Enhanced Bioprinting of 3D Corneal Stroma Patches with Reliability, Assessing Product Consistency and Quality through Optimized Electron Beam Sterilization

Advanced Healthcare Materials, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 10, 2025

This study focuses on the optimization of sterilization methods for bioprinted three-dimensional (3D) corneal stroma patches prepared using cornea-derived decellularized extracellular matrix (Co-dECM) hydrogels and human keratocytes, with aim enhancing clinical applications in tissue engineering. An essential aspect this is to refine processes, particularly focusing electron beam (EB) sterilization, maintain structural functional integrity Co-dECM while ensuring sterility. The reveals that EB outperformed traditional like ethylene oxide (EtO) gas autoclaving, which tend degrade biochemical properties hydrogels. By optimizing EB-sterilization process, mechanical characteristics needed successful 3D bioprinting are retained, reducing batch variability patches. Consistency production vital meeting regulatory standards patient safety. Moreover, investigates immunomodulatory sterilized hydrogels, emphasizing their potential minimize inflammatory responses, crucial maintaining keratocyte phenotype. These findings significantly advance biomedical engineering by providing a method preserves material integrity, minimizes immunogenicity, supports translation patches, offering promising alternative donor transplants synthetic substitutes.

Language: Английский

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Dual Crosslinkable Gelatin Glycidyl Methacrylate-based Hydrogel: Key Insights to Biophysical Properties and Applicability Towards 3D Printing

Regenerative Engineering and Translational Medicine, Journal Year: 2025, Volume and Issue: unknown

Published: April 22, 2025

Language: Английский

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The Future of Automated Tissue Engineering: Robotic‐Assisted Strategies for Complex 3D Tissue Bottom‐Up Assembly

Advanced Materials Technologies, Journal Year: 2025, Volume and Issue: unknown

Published: May 4, 2025

Abstract Over the years, need for novel solutions to replace damaged tissues has led development of new tissue‐engineering strategies. Bottom‐up approaches have gained interest mimicking hierarchical cellular organization and intricate nature tissues. Among these approaches, automated‐assisted techniques, such as robotic handling, potential precisely control spatial building blocks, allowing creation highly specific functional Recognizing handling in tissue engineering, this review provides an overview robot‐assisted bottom‐up engineering complex tissues, highlighting advantages limitations various systems currently being explored. To address growing field, also discusses key considerations assembly living while providing insights into future directions challenges rapidly evolving field.

Language: Английский

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Advances in intestinal epithelium and gut microbiota interaction

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: March 4, 2025

The intestinal epithelium represents a critical interface between the host and external environment, serving as second largest surface area in human body after lungs. This dynamic barrier is sustained by specialized epithelial cell types their complex interactions with gut microbiota. review comprehensively examines recent advances understanding bidirectional communication cells microbiome. We briefly highlight role of various types, such Paneth cells, goblet enteroendocrine maintaining homeostasis function. Gut microbiota-derived metabolites, particularly short-chain fatty acids bile acids, influence function integrity. Additionally, we emerging evidence sophisticated cooperation different special emphasis on interaction tuft microbial balance. Understanding these has important implications for developing targeted therapeutic strategies gastrointestinal disorders, including inflammatory bowel disease, metabolic colorectal cancer.

Language: Английский

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The rise of 3D bioprinting: from organs to personalized medicine

Patient-Oriented Medicine and Pharmacy, Journal Year: 2025, Volume and Issue: unknown

Published: March 7, 2025

Bioprinting has emerged as a groundbreaking technology in the realms of organ transplantation and regenerative medicine, addressing critical challenges such shortages tissue repair. This review explores technological advancements innovations bioprinting, highlighting state-of-the-art techniques, bioinks, applications engineering. Key milestones printing functional tissues, including vascularized transplantable prototypes, are discussed alongside role bioprinting personalized where patient-oriented models revolutionizing drug testing therapeutic strategies. Furthermore, this article examines ethical considerations associated with offering insights into its future potential to transform global healthcare.

