Three-Dimensional Bioprinted Gelatin—Genipin Hydrogels Enriched with hUCMSC-Derived Small Extracellular Vesicles for Regenerative Wound Dressings

Polymers, Journal Year: 2025, Volume and Issue: 17(9), P. 1163 - 1163

Published: April 24, 2025

Mesenchymal stromal cell-derived small extracellular vesicles (MSC-sEVs) have shown great promise in promoting tissue repair, including skin wound healing, but challenges like rapid degradation and short retention limited their clinical application. Hydrogels emerged as effective carriers for sustained EV release. Three-dimensional printing enables the development of personalized substitutes tailored to size shape. This study aimed develop 3D bioprinted gelatin–genipin hydrogels incorporating human umbilical cord MSC-sEVs (hUCMSC-sEVs) future healing applications. Gelatin (8% 10% w/v) were crosslinked with 0.3% genipin (GECL) improve stability. The evaluated suitability extrusion-based bioprinting physicochemical properties, such swelling ratio, hydrophilicity, enzymatic degradation, water vapor transmission rate (WVTR). Chemical characterization was performed using EDX, XRD, FTIR. hUCMSC-sEVs isolated via centrifugation tangential flow filtration (TFF) characterized. successfully demonstrated superior high a ratio ~500%, slower optimal WVTR. hUCMSC-sEVs, ranging from 50 200 nm, positive surface cytosolic markers. Adding 75 μg/mL hUCMSC-EVs into GECL significantly improved biocompatibility. These offer ideal properties demonstrating potential biomaterial scaffolds regeneration

Language: Английский

Mechanisms and potential of mesenchymal stem cell-derived exosomes for treating radiation-induced intestinal injury

Extracellular Vesicle, Journal Year: 2025, Volume and Issue: 5, P. 100078 - 100078

Published: April 8, 2025

Language: Английский

CBD-Conjugated BMP-Inhibiting Exosomes on Collagen Scaffold Dual-Target Achilles Tendon Repair: Synergistic Regeneration and Heterotopic Ossification Prevention

Materials Today Bio, Journal Year: 2025, Volume and Issue: 32, P. 101790 - 101790

Published: April 22, 2025

Language: Английский