Retinal Müller Cell-Released Exosomal MiR-92a-3p Delivers Interleukin-17A Signal by Targeting Notch-1 to Promote Diabetic Retinopathy

Investigative Ophthalmology & Visual Science, Journal Year: 2025, Volume and Issue: 66(1), P. 1 - 1

Published: Jan. 2, 2025

Purpose: Inflammatory processes have been involved in diabetic retinopathy (DR). Interleukin (IL)-17A, a pro-inflammatory cytokine, is associated with DR occurrence and development. However, mechanisms underlying the IL-17A impact on need further investigations. Herein, we show that exosomal miRNA delivers signal from Müller cells (MCs) to retinal ganglion (RGCs) facilitate DR. Methods: Exosomes isolated primarily cultured MCs were identified high-throughput sequencing was used indicate differentially expressed miRNAs exosomes. Targeting relationship of miR-92a-3p Notch-1 determined by dual-luciferase reporter assay. treated high glucose (HG), IL-17A, anti-IL-17A antibody, inhibitor, or/and mimic, exosomes derived various treatments applied primary RGCs. mice induced streptozotocin (STZ) subjected intravitreal injection. Results: Expression treatment either absence or presence HG upregulated, effect blocked antibody. The IL-17A-treated downregulated expression recipient RGCs increased neuronal death. These effects suppressed inhibitor but enhanced mimic. In STZ mice, administration RGC apoptosis. hindered inhibitor. Conclusions: MC-released transmits inhibiting target accelerate progression. An containing may become potential therapeutic strategy for

Language: Английский

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Natural remedies proposed for the management of diabetic retinopathy (DR): diabetic complications

Naunyn-Schmiedeberg s Archives of Pharmacology, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 15, 2025

Abstract Diabetic retinopathy (DR) represents a significant and serious complication associated with diabetes mellitus (DM), often resulting in considerable visual impairment or even blindness. The intricate pathological processes underlying DR complicate the effectiveness of current treatment modalities. Studies have highlighted potential natural products via several beneficial effects including anti-inflammatory, antioxidant, anti-neovascular, anti-apoptotic properties. Flavonoids, saponins, saccharides, alkaloids exhibited various vivo vitro studies. However, clinical utilization these compounds is hindered by issues such as inadequate specificity, low bioavailability, toxicity. Therefore, there pressing need for rigorous studies to confirm efficacy preventing mitigating progression DR.

Language: Английский

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Systematic Inflammation and Oxidative Stress Elevation in Diabetic Retinopathy and Diabetic Patients with Macular Edema

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(8), P. 3810 - 3810

Published: April 17, 2025

This study investigates the association between diabetic retinopathy (DR) and its complication, macular edema (DME), compared it with biomarkers of oxidative stress. aimed to compare main indicators development measured as parameters stress lipid oxidation, DNA damage, cytokine levels monitor their quantitative manifestation in DME. evaluated 134 patients (62 males 72 females; aged 62.10 ± 11.22 years) divided them into two groups: type 2 diabetes mellitus DR All results were healthy volunteers (n = 94) showed that DME had significantly higher ROS, production, damage. In addition, decreased nitric oxide (NO) an impaired NO synthase (NOS) system (p < 0.05). These findings suggest are unable compensate for high Reduced may be due availability. highlights compromised status a contributing factor decompensated mellitus. An assessment inflammatory aid early detection prediction complications.

Language: Английский

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Mechanism and Therapeutic Targets of Circulating Immune Cells in Diabetic Retinopathy

Pharmacological Research, Journal Year: 2024, Volume and Issue: 210, P. 107505 - 107505

Published: Nov. 14, 2024

Diabetic retinopathy (DR) continues to be the leading cause of preventable vision loss among working-aged adults, marked by immune dysregulation within retinal microenvironment. Typically, retina is considered as an immune-privileged organ, where circulating cells are restricted from entry under normal conditions. However, during progression DR, this privilege compromised breach barrier and infiltrate retina. Increasing evidence suggests that vascular neuronal degeneration in DR largely driven infiltration cells, particularly neutrophils, monocyte-derived macrophages, lymphocytes. This review delves into mechanisms therapeutic targets associated with these cell populations offering a promising innovative approach managing disease.

Language: Английский

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Roles of noncoding RNAs in diabetic retinopathy: Mechanisms and therapeutic implications

Life Sciences, Journal Year: 2024, Volume and Issue: unknown, P. 123092 - 123092

Published: Oct. 1, 2024

Language: Английский

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