Harnessing the Microbiome: CRISPR-Based Gene Editing and Antimicrobial Peptides in Combating Antibiotic Resistance and Cancer DOI Creative Commons
Radwa Abdallnasser Amen,

Yahaya Hassan,

Rawan A Essmat

et al.

Probiotics and Antimicrobial Proteins, Journal Year: 2025, Volume and Issue: unknown

Published: May 16, 2025

Language: Английский

Beyond the Hayflick limit: How microbes influence cellular aging DOI
Mohammad Abavisani,

Saba Faraji,

Negar Ebadpour

et al.

Ageing Research Reviews, Journal Year: 2025, Volume and Issue: 104, P. 102657 - 102657

Published: Jan. 7, 2025

Language: Английский

Citations

3
Reinvigorating AMR resilience: leveraging CRISPR–Cas technology potentials to combat the 2024 WHO bacterial priority pathogens for enhanced global health security—a systematic review DOI Creative Commons
Olalekan John Okesanya, Mohamed Mustaf Ahmed, Jerico Bautista Ogaya

et al.

Tropical Medicine and Health, Journal Year: 2025, Volume and Issue: 53(1)

Published: April 2, 2025

Abstract Background Antimicrobial resistance (AMR) poses a global health threat, particularly in low- and middle-income countries (LMICs). Clustered regularly interspaced short palindromic repeats (CRISPR)–Cas system technology offers promising tool to combat AMR by targeting disabling genes WHO bacterial priority pathogens. Thus, we systematically reviewed the potential of CRISPR–Cas address AMR. Methods This systematic review adhered Preferred Reporting Items for Systematic Reviews Meta-Analyses (PRISMA) guidelines. A comprehensive literature search was conducted using Scopus PubMed databases, focusing on publications from 2014 June 2024. Keywords included “CRISPR/Cas,” “antimicrobial resistance,” “pathogen.” The eligibility criteria required original studies involving CRISPR/Cas systems that targeted Data were extracted eligible studies, qualitatively synthesized, assessed bias Joanna Briggs Institute (JBI)-standardized tool. Results 48 revealed diverse systems, including CRISPR–Cas9, CRISPR–Cas12a, CRISPR–Cas3, various genes, such as blaOXA-232, blaNDM, blaCTX-M, ermB, vanA, mecA , fosA3 blaKPC mcr-1, which are responsible carbapenem, cephalosporin, methicillin, macrolide, vancomycin, colistin, fosfomycin resistance. Some have explored role CRISPR virulence gene suppression, enterotoxin tsst1 iutA Staphylococcus aureus Klebsiella pneumoniae . Delivery mechanisms include bacteriophages, nanoparticles, electro-transformation, conjugative plasmids, demonstrate high efficiency vitro vivo. CRISPR-based diagnostic applications demonstrated sensitivity specificity, with detection limits low 2.7 × 10 2 CFU/mL, significantly outperforming conventional methods. Experimental reported significant reductions resistant populations complete suppression strains. Engineered phagemid particles plasmid-curing been shown eliminate IncF cured plasmids carrying vanA mcr-1 blaNDM 94% efficiency, restore antibiotic susceptibility. Gene re-sensitization strategies used susceptibility E. coli blaKPC-2-mediated carbapenem MDR bacteria. Whole-genome sequencing bioinformatics tools provided deeper insights into CRISPR-mediated defense mechanisms. Optimization enhanced gene-editing efficiencies, offering approach tackling high-priority Conclusions has across While promising, challenges optimizing vivo delivery, mitigating resistance, navigating ethical-regulatory barriers must be addressed facilitate clinical translation.

Language: Английский

Citations

1
CRISPR in medical microbiology – the try and trial towards future DOI
Manikandan Alagumuthu,

Joe Jacob Kunju,

J. Immanuel Suresh

et al.

Next research., Journal Year: 2025, Volume and Issue: unknown, P. 100276 - 100276

Published: March 1, 2025

Language: Английский

Citations

0
CRISPR revolution: Unleashing precision pathogen detection to safeguard public health and food safety DOI
Jacob Tizhe Liberty, Sabri Bromage,

Endurance Peter

et al.

Methods, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

Language: Английский

Citations

0
Harnessing the Microbiome: CRISPR-Based Gene Editing and Antimicrobial Peptides in Combating Antibiotic Resistance and Cancer DOI Creative Commons
Radwa Abdallnasser Amen,

Yahaya Hassan,

Rawan A Essmat

et al.

Probiotics and Antimicrobial Proteins, Journal Year: 2025, Volume and Issue: unknown

Published: May 16, 2025

Language: Английский

Citations

0