Harnessing lactic acid bacteria for nicotinamide mononucleotide biosynthesis: a review of strategies and future directions DOI Creative Commons

Linghui Kong,

Xinyu Li, Taiyu Liu

et al.

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: Dec. 13, 2024

Nicotinamide mononucleotide (NMN), one of the crucial precursors nicotinamide adenine dinucleotide, has garnered considerable interest for its pharmacological and anti-aging effects, conferring potential health economic benefits humans. Lactic acid bacteria (LAB) are most important probiotics, which is commonly used in dairy industry. Due to probiotic properties, it presents an attractive platform food-grade NMN production. LAB have also been extensively utilized enhance functional properties pharmaceuticals cosmetics, making them promising candidates large-scale up synthesis NMN. This review provides in-depth analysis various metabolic engineering strategies, including enzyme optimization, pathway rewiring, fermentation process enhancements, increase yields LAB. It explores both CRISPR/Cas9 traditional methods manipulate key biosynthetic pathways. In particular, this study discussed future research directions, emphasizing application synthetic biology, systems AI-driven optimization further invaluable insights into developing scalable industrially relevant processes production meet growing market demand.

Language: Английский

Use of Nicotinamide Mononucleotide as Non-Natural Cofactor DOI Open Access
Tahseena Naaz, Beom Soo Kim

Catalysts, Journal Year: 2025, Volume and Issue: 15(1), P. 37 - 37

Published: Jan. 3, 2025

Nicotinamide mononucleotide (NMN) has emerged as a promising non-natural cofactor with significant potential to transform biocatalysis, synthetic biology, and therapeutic applications. By modulating NAD⁺ metabolism, NMN offers unique advantages in enzymatic reactions, metabolic engineering, regenerative medicine. This review provides comprehensive analysis of NMN’s biochemical properties, mechanisms action, diverse Emphasis is placed on its role addressing challenges multi-enzyme cascades, biofuel production, the synthesis high-value chemicals. The paper also highlights critical research gaps, including need for scalable methods, improved integration into systems, toxicity studies use. Emerging technologies such AI-driven enzyme design CRISPR-based genome engineering are discussed transformative tools optimizing NMN-dependent pathways. Furthermore, synergistic biology innovations, cell-free systems dynamic regulatory networks, explored, paving way precise modular biotechnological solutions. Looking forward, versatility positions it pivotal tool advancing sustainable bioprocessing precision Addressing current limitations through interdisciplinary approaches will enable redefine boundaries innovation. serves roadmap leveraging across scientific industrial domains.

Language: Английский

Citations

0
An overview of engineering microbial production of nicotinamide adenine mononucleotide DOI

Baoxian Li,

Xiangfeng Meng, Weifeng Liu

et al.

Journal of Biotechnology, Journal Year: 2024, Volume and Issue: 396, P. 80 - 88

Published: Nov. 2, 2024

Language: Английский

Citations

1
Systematic Engineering for High-level Production of β-nicotinamide mononucleotide from NAM and Ribose DOI Creative Commons
T. W. Bao, P.S. Weng, Jingfang Wang

et al.

Food Bioscience, Journal Year: 2024, Volume and Issue: unknown, P. 105725 - 105725

Published: Dec. 1, 2024

Language: Английский

Citations

1
Harnessing lactic acid bacteria for nicotinamide mononucleotide biosynthesis: a review of strategies and future directions DOI Creative Commons

Linghui Kong,

Xinyu Li, Taiyu Liu

et al.

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: Dec. 13, 2024

Nicotinamide mononucleotide (NMN), one of the crucial precursors nicotinamide adenine dinucleotide, has garnered considerable interest for its pharmacological and anti-aging effects, conferring potential health economic benefits humans. Lactic acid bacteria (LAB) are most important probiotics, which is commonly used in dairy industry. Due to probiotic properties, it presents an attractive platform food-grade NMN production. LAB have also been extensively utilized enhance functional properties pharmaceuticals cosmetics, making them promising candidates large-scale up synthesis NMN. This review provides in-depth analysis various metabolic engineering strategies, including enzyme optimization, pathway rewiring, fermentation process enhancements, increase yields LAB. It explores both CRISPR/Cas9 traditional methods manipulate key biosynthetic pathways. In particular, this study discussed future research directions, emphasizing application synthetic biology, systems AI-driven optimization further invaluable insights into developing scalable industrially relevant processes production meet growing market demand.

Language: Английский

Citations

0