Preclinical validation of electrospun fibers to achieve vaginal colonization by Lactobacillus crispatus DOI Creative Commons

Christy Armstrong,

Sudeshna Saha,

Marnie A. Aagard

et al.

Frontiers in Bacteriology, Journal Year: 2025, Volume and Issue: 4

Published: April 16, 2025

Introduction Communities of bacteria collectively known as the vaginal microbiota reside in human vagina. Bacterial vaginosis (BV) describes an imbalance this microbiota, affecting more than 25% women worldwide, and is linked to health problems such infertility, cervical cancer, preterm birth. Following antibiotic treatment, BV becomes recurrent many individuals. Lactobacillus crispatus widely believed contribute a healthy microbiome, its therapeutic application has shown promise early clinical trials investigating adjunct therapies for lasting treatment conditions BV. There pressing need platforms that apply biologically active agents probiotic bacteria, vagina with little user effort but effect. Methods Here, we use mouse model investigate functional utility potential harms soft, slow-dissolving fibers made by electrospinning polyethylene oxide (PEO) poly(lactic- co -glycolic acid) (PLGA). Blank electrospun passed quality control checkpoints were administered vaginally murine compared animals receiving mock procedures. Results Fiber administration had no significant effects on mucus glycan markers, epithelial exfoliation, keratinization, tissue edema or neutrophil infiltration. L. crispatus- loaded enabled colonization most one week. Mice -loaded significantly higher measured concentrations lactate washes at 48 hrs pre-colonization washes. Discussion These data provide pre-clinical proof concept can achieve viable delivery metabolically , without eliciting inflammation injury.

Language: Английский

Dual probiotic and antibiotic therapy targeting bacterial vaginosis: an integrated experimental/computational modeling perspective DOI Creative Commons
Bassam Fotouh, Anthony J. Kyser, Mohamed Y. Mahmoud

et al.

Biomedical Engineering Advances, Journal Year: 2025, Volume and Issue: unknown, P. 100163 - 100163

Published: April 1, 2025

Language: Английский

Citations

0
Preclinical validation of electrospun fibers to achieve vaginal colonization by Lactobacillus crispatus DOI Creative Commons

Christy Armstrong,

Sudeshna Saha,

Marnie A. Aagard

et al.

Frontiers in Bacteriology, Journal Year: 2025, Volume and Issue: 4

Published: April 16, 2025

Introduction Communities of bacteria collectively known as the vaginal microbiota reside in human vagina. Bacterial vaginosis (BV) describes an imbalance this microbiota, affecting more than 25% women worldwide, and is linked to health problems such infertility, cervical cancer, preterm birth. Following antibiotic treatment, BV becomes recurrent many individuals. Lactobacillus crispatus widely believed contribute a healthy microbiome, its therapeutic application has shown promise early clinical trials investigating adjunct therapies for lasting treatment conditions BV. There pressing need platforms that apply biologically active agents probiotic bacteria, vagina with little user effort but effect. Methods Here, we use mouse model investigate functional utility potential harms soft, slow-dissolving fibers made by electrospinning polyethylene oxide (PEO) poly(lactic- co -glycolic acid) (PLGA). Blank electrospun passed quality control checkpoints were administered vaginally murine compared animals receiving mock procedures. Results Fiber administration had no significant effects on mucus glycan markers, epithelial exfoliation, keratinization, tissue edema or neutrophil infiltration. L. crispatus- loaded enabled colonization most one week. Mice -loaded significantly higher measured concentrations lactate washes at 48 hrs pre-colonization washes. Discussion These data provide pre-clinical proof concept can achieve viable delivery metabolically , without eliciting inflammation injury.

Language: Английский

Citations

0