Advancement of Nanomaterials- and Biomaterials-Based Technologies for Wound Healing and Tissue Regenerative Applications

ACS Applied Bio Materials, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 28, 2025

Patients and healthcare systems face significant social financial challenges due to the increasing number of individuals with chronic external internal wounds that fail heal. The complexity healing process remains a serious health concern, despite effectiveness conventional wound dressings in promoting healing. Recent advancements materials science fabrication techniques have led development innovative enhance To further expedite process, novel approaches such as nanoparticles, 3D-printed dressings, biomolecule-infused emerged, along cell-based methods. Additionally, gene therapy technologies are being harnessed generate stem cell derivatives more functional, selective, responsive than their natural counterparts. This review highlights potential biomaterials, 3D bioprinting, gene- therapies However, it also underscores necessity for research address existing integrate these strategies into standard clinical practice.

Language: Английский

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Aspirin promotes odontogenic differentiation via a mechanism involving FOXC1, RUNX2, and MCAM expression

Journal of Oral Biosciences, Journal Year: 2025, Volume and Issue: unknown, P. 100622 - 100622

Published: Jan. 1, 2025

Language: Английский

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An HRL‐SC/HIF‐1α positive feedback loop enhances cell proliferation, migration and angiogenesis in dental pulp stem cells via PI3K/AKT signalling pathway

International Endodontic Journal, Journal Year: 2025, Volume and Issue: unknown

Published: March 28, 2025

Abstract Aim Dental pulp stem cells (DPSCs) are essential for regeneration but face low survival rates after transplantation. Genetic modification before transplantation is a promising solution to this issue. We aim elucidate the biological function and regulatory mechanism of hypoxic lncRNA HRL‐SC in DPSCs. Methodology The functions hypoxia inducible factor‐1α (HIF‐1α) DPSCs were evaluated vitro by cell proliferation, migration tube formation assays. Subcutaneous nude mice was used evaluate effect on DPSC viability vivo. RNA sequencing bioinformatics analysis, immunoprecipitation, dual luciferase reporter gene assay, co‐immunoprecipitation, fluorescence situ hybridization, immunofluorescence protein stability assays explore potential Data analysed one‐way analysis variance ( anova ) or Student's t ‐test, with p <.05 indicating statistical significance. Results HRL‐SC, hypoxia‐responsive lncRNA, enhanced abilities dental blocks revealed that HRL‐SC‐mediated exhibited improved elevated expression Ki‐67 CD31, along capacity form vascular‐like structures. HIF‐1α observed induce transcription HRL‐SC. Reciprocally, bound VHL, thereby inhibiting VHL‐mediated ubiquitination, which resulted positive feed‐forward loop HRL‐SC/HIF‐1α. RNA‐sequencing functional analyses closely associated hypoxia, angiogenesis, regeneration, integrin PI3K/AKT signalling pathways. Furthermore, shown stabilize ITGAV ITGB3 through PTBP1. Finally, it confirmed activated pathway via αvβ3/FAK HIF‐1α/PDK1 axes. Conclusions modified demonstrated signaling characteristics endothelial cells, may provide novel strategy application regeneration.

Language: Английский

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Guidance for evaluating biomaterials’ properties and biological potential for dental pulp tissue engineering and regeneration research

Dental Materials, Journal Year: 2024, Volume and Issue: 41(3), P. 248 - 264

Published: Dec. 13, 2024

Language: Английский

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