Microfluidics-free single-cell genomics reveals complex central-peripheral immune crosstalk in the mouse brain during peripheral inflammation DOI Creative Commons
Malú G. Tansey, Jake Boles, Oihane Uriarte Huarte

et al.

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: Oct. 17, 2023

Inflammation is a realized detriment to brain health in growing number of neurological diseases, but querying neuroinflammation its cellular complexity remains challenge. This manuscript aims provide reliable and accessible strategy for examining the brain's immune system. We compare efficacy cell isolation methods producing ample pure samples from mouse brains. Then, with high-input single-cell genomics platform PIPseq, we generate rich neuroimmune dataset containing microglia many peripheral populations. To demonstrate this strategy's utility, interrogate well-established model LPS-induced resolution. activation crosstalk between phagocytes highlight unique contributions cells neuroinflammation. Our approach enables high-depth evaluation inflammation longstanding rodent models disease reveal novel insight into system health.

Language: Английский

Microfluidics-free single-cell genomics reveals complex central-peripheral immune crosstalk in the mouse brain during peripheral inflammation DOI Creative Commons
Malú G. Tansey, Jake Boles, Oihane Uriarte Huarte

et al.

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: Oct. 17, 2023

Inflammation is a realized detriment to brain health in growing number of neurological diseases, but querying neuroinflammation its cellular complexity remains challenge. This manuscript aims provide reliable and accessible strategy for examining the brain's immune system. We compare efficacy cell isolation methods producing ample pure samples from mouse brains. Then, with high-input single-cell genomics platform PIPseq, we generate rich neuroimmune dataset containing microglia many peripheral populations. To demonstrate this strategy's utility, interrogate well-established model LPS-induced resolution. activation crosstalk between phagocytes highlight unique contributions cells neuroinflammation. Our approach enables high-depth evaluation inflammation longstanding rodent models disease reveal novel insight into system health.

Language: Английский

Citations

1