal cancer is known for its aggressive progression and resistance to standard treatments, underscoring the need novel therapeutic strategies. This study explores potential of combining 6-shogaol (6-SHO), a bioactive compound derived from ginger (Zingiber officinale), with hyperthermia enhance anticancer efficacy in ACHN renal cells. cells were treated 6-SHO exposed hyperthermic conditions. We evaluated combined effects on apoptosis, cell cycle arrest, proliferation, as well role reactive oxygen species (ROS) heat shock proteins (HSPs) mediating these responses. The combination significantly increased induced G2/M phase reduced proliferation more effectively than either treatment alone. ROS played critical effects, modulation HSPs factor 1 (HSF1) further disrupting survival mechanisms. These findings highlight synergistic approach treatment, supporting research clinical evaluation.

Language: Английский

Citations

0
Chemoradiotherapy Combined With Modulated Electro‐Hyperthermia (mEHT) Converts Initially Inoperable Esophageal Cancer to Operable Disease DOI Creative Commons
Deng‐Yu Kuo, Pei‐Wei Shueng,

Shan‐Ying Wang

et al.

Thoracic Cancer, Journal Year: 2025, Volume and Issue: 16(6)

Published: March 1, 2025

ABSTRACT This case report demonstrated the effectiveness of combining chemoradiotherapy (CCRT) with modulated electro‐hyperthermia (mEHT) in converting initially inoperable esophageal cancer into an operable condition. A 60‐year‐old male stage IVB lower third squamous cell carcinoma (cT3N2M1) experienced significant tumor shrinkage (ypT2N0M0) and a notable metabolic response, which enabled successful surgical removal. treatment approach highlights potential mEHT as valuable addition to comprehensive management cancer.

Language: Английский

Citations

0
Heat shock factor 1 inhibition enhances the effects of modulated electro hyperthermia in a triple negative breast cancer mouse model DOI Creative Commons
Pedro Henrique Leroy Viana,

Csaba András Schvarcz,

Lea Danics

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: April 8, 2024

Female breast cancer is the most diagnosed worldwide. Triple negative (TNBC) aggressive type and there no existing endocrine or targeted therapy. Modulated electro-hyperthermia (mEHT) a non-invasive complementary therapy using an electromagnetic field generated by amplitude modulated 13.56 MHz frequency that induces tumor cell destruction. However, we have demonstrated strong induction of heat shock response (HSR) mEHT, which can result in thermotolerance. We hypothesized inhibition factor 1 (HSF1) synergize with mEHT enhance cell-killing. Thus, either knocked down HSF1 gene CRISPR/Cas9 lentiviral construct inhibited specific small molecule inhibitor: KRIBB11 vivo. Wild HSF1-knockdown 4T1 TNBC cells were inoculated into mammary gland's fat pad BALB/c mice. Four treatments performed every second day growth was followed ultrasound caliper. administrated intraperitoneally at 50 mg/kg daily for 8 days. Hsp70 expression assessed. knockdown sensitized transduced to reduced growth. mRNA significantly KO group when compared empty vector group, consequently mEHT-induced upregulation diminished group. Immunohistochemistry (IHC) confirmed HSF1-KO Demonstrating translational potential inhibition, combined mass monotherapy. Inhibition also supported qPCR IHC. In conclusion, suggest mEHT-therapy be possible new strategy treatment great potential.

Language: Английский

Citations

3
Regulation of transcription factor function by purinergic signalling in cardiovascular diseases DOI
Hao Tang,

Qihang Kong,

Zhewei Zhang

et al.

Purinergic Signalling, Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 31, 2024

Language: Английский

Citations

0
Case report: Combinations of immune checkpoint inhibitor, chemotherapy, and hyperthermia therapy avoid lymphatic recurrence in cholangiocarcinoma DOI Creative Commons
Heng-Jui Chang, Chiao‐Hsu Ke, Yu‐Shan Wang

et al.

Frontiers in Oncology, Journal Year: 2024, Volume and Issue: 14

Published: Oct. 24, 2024

Cholangiocarcinoma is a malignancy known for its aggressiveness and limited treatment options. The malignant tumor behaviors include intrahepatic recurrence, regional lymph node (LN) metastasis, peritoneal carcinomatosis, lung metastasis. Herein, we reported case of lymphatic recurrence in an cholangiocarcinoma patient after surgery, adjuvant concurrent chemoradiotherapy (CCRT), who experienced remarkable response to combination therapy. However, the failed undergo radiotherapy or other invasive local therapy therefore received Opdivo (nivolumab) with chemotherapy (FOLFOX) modulated electro-hyperthermia. Notably, these medical interventions, this had complete (CR) treatments, which no metastasis occurred, significantly decreased marker, CA 19-9, level was found. This highlights potential multiple anti-tumor therapies, including immune checkpoint inhibitors, chemotherapy, hyperthermia, managing challenging cases.

Language: Английский

Citations

0
Ivermectin Synergizes with Modulated Electro-hyperthermia and Improves Its Anticancer Effects in a Triple-Negative Breast Cancer Mouse Model DOI Creative Commons
Kenan Aloss, Pedro Henrique Leroy Viana, Syeda Mahak Zahra Bokhari

et al.

ACS Pharmacology & Translational Science, Journal Year: 2024, Volume and Issue: 7(8), P. 2496 - 2506

Published: July 17, 2024

Triple-negative breast cancer (TNBC) is the most aggressive subtype, with limited treatment options. Modulated electro-hyperthermia (mEHT) a novel adjuvant therapy that induces selective damage. However, mEHT upregulates heat shock protein beta 1 (HSPB1), cancer-promoting stress chaperone molecule. Thus, we investigated whether ivermectin (IVM), an anthelmintic drug, may synergize and enhance its anticancer effects by inhibiting HSPB1 phosphorylation. Isogenic 4T1 TNBC cells were inoculated into BALB/c mice treated mEHT, IVM, or combination of both. IVM synergistically improved tumor growth inhibition achieved mEHT. Moreover, downregulated mEHT-induced strongest tissue damage was observed in + IVM-treated tumors, coupled apoptosis induction proliferation inhibition. In addition, there no significant body weight loss indicating this well-tolerated. conclusion, combined new, effective, safe option for TNBC.

Language: Английский

Citations

0