Direct On-Chip Diagnostics of Streptococcus bovis/Streptococcus equinus Complex in Bovine Mastitis Using Bioinformatics-Driven Portable qPCR DOI Creative Commons

J.Y. Kim,

Eiseul Kim, Seung-Min Yang

et al.

Biomolecules, Journal Year: 2024, Volume and Issue: 14(12), P. 1624 - 1624

Published: Dec. 18, 2024

This study introduces an innovative on-site diagnostic method for rapidly detecting the Streptococcus bovis/Streptococcus equinus complex (SBSEC), crucial livestock health and food safety. Through a comprehensive genomic analysis of 206 genomes, this identified genetic markers that improved classification addressed misclassifications, particularly in genomes labeled S. lutetiensis. These were integrated into portable quantitative polymerase chain reaction (qPCR) can detect SBSEC species with high sensitivity (down to 101 or 100 colony-forming units/mL). The system featuring flat chip compact equipment allows immediate diagnosis within 30 min. was validated field conditions directly from cattle udders, farm environments, dairy products. Among samples, 51 tested positive bacteria associated mastitis. performance qPCR comparable laboratory methods, offering reliable alternative whole-genome sequencing early detection clinical, agricultural, environmental settings.

Language: Английский

Direct On-Chip Diagnostics of Streptococcus bovis/Streptococcus equinus Complex in Bovine Mastitis Using Bioinformatics-Driven Portable qPCR DOI Creative Commons

J.Y. Kim,

Eiseul Kim, Seung-Min Yang

et al.

Biomolecules, Journal Year: 2024, Volume and Issue: 14(12), P. 1624 - 1624

Published: Dec. 18, 2024

This study introduces an innovative on-site diagnostic method for rapidly detecting the Streptococcus bovis/Streptococcus equinus complex (SBSEC), crucial livestock health and food safety. Through a comprehensive genomic analysis of 206 genomes, this identified genetic markers that improved classification addressed misclassifications, particularly in genomes labeled S. lutetiensis. These were integrated into portable quantitative polymerase chain reaction (qPCR) can detect SBSEC species with high sensitivity (down to 101 or 100 colony-forming units/mL). The system featuring flat chip compact equipment allows immediate diagnosis within 30 min. was validated field conditions directly from cattle udders, farm environments, dairy products. Among samples, 51 tested positive bacteria associated mastitis. performance qPCR comparable laboratory methods, offering reliable alternative whole-genome sequencing early detection clinical, agricultural, environmental settings.

Language: Английский

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