Glycan-Matchmade Multivalent Decoration of Enzyme Labels for Amplified Electrochemical Detection of Glycoproteins

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 28, 2025

Glycoproteins are of significant value to liquid biopsy human diseases. Herein, we present a universal electrochemical platform for the amplified detection glycoproteins, taking advantage glycan-matchmade multivalent decoration enzyme labels enzymatic signal amplification. Briefly, involves two steps, i.e., site-directed phenylboronic acid-coated gold nanoparticles (PBA-AuNPs) cis-diol-containing glycans glycoproteins and subsequent via affinity cross-linking between glycan moieties remaining PBA groups on PBA-AuNP cross-linkers. As matchmaking-based strategy enables each glycoprotein with multiple labels, this exhibits high sensitivity toward detection. Using alkaline phosphatase (ALP) as proof-of-concept label in combination solid-state voltammetric stripping assay enzymatically deposited metallic silver, limits at pg mL-1 level have been obtained aptamer-based thrombin prostate-specific antigen. Overall, work illustrates an efficient versatile ultralow concentration levels, holding desirable advantages simplicity cost-effectiveness over sandwich enzyme-linked immunosorbent assays.

Language: Английский

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Phenylboronic acid-functionalized biomaterials for improved cancer immunotherapy via sialic acid targeting

Advances in Colloid and Interface Science, Journal Year: 2024, Volume and Issue: 333, P. 103301 - 103301

Published: Sept. 6, 2024

Language: Английский

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Lectin-Mediated Labeling of Alkaline Phosphatase for Enzymatic Silver Deposition-Based Electrochemical Detection of Glycoprotein Tumor Markers

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 23, 2025

The screening of glycoprotein markers has become an integral part the in vitro diagnosis malignant tumors. Herein, electrochemical method based on alkaline phosphatase (ALP)-mediated enzymatic silver deposition is reported for highly sensitive detection tumor markers, which ALP enzymes are decorated to glycan moieties targets via lectin-carbohydrate interactions. As glycoproteins conjugated with multiple chains, lectin-mediated labeling can result decoration each target enzymes. Moreover, hydrolysis ascorbic acid 2-phosphate into a high density particles, then be sensitively assayed robust Ag/AgCl solid-state voltammetric process. result, deposition-based exhibits sensitivity. Using aptamer-based breast cancer-associated CA15-3 as proof concept, limit 0.32 mU/mL been demonstrated. Results show that synergism capture and glycoform-specific discrimination capability interactions endow this selectivity, its practical use assay levels serum samples illustrated. With benefits from efficiency, mild reaction conditions, user-friendly operation, applicable low-abundance markers.

Language: Английский

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Boronate crosslinking-based ratiometric electrochemical assay of glycated albumin

Talanta, Journal Year: 2024, Volume and Issue: 274, P. 125990 - 125990

Published: March 25, 2024

Language: Английский

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Aptasensor based on gold nanostructure-decorated 2D Cu metal–organic framework nanosheets for highly sensitive and specific electrochemical lipopolysaccharide detection

Microchimica Acta, Journal Year: 2024, Volume and Issue: 191(8)

Published: Aug. 1, 2024

Language: Английский

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Glycan-Evocated Metallization for Amplification-Free Electrochemical Detection of Glycoproteins at Low Concentration Levels

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(44), P. 17739 - 17745

Published: Oct. 21, 2024

Glycoproteins have become the most often screened tumor markers in vitro diagnostics. Although a large number of electrochemical methods been proposed to sensitively detect glycoproteins, them involve aid laborious signal amplification. Herein, we report use glycan-evocated metallization (GlyMetal) for amplification-free detection glycoproteins at low concentration levels. Briefly, interest are captured by an aptamer recognition layer, and then glycans targets oxidized NaIO

Language: Английский

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Site-Directed Electrochemical Grafting for Amplified Detection of Antibody Pharmaceuticals

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(22), P. 9278 - 9284

Published: May 20, 2024

Antibody pharmaceuticals have become the most popular immunotherapeutic drugs and are often administered with low serum drug dosages. Hence, development of a highly sensitive method for quantitative assay antibody levels is great importance to individualized therapy. On basis dual signal amplification by glycan-initiated site-directed electrochemical grafting polymer chains (glyGPC), we report herein novel strategy amplified detection pharmaceuticals. The target interest was affinity captured DNA aptamer ligand, then glycans were decorated alkyl halide initiators (AHIs) via boronate cross-linking, followed ferrocenyl from through electrochemically controlled atom transfer radical polymerization (eATRP). As can be multiple AHIs grafted composed tens hundreds electroactive tags, glyGPC-based permits dually In presence trastuzumab (Herceptin) as target, achieved limit 71.5 pg/mL. Moreover, developed selective, results in human satisfactory. Owing its uncomplicated operation cost-effectiveness, shows promise

Language: Английский

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Review of advances in Glycan analysis on exosomes, cancer cells, and circulating cancer-derived glycoproteins with an emphasis on electrochemistry

Analytica Chimica Acta, Journal Year: 2024, Volume and Issue: 1336, P. 343277 - 343277

Published: Sept. 26, 2024

Language: Английский

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Biologically-driven RAFT polymerization-amplified platform for electrochemical detection of antibody drugs

Talanta, Journal Year: 2024, Volume and Issue: 285, P. 127431 - 127431

Published: Dec. 19, 2024

Language: Английский

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