Insulin-Like Growth Factor-1 Is Neuroprotective in Aged Rats With Ischemic Stroke DOI Creative Commons
Ahmad Serhan, Erik Boddeke, Ron Kooijman

et al.

Frontiers in Aging Neuroscience, Journal Year: 2019, Volume and Issue: 11

Published: Dec. 11, 2019

Post-stroke systemic injections of insulin-like growth factor 1 (IGF-1) exert neuroprotective effects in rats. In the current study, we aimed to test efficacy IGF-1 neuroprotection aged rats (24-25 months old) and compare results with adult (6-7 old). Furthermore, addressed putative differences microglial responses Rats were subjected ischemic stroke while they conscious by infusing endothelin-1 (Et-1) through a guide cannula that was implemented vicinity middle cerebral artery (MCA). given subcutaneous (1 mg/kg) at 30 min 120 after insult. treatment reduced infarct size 34% 38% rats, respectively. The treated also showed significant improvement sensorimotor function following stroke, this not significantly affected displayed exaggerated activation microglia hemisphere. Significant reduction only detected specific regions ipsilateral hemisphere We show an stroke. It remains be established, however, whether age-related changes affect behavioral outcome.

Language: Английский

Conditional deletion of LRRC8A in the brain reduces stroke damage independently of swelling-activated glutamate release DOI Creative Commons
Mustafa Balkaya, Preeti Dohare, Sophie Chen

et al.

iScience, Journal Year: 2023, Volume and Issue: 26(5), P. 106669 - 106669

Published: April 14, 2023

The ubiquitous volume-regulated anion channels (VRACs) facilitate cell volume control and contribute to many other physiological processes. Treatment with non-specific VRAC blockers or brain-specific deletion of the essential subunit LRRC8A is highly protective in rodent models stroke. Here, we tested widely accepted idea that harmful effects VRACs are mediated by release excitatory neurotransmitter glutamate. We produced conditional knockout either exclusively astrocytes majority brain cells. Genetically modified mice were subjected an experimental stroke (middle cerebral artery occlusion). astrocytic yielded no protection. Conversely, brain-wide strongly reduced infarction both heterozygous (Het) full KO mice. Yet, despite identical protection, Het had swelling-activated glutamate release, whereas animals showed its virtual absence. These findings suggest contributes ischemic injury via a mechanism than VRAC-mediated release.

Language: Английский

Citations

10
Behavioural assessment of neuropsychiatric outcomes in rodent stroke models DOI

R. Callaghan,

Huiyuan Yang, Rachel D. Moloney

et al.

Journal of Cerebral Blood Flow & Metabolism, Journal Year: 2025, Volume and Issue: unknown

Published: March 20, 2025

Stroke-associated mood disorders are less recognised than sensorimotor impairment, despite their high prevalence. Similarly, few experimental stroke studies assess non-sensorimotor functions. This study examined the prevalence and implementation of tests in three stroke-focused journals over last twenty years. Of 965 ischaemic papers which used behavioural testing rodents, 932 included testing, while 137 (most commonly Morris water maze, open field, Y-maze, novel object recognition tests, but with a more diverse range introduced recent years). Cognition, anxiety depression were assessed 70%, 27% 3% these papers. Non-sensorimotor deficits typically observed after recovery function. Potential confounding factors challenges for data interpretation identified most prevalent tests. More generally, rigor (a priori power calculation, randomisation, blinding, pre-defined inclusion/exclusion) improved years, remained unsatisfactory only 26% providing some evidence adequate statistical power. Furthermore, focused on male animals, limiting external validity. review confirms disparity between shows that share including latter is increasing. It essential research into neuropsychiatric sequalae addresses methodological issues noted continues to expand improve patient outcomes post-stroke.

Language: Английский

Citations

0
Future of Animal Modeling for Poststroke Tissue Repair DOI Open Access
Michel Modo, Jukka Jolkkonen, Marietta Zille

et al.

Stroke, Journal Year: 2018, Volume and Issue: 49(5), P. 1099 - 1106

Published: April 18, 2018

Language: Английский

Citations

29
Motor deficit in the mouse ferric chloride-induced distal middle cerebral artery occlusion model of stroke DOI

Nausheen Syeara,

Faisal F. Alamri,

Srinidhi Jayaraman

et al.

Behavioural Brain Research, Journal Year: 2019, Volume and Issue: 380, P. 112418 - 112418

Published: Dec. 5, 2019

Language: Английский

Citations

27
Insulin-Like Growth Factor-1 Is Neuroprotective in Aged Rats With Ischemic Stroke DOI Creative Commons
Ahmad Serhan, Erik Boddeke, Ron Kooijman

et al.

Frontiers in Aging Neuroscience, Journal Year: 2019, Volume and Issue: 11

Published: Dec. 11, 2019

Post-stroke systemic injections of insulin-like growth factor 1 (IGF-1) exert neuroprotective effects in rats. In the current study, we aimed to test efficacy IGF-1 neuroprotection aged rats (24-25 months old) and compare results with adult (6-7 old). Furthermore, addressed putative differences microglial responses Rats were subjected ischemic stroke while they conscious by infusing endothelin-1 (Et-1) through a guide cannula that was implemented vicinity middle cerebral artery (MCA). given subcutaneous (1 mg/kg) at 30 min 120 after insult. treatment reduced infarct size 34% 38% rats, respectively. The treated also showed significant improvement sensorimotor function following stroke, this not significantly affected displayed exaggerated activation microglia hemisphere. Significant reduction only detected specific regions ipsilateral hemisphere We show an stroke. It remains be established, however, whether age-related changes affect behavioral outcome.

Language: Английский

Citations

27