Combined Effects of Ketogenic Diet and Aerobic Exercise on Skeletal Muscle Fiber Remodeling and Metabolic Adaptation in Simulated Microgravity Mice DOI Creative Commons
Jun Chen,

Wenjiong Li,

Yu Liang

et al.

Metabolites, Journal Year: 2025, Volume and Issue: 15(4), P. 270 - 270

Published: April 13, 2025

Objective: Prolonged microgravity environments impair skeletal muscle homeostasis by triggering fiber-type transitions and metabolic dysregulation. Although exercise nutritional interventions may alleviate disuse atrophy, their synergistic effects under conditions remain poorly characterized. This study investigated the of an 8-week ketogenic diet combined with aerobic in hindlimb-unloaded mice on fiber remodeling adaptation. Methods: Seven-week-old male C57BL/6J were randomly divided into six groups: normal control (NC), hindlimb unloading (NH), (NHE), (KC), (KH), (KHE). During last two weeks intervention, was applied to simulate microgravity. Aerobic groups performed moderate-intensity treadmill running (12 m/min, 60 min/day, 6 days/week) for 8 weeks. Body weight, blood ketone, glucose levels measured weekly. Post-intervention assessments included respiratory exchange ratio (RER), exhaustive performance tests, biochemical analyses parameters. The composition evaluated via immunofluorescence staining, lipid deposition assessed using Oil Red O glycogen content analyzed Periodic Acid–Schiff (PAS) gene expression quantified quantitative real-time PCR (RT-qPCR). Results: Hindlimb significantly decreased body induced reduced endurance mice. However, combination KD attenuated these adverse effects, as evidenced increased proportions oxidative fibers (MyHC-I) glycolytic (MyHC-IIb). Additionally, this intervention upregulated metabolism-associated genes, including CPT-1b, HADH, PGC-1α, FGF21, enhancing metabolism ketone utilization. These adaptations corresponded improved performance, demonstrated time exhaustion KHE group compared other groups. Conclusions: a effectively ameliorates simulated microgravity-induced atrophy impairment, primarily promoting transition from MyHC-IIb MyHC-I (CPT-1b, PGC-1α). findings underscore potential therapeutic value dietary mitigating conditions.

Language: Английский

Combined Effects of Ketogenic Diet and Aerobic Exercise on Skeletal Muscle Fiber Remodeling and Metabolic Adaptation in Simulated Microgravity Mice DOI Creative Commons
Jun Chen,

Wenjiong Li,

Yu Liang

et al.

Metabolites, Journal Year: 2025, Volume and Issue: 15(4), P. 270 - 270

Published: April 13, 2025

Objective: Prolonged microgravity environments impair skeletal muscle homeostasis by triggering fiber-type transitions and metabolic dysregulation. Although exercise nutritional interventions may alleviate disuse atrophy, their synergistic effects under conditions remain poorly characterized. This study investigated the of an 8-week ketogenic diet combined with aerobic in hindlimb-unloaded mice on fiber remodeling adaptation. Methods: Seven-week-old male C57BL/6J were randomly divided into six groups: normal control (NC), hindlimb unloading (NH), (NHE), (KC), (KH), (KHE). During last two weeks intervention, was applied to simulate microgravity. Aerobic groups performed moderate-intensity treadmill running (12 m/min, 60 min/day, 6 days/week) for 8 weeks. Body weight, blood ketone, glucose levels measured weekly. Post-intervention assessments included respiratory exchange ratio (RER), exhaustive performance tests, biochemical analyses parameters. The composition evaluated via immunofluorescence staining, lipid deposition assessed using Oil Red O glycogen content analyzed Periodic Acid–Schiff (PAS) gene expression quantified quantitative real-time PCR (RT-qPCR). Results: Hindlimb significantly decreased body induced reduced endurance mice. However, combination KD attenuated these adverse effects, as evidenced increased proportions oxidative fibers (MyHC-I) glycolytic (MyHC-IIb). Additionally, this intervention upregulated metabolism-associated genes, including CPT-1b, HADH, PGC-1α, FGF21, enhancing metabolism ketone utilization. These adaptations corresponded improved performance, demonstrated time exhaustion KHE group compared other groups. Conclusions: a effectively ameliorates simulated microgravity-induced atrophy impairment, primarily promoting transition from MyHC-IIb MyHC-I (CPT-1b, PGC-1α). findings underscore potential therapeutic value dietary mitigating conditions.

Language: Английский

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