N6-Methyladenosine (m6A)-Circular RNA Pappalysin 1 (circPAPPA) from Cashmere Goats: Identification, Regulatory Network and Expression Potentially Regulated by Methylation in Secondary Hair Follicles Within the First Intron of Its Host Gene

Animals, Journal Year: 2025, Volume and Issue: 15(4), P. 581 - 581

Published: Feb. 18, 2025

N6-methyladenosine (m6A) is one of the most abundant modifications in eukaryotic RNA molecules and mediates functional exertion molecules. We characterized circPAPPA validated its potential m6A modification sites secondary hair follicles (SHFs) cashmere goats. Furthermore, we generated integrated regulatory networks along with enrichment analysis signaling pathways. also explored relationship expression first intron methylation PAPPA gene SHFs Host source revealed that derived from complete exon 2 gene, spliced reverse orientation, predominantly localized cytoplasm SHF stem cells The was verified to contain at least four goats, including m6A-450/456, m6A-852, m6A-900, m6A-963. network indicated complex diverse relationships m6A-circPAPPA putative molecules, miRNAs, mRNAs, proteins. Enrichment pathways showed might play multiple roles growth development goats through mediated by target miRNAs likely significantly involved dynamic goat SHFs. Results this study provided novel information elucidate biological fiber.

Language: Английский

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Non-coding RNA-mediated granulosa cell dysfunction during ovarian aging: from mechanisms to potential interventions

Non-coding RNA Research, Journal Year: 2025, Volume and Issue: 12, P. 102 - 115

Published: March 4, 2025

As the earliest aging organ in reproductive system, ovary has both and endocrine functions, which are closely related to overall female health. The exact pathogenesis of ovarian (OA) remains incompletely understood, with granulosa cells (GCs) dysfunction playing a significant role this process. Recent advancements research biotechnology have highlighted importance non-coding RNAs (ncRNAs), including micro RNAs, long circular regulating biological functions GCs through gene expression modulation. This paper provides comprehensive overview ncRNAs various cellular such as apoptosis, autophagy, proliferation, steroid synthesis GCs, explores underlying regulatory mechanisms. Additionally, therapeutic potential ncRNAs, particularly those carried by exosomes derived from mesenchymal stem cells, delaying OA is discussed. Understanding mechanisms GC function current progress field crucial for identifying effective biomarkers targets, ultimately aiding early diagnosis, prognostic assessment, individualized treatment OA.

Language: Английский

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CircPAPPA binds to PAPPA and promotes trophoblast proliferation in spontaneous abortion through the formation of circPAPPA-PAPPA complexes

Gene, Journal Year: 2025, Volume and Issue: 960, P. 149538 - 149538

Published: May 1, 2025

Spontaneous abortion (SA) is one of the most common complications early pregnancy. Circular RNAs play an important regulatory role in development pregnancy-associated diseases females. While PAPPA known to be lower several problematic pregnancy conditions, specific it plays spontaneous has not yet been clearly defined. High-throughput sequencing and bioinformatics analysis were used sort circRNA expression profile villus tissues from abortions that happened on their own. The presence circPAPPA trophoblasts detected by RNase-R digestion RNA-FISH. effect proliferation was evaluated transfecting plasmids siRNAs into cells, followed assessing impact through CCK-8 assay. Additionally, we investigated localization binding protein RNA FISH/IF, RIP pull-down assays. We identified characterized a new circRNA, circPAPPA, whose down-regulated abortion. And high levels lead increased trophoblast vitro. CircPAPPA can bind protein; mechanistically, significantly influences how regulates activity. Altogether, our study shows binds PAPPA, enhancing vitro, suggests targeting circPAPPA-PAPPA interaction might novel therapeutic strategy for addressing

Language: Английский

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Viroids and Retrozymes: Plant Circular RNAs Capable of Autonomous Replication

Plants, Journal Year: 2024, Volume and Issue: 14(1), P. 61 - 61

Published: Dec. 27, 2024

Among the long non-coding RNAs that are currently recognized as important regulatory molecules influencing a plethora of processes in eukaryotic cells, circular (circRNAs) represent distinct class predominantly produced by back-splicing pre-mRNA. The most studied mechanisms involving circRNAs acting miRNA sponges, forming R-loops with genomic DNA, and encoding functional proteins. In addition to generated back-splicing, two types capable autonomous RNA-RNA replication systemic transport have been described plants: viroids, which infectious cause number plant diseases, retrozymes, transcripts retrotransposon loci circularization due ribozymes. Based on common features, viroids retrozymes considered be evolutionarily related. Here, we provide an overview biogenesis functions non-replicating further discuss detail available data focusing their structural mechanisms, interaction cellular components, plants. addition, biotechnological approaches replication-capable discussed, well potential applications research agriculture.

Language: Английский

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