Optics Letters,
Journal Year:
2024,
Volume and Issue:
49(24), P. 7230 - 7230
Published: Dec. 2, 2024
Penicillin
G
detection
is
of
great
significance
in
medical
research
and
disease
diagnosis.
Liquid
crystal
(LC),
as
a
branch
sensitive
materials,
has
broad
application
prospect
the
field
biosensing.
Herein,
liquid
crystal-coated
silica
microbubble
resonator
(LC-MBR),
with
high
sensitivity
for
penicillin
detection,
been
proposed
demonstrated.
Whispering
gallery
mode
(WGM)
spectra
Q
factor
exceeding
10
6
were
excited
through
coupling
between
tapered
fiber
LC-MBR.
The
orientation
transition
LC
molecules,
combined
WGM
resonance,
amplified
target
information
triggered
wavelength
blueshifts.
LC-MBR
was
then
applied
pH
achieving
2.51
nm/pH
within
range
3.8–8.5.
This
also
successfully
employed
to
detect
enzymatic
reactions
penicillinase,
limit
×
3
U/mL.
fabricated
sensor
offers
significant
advantages,
including
simple
structure,
improved
stability,
higher
across
wide
range,
making
it
promising
candidate
future
biomedical
applications.
Journal of Basic Microbiology,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Feb. 2, 2025
ABSTRACT
Soil
ecosystems
consist
of
diverse
microbial
communities
with
great
potential
for
ecological
and
biotechnological
applications.
These
encounter
various
abiotic
stresses,
which
expedite
the
activation
transient
overexpression
heat
shock
proteins
(HSPs).
In
present
study,
a
soil
bacterium
was
isolated
identified
as
Bacillus
licheniformis
strain
PSK.A1,
its
growth
parameters
were
optimized
before
exposing
it
to
heat,
salt,
pH,
antibiotic
stress
conditions.
Comparative
protein
expression
analyzed
using
SDS‐PAGE,
stabilization
via
aggregation
assays,
survival
through
single
spot
dilution
colony‐counting
methods
under
The
pre‐treatment
short
dosage
showed
endured
overall
tolerance
lethal
conditions,
evidenced
by
moderately
enhanced
total
soluble
intracellular
content,
better
stabilization,
comparatively
over‐expressed
HSPs,
relatively
cell
survival.
findings
highlighted
that
cells
grown
optimal
conditions
more
susceptible
environments
than
stressed
cells,
their
linked
20
distinct
HSPs
17–91
kD.
insights
offer
developing
strategies
enhance
resilience
applications
including
bacterial
bioprocessing,
bio‐remediation,
infectious
disease
management.
