Novel Eel Skin Fibroblast Cell Line: Bridging Adherent and Suspension Growth for Aquatic Applications Including Virus Susceptibility DOI Creative Commons
Zaiyu Zheng, Bin Chen, Xiaodong Liu

et al.

Biology, Journal Year: 2024, Volume and Issue: 13(12), P. 1068 - 1068

Published: Dec. 20, 2024

Suspension growth can greatly increase the cell density and yield of metabolites. To meet requirements aquatic industries, a culture model derived from Anguilla anguilla skin was developed using explant outgrowth enzyme-digesting passaging methods. These cells were kept in vitro continuously for over 12 months subcultured 68 times. This heteroploid line, designated as ES, naturally adapt to adherent suspension reversibly under certain temperatures, serum percentages, inoculum densities, without need any microcarriers or special medium additives. The ES continue being highly productive temperature range 15–37 °C percentage ranging 3 15%. An higher than 5 × 105 cells·mL−1 is necessary turn into efficiently. green fluorescent protein (GFP) reporter gene successfully expressed cells. demonstrated susceptibility Anguillid herpesvirus (AngHV) red-spotted grouper nervous necrosis virus (RGNNV). first natural origin; it does not require processes domestication carrier dissolution, making promising cost-effective vaccine production, bio-pharmaceutical manufacturing, cellular agriculture.

Language: Английский

Improve your success with fish cell lines—small things that matter DOI Creative Commons
Anita Solhaug, Georgina C. Dowd, Vivian R. Dayeh

et al.

In Vitro Cellular & Developmental Biology - Animal, Journal Year: 2025, Volume and Issue: unknown

Published: April 9, 2025

Abstract There is a drive towards reducing animal experiments and developing robust biologically relevant in vitro models based on cell lines, including those derived from fish. At the time of writing, Cellosaurus, knowledge base current lines used research, listed more than 900 fish its database. One key challenges facing biology lack fundamental technical information regarding isolation, culture, application lines. Researchers often work silos, encountering similar challenges, each spending significant resources overcoming same issues for which solutions may not be readily accessible. Here, we share some considerations maintenance, toxicology, have encountered over our collective decades experience.

Language: Английский

Citations

0
Decoding ex vivo tissue-like growth of fish vs mammalian cells as scalable cell-based food DOI

Jing Yu,

Qian Lu,

Rongli Niu

et al.

Trends in Food Science & Technology, Journal Year: 2025, Volume and Issue: unknown, P. 105019 - 105019

Published: April 1, 2025

Language: Английский

Citations

0
Establishment of a New Endothelial Cell Line of Japanese Eel and Its Application to the Investigation of Temperature Preference of Japanese Eel Endothelial Cells‐Infecting Virus DOI
Kousuke Umeda, Tomokazu Takano,

Norio Iinuma

et al.

Journal of Fish Diseases, Journal Year: 2025, Volume and Issue: unknown

Published: April 20, 2025

ABSTRACT Japanese eel endothelial cells‐infecting virus (JEECV) is the causative agent of viral cell necrosis (VECNE), which adversely affects aquaculture in East Asian countries. The primarily infects vascular cells and causes enlargement nuclei. Notably, high water temperatures (approximately 35°C) reduce mortality from VECNE, but temperature preference this not known. In study, we established a line, designated JEE‐2, bulbus arteriosus ( Anguilla japonica ). JEE‐2 was considered because its morphology cholesterol uptake activity. JEECV successfully cultured cells. vitro ‐ caused cytopathic effects, such as enlarged nuclei VECNE symptoms vivo. We examined preferences vitro. showed highest growth at 25°C–30°C, relatively slower 35°C, slight 37°C. grew 25°C–32.5°C, considerably limited 35°C. These results explain lower eels with temperatures. This study will help further understand infection mechanism develop control measures against it.

Language: Английский

Citations

0
Novel Eel Skin Fibroblast Cell Line: Bridging Adherent and Suspension Growth for Aquatic Applications Including Virus Susceptibility DOI Creative Commons
Zaiyu Zheng, Bin Chen, Xiaodong Liu

et al.

Biology, Journal Year: 2024, Volume and Issue: 13(12), P. 1068 - 1068

Published: Dec. 20, 2024

Suspension growth can greatly increase the cell density and yield of metabolites. To meet requirements aquatic industries, a culture model derived from Anguilla anguilla skin was developed using explant outgrowth enzyme-digesting passaging methods. These cells were kept in vitro continuously for over 12 months subcultured 68 times. This heteroploid line, designated as ES, naturally adapt to adherent suspension reversibly under certain temperatures, serum percentages, inoculum densities, without need any microcarriers or special medium additives. The ES continue being highly productive temperature range 15–37 °C percentage ranging 3 15%. An higher than 5 × 105 cells·mL−1 is necessary turn into efficiently. green fluorescent protein (GFP) reporter gene successfully expressed cells. demonstrated susceptibility Anguillid herpesvirus (AngHV) red-spotted grouper nervous necrosis virus (RGNNV). first natural origin; it does not require processes domestication carrier dissolution, making promising cost-effective vaccine production, bio-pharmaceutical manufacturing, cellular agriculture.

Language: Английский

Citations

0