Development of a β-Arbutin Hyperproducer in Escherichia coli by Rational Modular and Transcription Factor Engineering DOI

Shuran Jiang,

Xuewei Pan, Xinyu Zhang

et al.

ACS Sustainable Chemistry & Engineering, Journal Year: 2024, Volume and Issue: 12(52), P. 18588 - 18599

Published: Dec. 18, 2024

β-Arbutin is a valuable aromatic compound with multiple biological activities. The exploitation of efficient engineering microbial platforms for β-arbutin production has been an appealing research avenue in recent years due to l-tyrosine auxotrophy and the limited distribution metabolic flux. Herein, inducer-free nonauxotrophic cell factory successfully constructed Escherichia coli toward high-titer by combining strategies. First, de novo synthesis pathway was constructed, expression level heterologous enzyme optimized promoter engineering. Next, biosynthetic modified rational modular strategies further enhance production. Subsequently, transcription factor TorI identified negatively regulate biosynthesis based on transcriptomic data. Finally, dynamic toggle switch controlling tyrA gene designed reconstruct Without additional supplements l-tyrosine, final strain AT25 generated highest ever reported among inducer-free, strains, producing 30.52 g/L productivity 0.51 g/L/h. systematic demonstrated this study can provide reference establishing factories together other compounds.

Language: Английский

Lysophosphatidylcholine induces ceramide synthase-2 expression in primary culture model of astrocytopathy: potential therapeutic target in multiple sclerosis DOI
Abhipsa Mohapatra,

Amarjeet Amarjeet,

Bhaskaranand Pancholi

et al.

Molecular Biology Reports, Journal Year: 2025, Volume and Issue: 52(1)

Published: Jan. 28, 2025

Language: Английский

Citations

0
Promoting remyelination in central nervous system diseases: potentials and prospects of natural products and herbal medicine DOI Creative Commons
Hongru Zhu, En Hu, Xin Guo

et al.

Pharmacological Research, Journal Year: 2024, Volume and Issue: unknown, P. 107533 - 107533

Published: Nov. 1, 2024

Language: Английский

Citations

2
Antioxidant Therapies in the Treatment of Multiple Sclerosis DOI Creative Commons
Félix Javier Jiménez‐Jiménez, Hortensia Alonso‐Navarro, Paula Salgado-Cámara

et al.

Biomolecules, Journal Year: 2024, Volume and Issue: 14(10), P. 1266 - 1266

Published: Oct. 8, 2024

Several studies have proposed a potential role for oxidative stress in the development of multiple sclerosis (MS). For this reason, it seems tentative to think that treatment with antioxidant substances could be useful disease. In narrative review, we provide summary current findings on treatments, both experimental models MS, especially autoimmune encephalomyelitis (EAE) and cuprizone-induced demyelination model, clinical trials patients diagnosed MS. Practically all antioxidants tested MS shown improvement parameters, delaying evolution disease, improving histological biochemical including decreased levels markers inflammation central nervous system other tissues. Only few been carried out investigate efficacy most them short term involving series patients, so results these should considered inconclusive. regard, would desirable design long-term, randomized, multicenter long assessing several demonstrated

Language: Английский

Citations

1
Development of a β-Arbutin Hyperproducer in Escherichia coli by Rational Modular and Transcription Factor Engineering DOI

Shuran Jiang,

Xuewei Pan, Xinyu Zhang

et al.

ACS Sustainable Chemistry & Engineering, Journal Year: 2024, Volume and Issue: 12(52), P. 18588 - 18599

Published: Dec. 18, 2024

β-Arbutin is a valuable aromatic compound with multiple biological activities. The exploitation of efficient engineering microbial platforms for β-arbutin production has been an appealing research avenue in recent years due to l-tyrosine auxotrophy and the limited distribution metabolic flux. Herein, inducer-free nonauxotrophic cell factory successfully constructed Escherichia coli toward high-titer by combining strategies. First, de novo synthesis pathway was constructed, expression level heterologous enzyme optimized promoter engineering. Next, biosynthetic modified rational modular strategies further enhance production. Subsequently, transcription factor TorI identified negatively regulate biosynthesis based on transcriptomic data. Finally, dynamic toggle switch controlling tyrA gene designed reconstruct Without additional supplements l-tyrosine, final strain AT25 generated highest ever reported among inducer-free, strains, producing 30.52 g/L productivity 0.51 g/L/h. systematic demonstrated this study can provide reference establishing factories together other compounds.

Language: Английский

Citations

0