Developing fibrin-based biomaterials/scaffolds in tissue engineering

Bioactive Materials, Journal Year: 2024, Volume and Issue: 40, P. 597 - 623

Published: Aug. 15, 2024

Tissue engineering technology has advanced rapidly in recent years, offering opportunities to construct biologically active tissues or organ substitutes repair even enhance the functions of diseased and organs. Tissue-engineered scaffolds rebuild extracellular microenvironment by mimicking matrix. Fibrin-based possess numerous advantages, including hemostasis, high biocompatibility, good degradability. Fibrin provide an initial matrix that facilitates cell migration, differentiation, proliferation, adhesion, also play a critical role cell-matrix interactions. are now widely recognized as key component tissue engineering, where they can facilitate defect repair. This review introduces properties fibrin, its composition, structure, biology. In addition, modification cross-linking modes fibrin discussed, along with various forms commonly used engineering. We describe biofunctionalization fibrin. provides detailed overview use applications skin, bone, nervous tissues, novel insights into future research directions for clinical treatment.

Language: Английский

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Nerve‐Derived Extracellular Matrix Promotes Neural Differentiation of Bone Marrow Stromal Cells and Enhances Interleukin‐4 Efficacy for Advanced Nerve Regeneration

Advanced Healthcare Materials, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 16, 2025

Facilitating neuronal differentiation of stem cells and microenvironment remodeling are the key challenges in cell-based transplantation strategies for central nervous system regeneration. Herein, study harnesses intrinsic pro-neural potential nerve-derived extracellular matrix (NDEM) its specific affinity cytokines to develop an NDEM-gelatin methacryloyl(gelMA)-based bifunctional hydrogel delivery cytokines. This promotes neural bone marrow stromal (BMSCs) optimizes therapeutic index Interleukin-4 (IL-4) spinal cord injury (SCI) treatment. It is observed that incorporating NDEM into intrinsically BMSC neuron-like effectively regulates IL-4 release kinetics match reconstructing timeframe. Further analysis reveals trace amounts endogenous basic fibroblast growth factor (bFGF) detected exhibit a potent effect promoting differentiation. The sustained from significantly encourages macrophage polarization toward M2 phase, optimizing transplant throughout reconstruction process. demonstrates NDEM-based optimization strategy hybrid achieve synchronized regenerative medicine applications.

Language: Английский

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Bridging the Gap: Contemporary Paradigms in Nerve Scaffolds and Regeneration

Advances in Wound Care, Journal Year: 2025, Volume and Issue: unknown

Published: March 11, 2025

Peripheral nerve injuries, especially those with complete transection of major nerves, create significant morbidity including debilitating pain, loss protective haptic feedback, and impaired volitional control musculature. The societal burden cost medical care for these injuries are enormous, estimates in the United States alone excess $670 million per year. In clinical scenarios a segmental gap where end-to-end coaptation without tension is not possible, "bridge" or scaffold must be interposed to facilitate communication between proximal distal stumps organized growth following Wallerian degeneration. A multitude constructs have been created studied this regeneration. Among three overall types bridge employed contemporary care-conduit/scaffold, allograft, autograft-each has downsides ranging from limited successful ingrowth donor site morbidity. Despite tremendous work over last 150 years biology technology treatment peripheral injury, biological processes governing regeneration remain incompletely understood. Especially cases long gaps, there remains room improvement. Ongoing studies identified several promising modalities scaffolds more efficient effective neuronal outgrowth but still require further investigation. Here, we review paradigms interposing reexamine physiology, regulatory programs regeneration, strategic targets neurogenic pathways that may novel modalities.

Language: Английский

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Hybrid construction of tissue-engineered nerve graft using skin derived precursors induced neurons and Schwann cells to enhance peripheral neuroregeneration

Materials Today Bio, Journal Year: 2024, Volume and Issue: 28, P. 101196 - 101196

Published: Aug. 9, 2024

Peripheral nerve injury is a major challenge in clinical treatment due to the limited intrinsic capacity for regeneration. Tissue engineering approaches offer promising solutions by providing biomimetic scaffolds and cell sources promote In present work, we investigated potential role of skin-derived progenitors (SKPs), which are induced into neurons Schwann cells (SCs), their extracellular matrix tissue-engineered grafts (TENGs) enhance peripheral neuroregeneration. SKPs were differentiate SCs

Language: Английский

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Youthful Stem Cell Microenvironments: Rejuvenating Aged Bone Repair Through Mitochondrial Homeostasis Remodeling

Advanced Science, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 17, 2025

Extracellular matrix (ECM) derived from mesenchymal stem cells regulates antioxidant properties and bone metabolism by providing a favorable extracellular microenvironment. However, its functional role molecular mechanism in mitochondrial function regulation aged regeneration remain insufficiently elucidated. This proteomic analysis has revealed greater abundance of proteins supporting the young ECM (Y-ECM) secreted marrow-derived (BMMSCs) compared to (A-ECM). Further studies demonstrate that Y-ECM significantly rejuvenates energy adult BMMSCs (A-BMMSCs) through promotion respiratory functions amelioration oxidative stress. A-BMMSCs cultured on exhibited enhanced multi-lineage differentiation potentials vitro ectopic formation vivo. Mechanistically, silencing silent information regulator type 3 (SIRT3) gene abolished protective impact A-BMMSCs. Notably, novel composite biomaterial combining hyaluronic acid methacrylate hydrogel microspheres with is developed, which yielded substantial improvements healing defects an rat model. Collectively, these findings underscore pivotal maintaining redox homeostasis present promising therapeutic strategy for repair defects.

