
Dental Materials, Journal Year: 2024, Volume and Issue: 41(3), P. 248 - 264
Published: Dec. 13, 2024
Language: Английский
Dental Materials, Journal Year: 2024, Volume and Issue: 41(3), P. 248 - 264
Published: Dec. 13, 2024
Language: Английский
ACS Applied Bio Materials, Journal Year: 2025, Volume and Issue: unknown
Published: Feb. 28, 2025
Patients and healthcare systems face significant social financial challenges due to the increasing number of individuals with chronic external internal wounds that fail heal. The complexity healing process remains a serious health concern, despite effectiveness conventional wound dressings in promoting healing. Recent advancements materials science fabrication techniques have led development innovative enhance To further expedite process, novel approaches such as nanoparticles, 3D-printed dressings, biomolecule-infused emerged, along cell-based methods. Additionally, gene therapy technologies are being harnessed generate stem cell derivatives more functional, selective, responsive than their natural counterparts. This review highlights potential biomaterials, 3D bioprinting, gene- therapies However, it also underscores necessity for research address existing integrate these strategies into standard clinical practice.
Language: Английский
Citations
2Journal of Oral Biosciences, Journal Year: 2025, Volume and Issue: unknown, P. 100622 - 100622
Published: Jan. 1, 2025
Language: Английский
Citations
0International Endodontic Journal, Journal Year: 2025, Volume and Issue: unknown
Published: March 28, 2025
Abstract Aim Dental pulp stem cells (DPSCs) are essential for regeneration but face low survival rates after transplantation. Genetic modification before transplantation is a promising solution to this issue. We aim elucidate the biological function and regulatory mechanism of hypoxic lncRNA HRL‐SC in DPSCs. Methodology The functions hypoxia inducible factor‐1α (HIF‐1α) DPSCs were evaluated vitro by cell proliferation, migration tube formation assays. Subcutaneous nude mice was used evaluate effect on DPSC viability vivo. RNA sequencing bioinformatics analysis, immunoprecipitation, dual luciferase reporter gene assay, co‐immunoprecipitation, fluorescence situ hybridization, immunofluorescence protein stability assays explore potential Data analysed one‐way analysis variance ( anova ) or Student's t ‐test, with p <.05 indicating statistical significance. Results HRL‐SC, hypoxia‐responsive lncRNA, enhanced abilities dental blocks revealed that HRL‐SC‐mediated exhibited improved elevated expression Ki‐67 CD31, along capacity form vascular‐like structures. HIF‐1α observed induce transcription HRL‐SC. Reciprocally, bound VHL, thereby inhibiting VHL‐mediated ubiquitination, which resulted positive feed‐forward loop HRL‐SC/HIF‐1α. RNA‐sequencing functional analyses closely associated hypoxia, angiogenesis, regeneration, integrin PI3K/AKT signalling pathways. Furthermore, shown stabilize ITGAV ITGB3 through PTBP1. Finally, it confirmed activated pathway via αvβ3/FAK HIF‐1α/PDK1 axes. Conclusions modified demonstrated signaling characteristics endothelial cells, may provide novel strategy application regeneration.
Language: Английский
Citations
0Dental Materials, Journal Year: 2024, Volume and Issue: 41(3), P. 248 - 264
Published: Dec. 13, 2024
Language: Английский
Citations
1