Guiding Oligodendrocyte Progenitor Cell Maturation Using Electrospun Fiber Cues in a 3D Hyaluronic Acid Hydrogel Culture System DOI Creative Commons
Rachel A. Mazur, Kyle J. Lampe

ACS Biomaterials Science & Engineering, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 20, 2024

The current lack of therapeutic approaches to demyelinating disorders and injuries stems from a knowledge surrounding the underlying mechanisms myelination. This gap motivates development effective models study role environmental cues in oligodendrocyte progenitor cell (OPC) maturation. Such should focus on determining, which factors influence OPCs proliferate differentiate into mature myelinating oligodendrocytes (OLs). Here, we introduce hyaluronic acid (HA) hydrogel system composed cross-linked HA containing encapsulated fibers with swollen diameters similar axons (2.7 ± 0.2 μm). We tuned storage moduli simulate native brain tissue (200–2000 Pa) studied effects fiber presence OPC proliferation, metabolic activity, protein deposition, morphological changes gels intermediate modulus (800 0.3 Pa). fiber-containing at culture days 4 7 exhibited significantly greater number process extensions, change associated differentiation. By contrast, fiber-free control maintained more proliferative phenotypes 2.2-fold higher proliferation day 1.8-fold activity 7. Fibers were also found extracellular matrix (ECM) deposition distribution, more, distributed, nascent ECM occurring gels. Overall, these data indicate that inclusion appropriately sized provides topographical cues, guide toward hydrogel/fiber is promising vitro scheme, providing valuable insight differentiation

Language: Английский

Oil‐Infused Polymer Fiber Membranes as Porous Patches for Long‐Term Skin Hydration and Moisturization DOI Creative Commons
Piotr K. Szewczyk, Michał Kopacz, Zuzanna J. Krysiak

et al.

Macromolecular Materials and Engineering, Journal Year: 2023, Volume and Issue: 309(2)

Published: Nov. 23, 2023

Abstract Skin allergies and diseases, including atopic dermatitis (AD), affect millions worldwide. Current treatments for AD are often expensive, leading to a need cost‐effective solutions. Here, using fiber‐based patches maintain increase skin hydration is explored, which helps treat eczema AD. Nanofiber membranes manufactured via electrospinning of eight different polymers: nylon 6 (PA6), polyimide (PI), poly(3‐hydroxybuty‐rate‐ co ‐3‐hydroxyvalerate (PHBV), poly( l ‐lactide) (PLLA), polycaprolactone (PCL), polystyrene (PS), two molecular weights poly(vinyl butyral‐ ‐vinyl alcohol‐ acetate) (PVB). Further, their morphology examined through scanning electron microscopy (SEM), fibers, pores diameter, wettability, membrane thickness. Additionally, water vapor transmission rates (WVTR) measured, notably, tests conducted before after evening primrose oil‐infused patches. The comparison findings highlight the flexibility electrospun patches, demonstrating potential in maintaining h enhancing moisture, necessary treatment. These insights, focus on selecting most effective performance help improve tailored AD, can significantly impact efforts reduce healthcare costs simplify skincare steps.

Language: Английский

Citations

4
Guiding Oligodendrocyte Progenitor Cell Maturation Using Electrospun Fiber Cues in a 3D Hyaluronic Acid Hydrogel Culture System DOI Creative Commons
Rachel A. Mazur, Kyle J. Lampe

ACS Biomaterials Science & Engineering, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 20, 2024

The current lack of therapeutic approaches to demyelinating disorders and injuries stems from a knowledge surrounding the underlying mechanisms myelination. This gap motivates development effective models study role environmental cues in oligodendrocyte progenitor cell (OPC) maturation. Such should focus on determining, which factors influence OPCs proliferate differentiate into mature myelinating oligodendrocytes (OLs). Here, we introduce hyaluronic acid (HA) hydrogel system composed cross-linked HA containing encapsulated fibers with swollen diameters similar axons (2.7 ± 0.2 μm). We tuned storage moduli simulate native brain tissue (200–2000 Pa) studied effects fiber presence OPC proliferation, metabolic activity, protein deposition, morphological changes gels intermediate modulus (800 0.3 Pa). fiber-containing at culture days 4 7 exhibited significantly greater number process extensions, change associated differentiation. By contrast, fiber-free control maintained more proliferative phenotypes 2.2-fold higher proliferation day 1.8-fold activity 7. Fibers were also found extracellular matrix (ECM) deposition distribution, more, distributed, nascent ECM occurring gels. Overall, these data indicate that inclusion appropriately sized provides topographical cues, guide toward hydrogel/fiber is promising vitro scheme, providing valuable insight differentiation

Language: Английский

Citations

1