Use of ultrasound to improve the activity of cyclodextrin glycosyltransferase in the producing of β-cyclodextrins: Impact on enzyme activity, stability and insights into changes on enzyme macrostructure

Food Research International, Journal Year: 2024, Volume and Issue: 191, P. 114662 - 114662

Published: June 28, 2024

Language: Английский

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Enhanced performance of cyclodextrin glycosyltransferase by immobilization on amine-induced macroporous metal organic framework

LWT, Journal Year: 2024, Volume and Issue: 200, P. 116221 - 116221

Published: May 1, 2024

This study examined the effectiveness of a hierarchical copper-based metal–organic framework (H-Cu-BTC) in comparison with its microporous counterpart (Cu-BTC) for immobilization cyclodextrin glycosyltransferase (CGTase) use production. The adsorption capacity, conformational changes, and operational stability immobilized enzymes were examined. presence both macropores micropores proposed H-Cu-BTC resulted an enhanced maximum capacity 49.5 mg/g CGTase as compared to 30.6 Cu-BTC, which contains only micropores. was also shown more favorably affect secondary structure enzyme. Using H-Cu-BTC, proportion β-sheets, form major protein are responsible enzyme's stability, increase from 56% free 76.1% after immobilization. However, when using β-sheets decreased 44.1%. favorable surface attachment reflected better reusability, wherein activity preserved up 87% original ten repeated cycles reuse, 70% Cu-BTC. successful on demonstrated that it could be used robust biocatalyst conversion starchy waste into cyclodextrins.

Language: Английский

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Metal–organic frameworks as highly effective platforms for enzyme immobilization–current developments and future perspectives

Brazilian Journal of Chemical Engineering, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 18, 2024

Abstract This study presents a thorough overview of metal–organic frameworks’ (MOFs) utility as supports for enzyme immobilization. It details bibliometric analysis the field from 2014–2023, based on 911 Web Science articles. Research outlines functional properties MOFs, groups, and common immobilization techniques. showcases diverse enzyme-MOF combinations numerous applications, including industrial biocatalysis, medical diagnostics, drug development, biomass conversion, environmental protection, biosensor creation. Current development trends future directions are identified through literature analysis. Latent Dirichlet Allocation (LDA) modeling revealed key research themes over years, highlighting shifts in focus towards advanced catalytic methods adsorption Additionally, word cloud generated 2024 publications indicates an increasing emphasis specialized applications involving ZIFs, nanoparticles, magnetic structures. Key perspectives include new MOF materials, such biologically derived core–shell hierarchical porous nanoMOFs, mesoporous MOFs. Novel materials inspired by carriers, covalent organic frameworks (COFs) aerogels (MOAs), also highlighted. Lastly, emphasizes potential multienzymatic systems multifunctional MOF-enzyme composites, facilitating multiple functionalities within single system.

Language: Английский

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Uso da tecnologia de ultrassom no processamento da ciclodextrina glicosiltransferase: efeito na atividade, estabilidade e macroestrutura

Published: Feb. 22, 2024

As maltodextrinas podem ser convertidas em β-ciclodextrina (β-CD) através da ciclodextrina glicosiltransferase (CGTase). No entanto, a CGTase apresenta limitações devido aos altos custos de produção e à baixa estabilidade enzimática na β-CD. Este estudo explora tecnologia ultrassom (US) como uma solução potencial para potencializar performance enzimática. Investigando o impacto do US atividade, conformação estrutural CGTase, os experimentos utilizaram um banho ultrassônico (25 kHz, 38 W/L) com pH variado (4,0 6,0), temperaturas (25, 55, 80°C), tempos (5-120 min). Os resultados revelaram que pré-tratamento aumentou significativamente atividade CGTase. Notavelmente, 6,0, foram observados aumentos máximos 93% (pré-tratamento 25°C/30min), 68% 25°C/30min) 31% 25°C/60min) 25°C, 55°C 80°C, respectivamente. Da mesma forma, 4,0 durante 5 min/55°C mostrou relativa aumentada 28%, 21% 16% 25, 55 80°C. níveis aumentados permaneceram estáveis após 24 horas armazenamento 8°C. pré-tratamentos ultrassônicos induziram alterações conformacionais evidentes aumento até 11% intensidade fluorescência intrínseca, resultaram modificações macroestruturais, incluindo redução tamanho partícula (até 85%) índice polidispersão (45,8%). Estes são interessantes demonstram sob condições específicas sonicação (pH, tempo temperatura) é possível ativar produzircompostos tecnologiaemergente; industrialmente relevantes. Palavras-chave: glicosiltransferase; enzimática; sonicação; β-ciclodextrina.

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Bio-activation and mathematical modeling of ZIF-L encapsulated with laccase for enhanced ibuprofen removal from wastewater

Cleaner Engineering and Technology, Journal Year: 2024, Volume and Issue: unknown, P. 100875 - 100875

Published: Dec. 1, 2024

Language: Английский

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