Exploration of the multiomics-based mechanisms of Gancao Nourishing-Yin decoction in regulating mitochondrial metabolic genes CYB5R3 and PICK1 to influence glioma progression DOI Creative Commons
Yufeng Chen, Anjing Zhang, Lei Li

et al.

Discover Oncology, Journal Year: 2025, Volume and Issue: 16(1)

Published: April 8, 2025

Glioma is the most common malignancy of central nervous system, characterised by its high invasiveness and recurrence, which significantly limit therapeutic outcomes. Energy metabolism reprogramming, especially mitochondrial dysfunction, plays a pivotal role in tumour growth, survival, progression. Mitochondria serve as hub for energy production biosynthesis, adapting through alterations oxidative phosphorylation, lipid metabolism, tricarboxylic acid cycle to meet metabolic demands gliomas. Gancao Nourishing-Yin (GCNY) decoction, traditional herbal compound, has demonstrated potential antitumour effects, particularly modulating pathways stress, are critical cell adaptation. Genomic data from glioma GWAS dataset Finnish R11 database eQTL 13 distinct brain regions were analysed using Summary-data-based Mendelian Randomization(SMR) identify glioma-associated genes. MitoCarta3.0, genes, was used pinpoint mitochondrial-related Differentially expressed genes (DEGs) human microglial cells treated with GCNY extracted GEO GSE210945 cross-referenced SMR results key Colocalisation analysis, validation TCGA database, survival functional annotations performed explore mechanisms action. Integration MitoCarta3.0 identified 19 linked gliomas, primarily involved amino fatty metabolism. Among these, CYB5R3 PICK1 emerged strong genetic links signals (PPH4 = 1). CYB5R3, upregulated associated enhanced elevated reactive oxygen species (ROS) production, poor outcomes (HR 2.23, P < 0.001). PICK1, despite being downregulated, showed context-dependent roles progression, expression worse prognosis 2.86, moderate predictive capacity one-year (AUC 0.695). This study highlights identifying regulators influenced GCNY. By pathways, including ROS phosphorylation activity, offers novel multitarget strategy therapy. These findings underscore importance target gliomas provide new insights into integrated management.

Language: Английский

Identification of telomere-related gene subtypes and prognostic signatures in osteosarcoma DOI Creative Commons
Z. Song, Wenyan Yu, Xuqing Yin

et al.

Frontiers in Pharmacology, Journal Year: 2025, Volume and Issue: 16

Published: Feb. 25, 2025

Osteosarcoma (OS) is the prevalent primary bone cancer, with a high proclivity for local invasion and metastasis. Previous studies have indicated that telomeres are closely related to prognosis of but significance telomere-related features in OS remains uncertain. Thus, goal this work identified subtypes based on genes (TRGs). The data was collected from TARGET Gene Expression Omnibus databases. Firstly, we mediated by TRGs OS. Subsequently, analyzed immune characteristics telomeres-related Moreover, built signature via univariate LASSO Cox regression analyses, correlation TME. Finally, expression hub We discovered could distinguish patients into two (C1 C2). survival rate C2 inferior C1. scores stromal, ESTIMATES were observably increased, tumor purity decreased C1 compared subtypes. Differentially expressed between highly enriched immune-related pathways. had different characteristic. Furthermore, telomere prognostic model including six (PDK2, PPARG, MORC4, SP110, TERT MAP3K5) established predict patients. High-risk group correlated patients, risk score PDK2, MAP3K5 significantly cells. In conclusion, our study has uncovered importance defining distinct outcomes contexts. developed may serve as valuable tool prediction inform future therapeutic strategies targeting TME

Language: Английский

Citations

0
Exploration of the multiomics-based mechanisms of Gancao Nourishing-Yin decoction in regulating mitochondrial metabolic genes CYB5R3 and PICK1 to influence glioma progression DOI Creative Commons
Yufeng Chen, Anjing Zhang, Lei Li

et al.

Discover Oncology, Journal Year: 2025, Volume and Issue: 16(1)

Published: April 8, 2025

Glioma is the most common malignancy of central nervous system, characterised by its high invasiveness and recurrence, which significantly limit therapeutic outcomes. Energy metabolism reprogramming, especially mitochondrial dysfunction, plays a pivotal role in tumour growth, survival, progression. Mitochondria serve as hub for energy production biosynthesis, adapting through alterations oxidative phosphorylation, lipid metabolism, tricarboxylic acid cycle to meet metabolic demands gliomas. Gancao Nourishing-Yin (GCNY) decoction, traditional herbal compound, has demonstrated potential antitumour effects, particularly modulating pathways stress, are critical cell adaptation. Genomic data from glioma GWAS dataset Finnish R11 database eQTL 13 distinct brain regions were analysed using Summary-data-based Mendelian Randomization(SMR) identify glioma-associated genes. MitoCarta3.0, genes, was used pinpoint mitochondrial-related Differentially expressed genes (DEGs) human microglial cells treated with GCNY extracted GEO GSE210945 cross-referenced SMR results key Colocalisation analysis, validation TCGA database, survival functional annotations performed explore mechanisms action. Integration MitoCarta3.0 identified 19 linked gliomas, primarily involved amino fatty metabolism. Among these, CYB5R3 PICK1 emerged strong genetic links signals (PPH4 = 1). CYB5R3, upregulated associated enhanced elevated reactive oxygen species (ROS) production, poor outcomes (HR 2.23, P < 0.001). PICK1, despite being downregulated, showed context-dependent roles progression, expression worse prognosis 2.86, moderate predictive capacity one-year (AUC 0.695). This study highlights identifying regulators influenced GCNY. By pathways, including ROS phosphorylation activity, offers novel multitarget strategy therapy. These findings underscore importance target gliomas provide new insights into integrated management.

Language: Английский

Citations

0