Design and Characterization of Peptide-Conjugated Solid Lipid Nanoparticles for Targeted MRI and SPECT Imaging of Breast Tumors DOI Creative Commons

Tahereh Rahdari,

Hossein Ghafouri, Sorour Ramezanpour

et al.

ACS Omega, Journal Year: 2025, Volume and Issue: unknown

Published: April 22, 2025

Triple-negative breast cancer (TNBC) presents significant challenges due to its aggressive behavior and lack of targeted treatments. High-resolution imaging techniques nanoparticles offer potential solutions for early detection monitoring TNBC. In this study, we developed characterized solid lipid (SLNs) conjugated with a C-peptide derived from endostatin target integrin αvβ3, overexpressed in These SLNs were loaded superparamagnetic iron oxide (SPIONs) enhanced magnetic resonance (MRI) radiolabeled technetium-99m (99mTc) single-photon emission computed tomography (SPECT), enabling dual-modality imaging. Extensive characterization the was performed utilizing variety advanced techniques, including dynamic light scattering (DLS), differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), X-ray diffraction (XRD), vibrating sample magnetometry (VSM), field-emission electron microscopy (FE-SEM), atomic force (AFM). This comprehensive validated successful synthesis functionalization nanoparticles, along their remarkable properties, while also revealing distinct spherical morphology, optimal size, uniform distribution, colloidal stability. The conjugation significantly targeting efficiency vitro, as evidenced by MTT receptor-binding assays 4T1 cells using flow cytometry MRI. vivo studies murine model demonstrated that peptide-conjugated accumulated tumor tissues, providing superior contrast MRI tumor-specific localization SPECT Biodistribution confirmed reduced off-target accumulation, particularly liver, compared nontargeted formulations. Collectively, C-peptide-conjugated provide promising platform TNBC, offering improved diagnostic accuracy targeting.

Language: Английский

Design and Characterization of Peptide-Conjugated Solid Lipid Nanoparticles for Targeted MRI and SPECT Imaging of Breast Tumors DOI Creative Commons

Tahereh Rahdari,

Hossein Ghafouri, Sorour Ramezanpour

et al.

ACS Omega, Journal Year: 2025, Volume and Issue: unknown

Published: April 22, 2025

Triple-negative breast cancer (TNBC) presents significant challenges due to its aggressive behavior and lack of targeted treatments. High-resolution imaging techniques nanoparticles offer potential solutions for early detection monitoring TNBC. In this study, we developed characterized solid lipid (SLNs) conjugated with a C-peptide derived from endostatin target integrin αvβ3, overexpressed in These SLNs were loaded superparamagnetic iron oxide (SPIONs) enhanced magnetic resonance (MRI) radiolabeled technetium-99m (99mTc) single-photon emission computed tomography (SPECT), enabling dual-modality imaging. Extensive characterization the was performed utilizing variety advanced techniques, including dynamic light scattering (DLS), differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), X-ray diffraction (XRD), vibrating sample magnetometry (VSM), field-emission electron microscopy (FE-SEM), atomic force (AFM). This comprehensive validated successful synthesis functionalization nanoparticles, along their remarkable properties, while also revealing distinct spherical morphology, optimal size, uniform distribution, colloidal stability. The conjugation significantly targeting efficiency vitro, as evidenced by MTT receptor-binding assays 4T1 cells using flow cytometry MRI. vivo studies murine model demonstrated that peptide-conjugated accumulated tumor tissues, providing superior contrast MRI tumor-specific localization SPECT Biodistribution confirmed reduced off-target accumulation, particularly liver, compared nontargeted formulations. Collectively, C-peptide-conjugated provide promising platform TNBC, offering improved diagnostic accuracy targeting.

Language: Английский

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