One pot PCR genotyping of ApoE for diagnosing Alzheimer’s disease DOI
Hye Min Kim,

Junu Bae,

Mihwa Yang

et al.

Published: May 5, 2025

Abstract Apolipoprotein E (apoE) genotyping is a valuable tool for assessing genetic risk associated with Alzheimer’s disease (AD). The ε4 allele strongly linked to an increased and earlier onset of AD symptoms. Individuals carrying one (heterozygous) or two (homozygous) copies the are at significantly higher compared those without it. In contrast, ε2 may offer protective effect, reducing likelihood developing disease. Many studies have reported use PCR-based methods apoE genotyping. However, current approaches typically require least tubes—and often more—to distinguish among six possible genotypes using mixtures allele-specific primers. Consolidating assay into single tube can substantially reduce time, minimize manual labor, increase throughput. To address this need, we developed one-pot PCR platform genotyping, utilizing RNA-DNA hybrid CataCleave probe. We provide detailed explanation assay’s principles, including its limit detection, specificity, resistance interference from other serum components, precision. Collectively, information enables researchers adopt our streamlined, single-tube method ease.

Language: Английский

MicroRNAs: A Novel Approach for Monitoring Treatment Response in Major Depressive Disorder? DOI Creative Commons
Cristina-Sorina Cătană, Monica Mihaela Marta, Daniel Ungureanu

et al.

Non-Coding RNA, Journal Year: 2025, Volume and Issue: 11(2), P. 21 - 21

Published: March 3, 2025

Major depressive disorder (MDD) is one of the most prevalent psychiatric disorders, with an increasing incidence each year and important socioeconomic burden. Although new treatments are continuously being developed, there no effective monitoring method to determine suitability treatment ensure positive outcomes. Therefore, patients often struggle ineffective antidepressants their potential adverse effects, which halts any future progress in managing disorder. Considering microRNAs (miRNAs) as biomarkers for various pathologies evidence modulation several genes involved MDD, this minireview aimed evaluate literature data on impact miRNAs MDD usefulness response. The correlations between expression support existence a common epigenetic mechanism explain differences influencing efficacy MDD.

Language: Английский

Citations

0
One pot PCR genotyping of ApoE for diagnosing Alzheimer’s disease DOI
Hye Min Kim,

Junu Bae,

Mihwa Yang

et al.

Published: May 5, 2025

Abstract Apolipoprotein E (apoE) genotyping is a valuable tool for assessing genetic risk associated with Alzheimer’s disease (AD). The ε4 allele strongly linked to an increased and earlier onset of AD symptoms. Individuals carrying one (heterozygous) or two (homozygous) copies the are at significantly higher compared those without it. In contrast, ε2 may offer protective effect, reducing likelihood developing disease. Many studies have reported use PCR-based methods apoE genotyping. However, current approaches typically require least tubes—and often more—to distinguish among six possible genotypes using mixtures allele-specific primers. Consolidating assay into single tube can substantially reduce time, minimize manual labor, increase throughput. To address this need, we developed one-pot PCR platform genotyping, utilizing RNA-DNA hybrid CataCleave probe. We provide detailed explanation assay’s principles, including its limit detection, specificity, resistance interference from other serum components, precision. Collectively, information enables researchers adopt our streamlined, single-tube method ease.

Language: Английский

Citations

0