Language: Английский

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Bridging bioengineering and nanotechnology: Bone marrow derived mesenchymal stem cell-exosome solutions for peripheral nerve injury

World Journal of Stem Cells, Journal Year: 2025, Volume and Issue: 17(1)

Published: Jan. 17, 2025

Peripheral nerve injury (PNI) is a common disease that difficult to regeneration with current therapies. Fortunately, Zou et al demonstrated the role and mechanism of bone marrow derived mesenchymal stem cells (BMSCs) in promoting regeneration, revealing broad prospects for BMSCs transplantation alleviating PNI. We confirmed fact significantly alleviate PNI, but there are shortcomings such as low cell survival rate immune rejection, which limit wide application BMSCs. BMSCs-derived exosomes (Exos) considered promising cell-free nanomedicine avoiding ethical issues Exos combination bioengineering therapeutics (including extracellular matrix, hydrogel) brings new hope provides favorable microenvironment neurological restoration therapeutic strategy safety profile, increases expression neurotrophic factors, promotes axonal myelin demonstrates strong potential enhance neurogenesis. Therefore, engineered exhibit better properties, stronger targeting more beneficial components. This article briefly describes nanotechnology therapies proposes clinical challenges PNI improve efficacy treatment

Language: Английский

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Potentially commercializable nerve guidance conduits for peripheral nerve injury: Past, present, and future

Materials Today Bio, Journal Year: 2025, Volume and Issue: 31, P. 101503 - 101503

Published: Feb. 6, 2025

Peripheral nerve injuries are a prevalent global issue that has garnered great concern. Although autografts remain the preferred clinical approach to repair, their efficacy is hampered by factors like donor scarcity. The emergence of guidance conduits as novel tissue engineering tools offers promising alternative strategy. This review aims interpret and commercialization from both laboratory perspectives. To enhance comprehension situations, this article provides comprehensive analysis approved United States Food Drug Administration. It proposes initial six months post-transplantation critical window period for evaluating efficacy. Additionally, study conducts systematic discussion on research progress conduits, focusing biomaterials add-on strategies pivotal regeneration, supported literature analysis. conduit materials prospective optimal thoroughly discussed. strategies, together with distinct obstacles potentials deeply analyzed. Based above evaluations, development path manufacturing strategy envisioned. conclusion promoting summarized follows: 1) optimization fundamental means; 2) phased application additional emphasized direction; 3) additive techniques necessary tools. As result, findings provide academic practitioners valuable insights may facilitate future endeavors conduits.

Language: Английский

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The application of stem cells and exosomes in promoting nerve conduits for peripheral nerve repair

Biomaterials Research, Journal Year: 2025, Volume and Issue: 29

Published: Jan. 1, 2025

The repair of peripheral nerve injury (PNI) presents a multifaceted and protracted challenge, with current therapeutic approaches failing to achieve optimal outcomes, thereby not satisfying the considerable clinical demand. advent tissue engineering has led growing body experimental evidence indicating that synergistic application conduits, which provide structural guidance, alongside biological signals derived from exosomes stem cells, yields superior results for PNI compared isolated interventions. This combined approach holds great promise application. In this review, we present latest advancements in treatment through integration cells or conduits. We have addressed inadequate efficiency conjunction conduits 3 perspectives: enhancing exosomes, improving incorporating physical stimulation.

Language: Английский

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Bionic tissue engineering of peripheral nerves and progress in neuroregeneration

Chemical Engineering Journal, Journal Year: 2025, Volume and Issue: unknown, P. 162123 - 162123

Published: March 1, 2025

Language: Английский

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Effects of 3D-printed exosome-functionalized brain acellular matrix hydrogel on neuroinflammation in rats following cerebral hemorrhage

Stem Cell Research & Therapy, Journal Year: 2025, Volume and Issue: 16(1)

Published: April 20, 2025

Exosome-based therapeutics have garnered significant attention for intracerebral hemorrhage (ICH) treatment due to their capacity regulate metabolic dysregulation, restore cellular homeostasis, and modulate the injury microenvironment via bioactive cargoes such as microRNAs proteins. However, rapid systemic clearance enzymatic degradation critically limit therapeutic efficacy. To address this challenge, we engineered a three-dimensional (3D) bioprinted scaffold capable of encapsulating sustaining release human umbilical cord mesenchymal stem cell-derived exosomes (hUCMSC-exos). Based on previous research [1-3], was composed decellularized brain matrix (dECM), gelatin-methacryloyl (GelMA), silk fibroin (SF) crosslinked with photoinitiator. hUCMSC-exos were precisely incorporated extrusion-based 3D bioprinting. Release kinetics assessed in vitro elution vivo imaging. An ICH rat model received stereotaxic implantation exosome-laden (dECM@exo). Neuroinflammatory markers (IL-6, TNF-α, IL-10) apoptotic activity (JC-1, Annexin V/PI, TUNEL) quantified. Neurological outcomes longitudinally evaluated using modified Longa scale, Bederson scoring, sensorimotor tests (rotarod, forelimb placement) at 1, 4, 7 14 days post-ICH. dECM@exo demonstrated sustained exosome over days, significantly promoting neural tissue regeneration while attenuating perihematomal edema. Mechanistically, modulated pathological MMP inflammatory cytokine expression, thereby restoring extracellular homeostasis reducing neuronal apoptosis. The findings demonstrate that biological effectively maintains microenvironmental early stages improves associated condition. is poised serve robust platform drug delivery biotherapy treatment, offering viable alternative managing

Language: Английский

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