Language: Английский

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Hydrogel-based 3D printing technology: From interfacial engineering to precision medicine

Advances in Colloid and Interface Science, Journal Year: 2025, Volume and Issue: 341, P. 103481 - 103481

Published: March 19, 2025

Language: Английский

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3D bioprinting for bile duct tissue engineering: current status and prospects

Frontiers in Bioengineering and Biotechnology, Journal Year: 2025, Volume and Issue: 13

Published: April 14, 2025

Bile duct disorders, including cholangiocarcinoma, primary sclerosing cholangitis, and iatrogenic injuries, pose significant clinical challenges due to limited regenerative capacity the complexity of biliary tree. In recent years, 3D bioprinting has emerged as a promising approach for bile tissue engineering by providing patient-specific geometries facilitating spatial organization cells, scaffolding materials, bioactive factors. This review presents comprehensive overview techniques engineering, focusing on fundamental principles, biomaterial selection, current achievements, key challenges, future perspectives. We systematically discuss latest technological breakthroughs, highlight emerging innovations such organoid-based strategies microfluidic-assisted printing, evaluate prospects translation. Finally, we outline main challenges—such biocompatibility vascularization, immunological barriers, standardization protocols, regulatory hurdles—and propose directions research, emphasizing multidisciplinary collaboration translational studies.

Language: Английский

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Bioprinting in Organ Transplantation: From Experimental Models to Clinical Prospects

Brazilian Journal of Transplantation, Journal Year: 2025, Volume and Issue: 28(1)

Published: April 25, 2025

Background: Bioprinting has emerged as an innovative technology in organ transplantation and regenerative medicine, aiming to address pressing challenges such the shortage of donor organs need for effective tissue repair. By leveraging advanced 3D printing techniques, bioprinting enables fabrication functional tissues with precise architectural biological properties. Methods: This review provides in-depth analysis latest advancements bioprinting, focusing on cutting-edge development bioinks, their applications engineering. It examines significant breakthroughs creation vascularized transplantable prototypes explores role personalized medicine. Results: The findings highlight transformative impact biomedical field, particularly drug testing, therapeutic modeling, patient-specific treatment strategies. Additionally, key challenges—including technological limitations, ethical concerns, regulatory considerations—are discussed provide a comprehensive understanding field’s progress potential obstacles. Conclusion: holds immense promise revolutionizing global healthcare by offering solutions shortages advancing However, continued research innovation are necessary overcome existing facilitate its clinical translation into mainstream medical practice.

Language: Английский

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Bioimpressão no Transplante de Órgãos: Dos Modelos Experimentais às Perspectivas Clínicas

Brazilian Journal of Transplantation, Journal Year: 2025, Volume and Issue: 28(1)

Published: April 25, 2025

Introdução: A bioimpressão despontou como uma tecnologia inovadora no transplante de órgãos e na medicina regenerativa, visando solucionar desafios urgentes, a escassez doadores necessidade reparo eficaz tecidos. Ao aproveitar as técnicas avançadas impressão 3D, permite fabricação tecidos funcionais com propriedades arquitetônicas biológicas precisas. Métodos: Esta revisão fornece análise detalhada dos últimos avanços em bioimpressão, concentrando-se ponta, desenvolvimento biotintas suas aplicações engenharia Ela examina os significativos criação protótipos vascularizados transplantáveis explora função da personalizada. Resultados: As descobertas destacam o impacto transformador campo biomédico, particularmente testes medicamentos, modelagem terapêutica estratégias tratamento específicas para paciente. Além disso, principais — incluindo limitações tecnológicas, preocupações éticas considerações regulatórias são discutidos fornecer compreensão abrangente do progresso possíveis obstáculos. Conclusão: é imensamente promissora revolucionar saúde global, oferecendo soluções avanço regenerativa. No entanto pesquisa inovação contínuas necessárias superar existentes facilitar sua tradução clínica prática médica convencional.

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