From nanotechnology to AI: The next generation of CRISPR-based smart biosensors for infectious disease detection DOI

Irkham Irkham,

Abdullahi Umar Ibrahim, Chidi Wilson Nwekwo

et al.

Microchemical Journal, Journal Year: 2024, Volume and Issue: 208, P. 112577 - 112577

Published: Dec. 25, 2024

Language: Английский

Multidrug-Resistant tuberculosis DOI

Dika Apriliana Wulandari,

Yeni Wahyuni Hartati, Abdullahi Umar Ibrahim

et al.

Clinica Chimica Acta, Journal Year: 2024, Volume and Issue: 559, P. 119701 - 119701

Published: May 1, 2024

Language: Английский

Citations

15
Genomic synergy in food traceability: Enhancing global food safety and security DOI
Jacob Tizhe Liberty

Ecological Genetics and Genomics, Journal Year: 2025, Volume and Issue: 34, P. 100324 - 100324

Published: Jan. 5, 2025

Language: Английский

Citations

1
Electrochemical Sensor for Biological Samples Monitoring DOI Creative Commons
Duygu Zabitler,

Esra Ülker,

Kübra Turan

et al.

Topics in Catalysis, Journal Year: 2025, Volume and Issue: unknown

Published: March 13, 2025

Language: Английский

Citations

1
CRISPR-based electrochemical biosensors: an alternative for point-of-care diagnostics? DOI
Dagwin Wachholz, Lauro T. Kubota

Talanta, Journal Year: 2024, Volume and Issue: 278, P. 126467 - 126467

Published: June 28, 2024

Language: Английский

Citations

7
Low-cost inkjet-printed nanostructured biosensor based on CRISPR/Cas12a system for pathogen detection DOI Creative Commons
Angela Gilda Carota, Andrea Bonini, Massimo Urban

et al.

Biosensors and Bioelectronics, Journal Year: 2024, Volume and Issue: 258, P. 116340 - 116340

Published: April 25, 2024

The escalating global incidence of infectious diseases caused by pathogenic bacteria, especially in developing countries, emphasises the urgent need for rapid and portable pathogen detection devices. This study introduces a sensitive specific electrochemical biosensing platform utilising cost-effective electrodes fabricated inkjet-printing gold silver nanoparticles on plastic substrate. biosensor exploits CRISPR/Cas12a system detecting DNA sequence selected from genome target pathogen. Upon detection, trans-activity Cas12a/gRNA is triggered, leading to cleavage rationally designed single-strand reporters (linear hairpin) labelled with methylene blue (ssDNA-MB) bound electrode surface. In principle, this sensing mechanism can be adapted any bacterium choosing proper guide RNA its DNA. biosensor's performance was assessed two representative pathogens (a Gram-negative, Escherichia coli, Gram-positive, Staphylococcus aureus), results obtained inkjet-printed were compared those commercial screen-printed electrodes. Our show that use nanostructured electrodes, which provide large surface area, combination hairpin containing poly-T loop increase sensitivity assay corresponding signal variation 86%. targets amplified various clinically isolated have been tested demonstrate potential proposed point-of-need applications.

Language: Английский

Citations

6
Signal-amplification strategy and advances in electrochemistry-based CRISPR/Cas12 biosensing DOI
Likun Pan, Weiwei Jiang, Fei Deng

et al.

Chemical Engineering Journal, Journal Year: 2025, Volume and Issue: unknown, P. 161110 - 161110

Published: Feb. 1, 2025

Language: Английский

Citations

0
Advances in CRISPR/Cas13a-based biosensors for non-coding RNA detection DOI
Tao Zhu, Weiwei Jiang,

Yingyu Wu

et al.

Talanta, Journal Year: 2025, Volume and Issue: 294, P. 128223 - 128223

Published: April 25, 2025

Language: Английский

Citations

0
Advances in electrochemical biosensors employing carbon-based electrodes for detection of biomarkers in diabetes mellitus DOI Creative Commons

Serly Zuliska,

Iman Permana Maksum, Yasuaki Einaga

et al.

ADMET & DMPK, Journal Year: 2024, Volume and Issue: 12(3), P. 487 - 527

Published: July 24, 2024

The increase in diabetes cases has become a major concern the healthcare sector, necessitating development of efficient and minimal diagnostic methods. This study aims to provide comprehensive examination electrochemical biosensors for detecting mellitus biomarkers, with special focus on utilization carbon-based electrodes.

Language: Английский

Citations

3
CRISPR/Cas12a dual-mode biosensor for Staphylococcus aureus detection via enzyme-free isothermal amplification DOI

Hongmin Gao,

Hehua Zhang,

Xue Qi

et al.

Talanta, Journal Year: 2024, Volume and Issue: 282, P. 127013 - 127013

Published: Oct. 10, 2024

Language: Английский

Citations

3
Recent Advances in Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-Associated Proteins System-Based Biosensors DOI Creative Commons

Xiaonan Xin,

Jing Su, Haoran Cui

et al.

Biosensors, Journal Year: 2025, Volume and Issue: 15(3), P. 155 - 155

Published: March 2, 2025

High-sensitivity and high-specificity biodetection is critical for advancing applications in life sciences, biosafety, food safety, environmental monitoring. CRISPR/Cas systems have emerged as transformative tools biosensing due to their unparalleled specificity, programmability, unique enzymatic activities. They exhibit two key cleavage behaviors: precise ON-target guided by specific protospacers, which ensures accurate target recognition, bystander activity triggered upon binding, enables robust signal amplification. These properties make highly versatile designing biosensors ultra-sensitive detection. This review comprehensively explores recent advancements system-based biosensors, highlighting impact on improving performance. We discuss the integration of with diverse readout mechanisms, including electrochemical, fluorescent, colorimetric, surface-enhanced Raman scattering (SERS), so on. Additionally, we examine development integrated systems, such microfluidic devices portable leverage technology point-of-care testing (POCT) high-throughput analysis. Furthermore, identify unresolved challenges, aiming inspire innovative solutions accelerate translation these technologies into practical diagnostics, food, environment safety.

Language: Английский

Citations

0