Viruses, Journal Year: 2025, Volume and Issue: 17(4), P. 566 - 566
Published: April 14, 2025
Self-amplifying RNA is synthetic nucleic acid engineered to replicate within cells without generating viral particles. Derived from alphavirus genomes, saRNA retains the non-structural elements essential for replication while replacing structural with an antigen of interest. By enabling efficient intracellular amplification, offers a promising alternative conventional mRNA vaccines, enhancing expression requiring lower doses. However, this advantage comes challenges. In review, we highlight key limitations technology and explore potential strategies overcome them. identifying these challenges, aim provide insights that can guide future design saRNA-based therapeutics, extending their beyond vaccine applications.
Language: Английский